LOCUS       sequence-426509-        1488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLVX-TetOne-Puro-mitoLbNOX-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-mitoLbNOX-FLAG
FEATURES             Location/Qualifiers
     source          1..1488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1456..1479
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaattcatgc tcgctacaag ggtctttagc ctcgtcggaa agagagctat cagcacctcc
       61 gtctgcgtga gagctcataa ggtcaccgtg gtcggatgca cccatgccgg caccttcgcc
      121 atcaagcaaa tcctcgctga gcaccctgac gccgaggtca ccgtctacga gaggaacgat
      181 gtgatctcct tcctgtcctg tggcatcgcc ctctacctgg gcggaaaagt ggccgatccc
      241 caaggcctct tctacagctc ccctgaagaa ctgcagaagc tgggcgctaa tgtgcagatg
      301 aaccacaacg tgctggccat cgaccctgac caaaagaccg tcacagtcga ggacctcacc
      361 aatcacgccc agaccaccga gtcctacgac aaactggtga tgacctccgg aagctggcct
      421 atcgtgccca aaatccccgg catcgacagc gatagggtga agctctgcaa gaattgggcc
      481 cacgcccagg ctctgattga ggacgccaag gaggccaaga ggatcaccgt catcggcgcc
      541 ggatacatcg gagccgaact ggccgaggcc tactccacaa caggccacga cgtcaccctg
      601 attgacgcca tggctagggt catgcccaag tacttcgatg ccgacttcac cgacgtcatc
      661 gaacaggact acagggacca tggcgtgcaa ctcgctctgg gcgagacagt ggagagcttc
      721 accgacagcg ccaccggcct cacaatcaag acagacaaga actcctatga gaccgacctg
      781 gccatcctct gcattggctt taggcccaac acagacctgc tgaaaggcaa agtggacatg
      841 gcccctaacg gcgccatcat taccgacgac tacatgaggt ccagcaaccc tgatattttc
      901 gctgctggcg actccgccgc cgtccattac aaccccacac accaaaacgc ctacattccc
      961 ctcgctacca acgccgtcag gcagggaatc ctcgtcggaa agaacctcgt caagcccaca
     1021 gtgaagtaca tgggaaccca gtccagctcc ggactggccc tctatgacag gacaattgtc
     1081 tccacaggcc tcacactggc cgccgccaag caacaaggcc tcaatgccga gcaggtcatc
     1141 gtggaggaca actataggcc cgagttcatg ccttccaccg agcccgtcct catgagcctg
     1201 gtcttcgacc ccgatacaca cagaatcctg ggaggcgccc tgatgtccaa atacgacgtg
     1261 tcccagagcg ctaataccct gtccgtctgc atccagaacg agaacaccat cgatgacctg
     1321 gccatggtgg acatgctgtt ccagcccaat ttcgacaggc ccttcaacta cctgaacatt
     1381 ctcgcccagg ctgcccaagc taaagtggcc caatccgtca acgctggtgg atctggtgga
     1441 tctggtggat ctatggatta caaggatgac gatgacaagt aaaccggt
//
