LOCUS       sequence-426504-        1536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of mitoEcSTH 
            which is a soluble transhydrogenase from E. coli that can be used to
            increase NADH/NAD+ ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-mitoEcSTH-FLAG
FEATURES             Location/Qualifiers
     source          1..1536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1504..1527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaattcatgc tggccaccag ggtctttagc ctggtgggca aaagagccat ctccacatcc
       61 gtctgcgtga gagcccatcc ccacagctac gattacgacg ccatcgtgat cggcagcgga
      121 cccggtggag aaggcgctgc catgggttta gtcaagcaag gtgctagggt ggccgtcatt
      181 gagaggtacc agaatgtggg cggcggctgt acccactggg gaacaatccc tagcaaagct
      241 ttaaggcacg ccgtgtctcg tatcattgag ttcaaccaga accctttata ctccgaccac
      301 tctcgtttat taagaagcag cttcgccgac attttaaacc acgccgataa cgtcatcaac
      361 caacagacca gaatgaggca aggtttctac gagaggaacc actgcgagat tttacaaggt
      421 aacgctagat tcgtggacga acacacttta gccctcgact gccccgatgg ctccgtggag
      481 actttaacag ccgagaagtt cgtgattgct tgtggcagca gaccctacca ccccaccgac
      541 gtggacttca cccaccctcg tatctatgac agcgacagca ttttaagcat gcatcacgag
      601 cccagacacg tgctgatcta cggcgccggc gtgatcggct gcgagtacgc tagcatcttt
      661 cgtggaatgg acgtgaaggt ggatttaatc aacactcgtg ataggctgct cgcctttctg
      721 gatcaagaaa tgagcgattc tttaagctac cacttctgga actccggagt cgtgattcgt
      781 cacaacgagg agtacgagaa gattgagggc tgcgacgacg gcgtgatcat gcatttaaag
      841 tccggcaaga agctgaaggc cgactgttta ctctatgcca acggtcgtac cggcaacacc
      901 gactctttag ctctccagaa catcggactg gagaccgact ctcgtggcca gctgaaagtg
      961 aacagcatgt accagacagc ccagccccat gtgtacgccg tgggagatgt gatcggctac
     1021 ccttctttag cttccgccgc ttacgaccaa ggtcgtatcg ctgctcaagc tttagtcaaa
     1081 ggagaggcca ccgcccattt aatcgaggac attcccaccg gcatctacac catccccgag
     1141 atcagcagcg tgggcaagac agagcagcag ctgaccgcca tgaaagtccc ttacgaggtg
     1201 ggaagagccc agttcaaaca tttagctcgt gcccagatcg tgggcatgaa cgtgggcacc
     1261 ctcaagatcc tctttcatcg tgagaccaag gagattctgg gcatccactg tttcggagag
     1321 agagctgctg agatcatcca cattggccaa gctatcatgg aacagaaggg cggcggcaac
     1381 accatcgagt acttcgtgaa caccaccttc aactacccca ccatggccga agcctatcgt
     1441 gtggccgccc tcaatggttt aaacagactg ttcggtggat ctggtggatc tggtggatct
     1501 atggattaca aggatgacga tgacaagtaa accggt
//
