LOCUS       sequence-426512-       12096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tn7 transposase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pTNS1
FEATURES             Location/Qualifiers
     source          1..12096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  8129..8353
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      8559..8947
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(9565..9936)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9969..10078
                     /note="incP origin of transfer"
     primer_bind     complement(10759..10778)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10917..10939)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11080..11099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(11156..11947,11948..12016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11779..11798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12017..12096,1..25))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagatctgc
       61 aggtcgacgg atcccaagct tcttctagag gtaccgcatg cgatatcgag ctctcccggg
      121 aattccagac tatgtcgaac tagataacgc ggaggagctg gccgacgcaa tcattcgaga
      181 ggttgagtta aaggaggcat ggctcacaag ggtcttgggg aactcagccg atcctgacgc
      241 ttctgtgggc aagtgggacc ggcgcctagc gcagtgcggt ggtattgatg agatgtcaag
      301 tggggtgcag gcaacatact tcagtccaaa cggtcagctg gtcggcttag tggcatacat
      361 tgatgccgaa tggatggatg aatagctctg acgttacgat tcaggtgaga caaccagatg
      421 ggcattgcgt acaagctcag ttgccaccga tgtggctacg agtctgatct tctttaccta
      481 ggccaaggta tggcgatgct tcctgagcaa atagtcggaa cgtgcacatg ctgcgacaat
      541 ctaacgacga tttctataaa tgaaagctac ggagtatgct cactttgtgg ggctaaggat
      601 cgtgtgatat ttacaagcta tcagcatgag gcaaggccaa gaaatccctg ctatctagag
      661 agaagtttca aatatcaatg cccaagatgc catgccttca gtatggagcc tccggatcta
      721 ccggagatgc tttgggacta atgctccgct ttgcctcggg tatccgttcg acctatgttt
      781 aagaggaaac gcacgtgtct gccgccaaag ctcttgccta tgcactttgt gacaagttgg
      841 aaagcaaaaa agttgatgaa gtcgtacgaa atcttgtttc tagcggctgg aatattaggg
      901 aagtggcctg ggaagcttcg tcctttgatc atgaaatgcc ctactttgac acggggcggg
      961 acttcgatat tggatttcct gacacgctgt catccaagat tgagtttttg accgcgaatg
     1021 gatcaatcgt ccaagcacgt gctcgcctgc acttcaagca ggctctaatt tttgccaagg
     1081 cgtccaaaca tgtcgccgcg ctcaagaact cattggatta ctactatgga tcgtctgtaa
     1141 tcccccctca aaaccacgga ggctatttgt agaaaattcc attaatatga actcaaggtg
     1201 ttccctacgg acgaatcagc gatgaaggtg gtgtacctgg ctatccaggc ggcgaccaag
     1261 aaatggacga tgccgatccg caactggaag ccggccatga accgttttat gatcgaattt
     1321 ggtgaccgtt taaatggcca cctttaactg accggcaaaa gcacttacac aaaagggttt
     1381 acaggctctt tcagtcattc ctttaatgcg taaattgctc tctaaccact ctctcagccc
     1441 acccttccat atcctctctc gatacttgca gctcccccaa cccctttgga tgattcaccc
     1501 ctctatgccc gtcttgtcca tataattgat caaacaaact atttggccaa ttcaatgagc
     1561 tcttcaccac tccacgccgt atcttttcaa aatcattccg ccatgtttcg ctatcaacgc
     1621 cactcaacac tttggtagaa agcattttca ccccatccga tgcatccacc tctagcaata
     1681 caaattcact gccattacgt tgcaccccaa cggctttaat cactcttggt gagccatctt
     1741 tacacagatg taatcggcta cgcccaactt ttggcaaaac caaggtttct tcaaaaagca
     1801 cccgacatgc aaatttagtt tttagaattg aaagtagctc atcaaaagca gcaaatcgat
     1861 tattttggct cgttgaagat ccgatgcttc aaactcaatc acacgatccg ccttatttat
     1921 ttgctttgtt gtactgttaa ccaccttagc tcgacttaat aaaggcaaat gagtaaatcg
     1981 agtccatttc cgttcttgca ttggattaaa ccaaacaaaa atacgccact ccttatgacc
     2041 cgagttacgg aataaatgac caatatgtac aacctgcaca ttgtcattaa atgtagctag
     2101 cctaaccact cactcttccc cataaaccat ttccaagaat ctttgtgctt cctcagttat
     2161 ccgctcttta gataacgttg cacttcttaa taatcgccag cgcctaagct caacatcttc
     2221 ctgtttaagc ttaataaaag cttggctaat ccgtctaatt tgataatctt ccacactctc
     2281 tgagtaacgc tttaagcaaa gcgctaccaa aggcagtttc tgtagatttt ttgcaagaga
     2341 ggttccgtta ggagtttgct ttaacagcca gctcgatgtc gctcttgggt gatcaaggct
     2401 actatctaga cgctttatga ttcttaatag ttgtcgtaca gcaattcgat ctctttggtt
     2461 ccaatcaact ctaggggcgg gtgccagacg ctcttgttga tgctgttgat tccagtgaac
     2521 tagccaatcc ctgtcatgtc ggtaaagcca agcgtatagc accccaccct ctaaagactg
     2581 tcttgccgcc ttaattcctt ggtatttatg cactagttgc tgccagtctt tatgcttaac
     2641 agataaatct tcagagttag gttgcacaga ctggctaaca ggtcttgtcg ttatagagtg
     2701 ctcagtaaga gcacttgcct gctgtagcgc ttctataacc gttagttttg gcaataaggc
     2761 ttgccacaca atactatgct gtaaataact aaaggctttt ctatgcttac ggaatatact
     2821 tttcagccaa cacgtgtcct tgttctctgc caactttaaa tccagtttct ctagtgcctc
     2881 atcactaaaa gtctgcctca ctctctccgc caccaagtca tgacgaatgt gcttgctttt
     2941 ggttagccct agatcctgcg ctaagcgctg ataaaacagc gtccactgct caaggcttgg
     3001 ggaaagctct tgcgctcgtg gagcatctaa cagaggggct atataagcag ctagtgctgt
     3061 taattgagat agggagtctt tggggtagtc tgaaagcagc tcagtatgac ccaaagccca
     3121 aaattgatgt cggtgatcat ctacagctct atcaaagaag actaaagcac cgtgttttgg
     3181 acaatatggc aaagcgggca aataccaatc tcgttgccaa aaggcttccc catacctatt
     3241 tagctgaaga gcaacgcaat cagggcagta tctaaagcgg ttatcgctct taactctaga
     3301 agcagcgact cctagcatta aatgcaccgc accttgcgct tggtactcca ttaaccgaat
     3361 agcttcgtct cggcgctcct tgcctacaaa cggagcatat aaagggaata aggtatgctc
     3421 ataaataagc tgctgaacag cgtaacgtcc tgtttgatgt agatgtcttg ctatcacacc
     3481 taaatgcgag ggcagaccta aggtagcgac caccttgcgg ttgccataca cctcatccaa
     3541 cagctgctta ggactaacaa tcccttgata aacgcctgcc cgtgcaatag tgctataaat
     3601 cagctcattc gagtacggaa caggaaagtt tctcatggct aacctgcttg tttaaataag
     3661 ctcgccatat ccactattac ccctttccct tttaaccgtt catgcatggt tttttcaggt
     3721 tggcgttgtg aatagatata acgtaaatcc gtatcaggca agctatccca gtcgctgggc
     3781 ttgactacct ttatagccga aaccttttta ctcttggagg gcttttctag ttctgacact
     3841 accgtttcgc cttccatcaa ccactgcaaa acaagaggca gtaacttttg tcttgtcatc
     3901 gttggattct ggctaaacgc ttttttaata gtgggaatta acaggcttga atcgtaatcc
     3961 tctttcagca tcagatataa atgacgctga tcttcggtat ctaactcttg aagggctttt
     4021 tcttctggtg tttgttcttg tatcgctgcg atatctagct gaagttggat taaccgttta
     4081 tcaatctcgg gaacgactag atcagaataa cgagcaatgc gttctgggat acccgagcgt
     4141 aatgcctcta gcatggggtg cacaggcttt aactcatctt gatacacttg ccgcaataaa
     4201 ccagcggtaa tacgctcatt gcctaaagct agcgcacgga gctgagcgag tacaaaaagt
     4261 tttactacaa tgtccatcac tccttggctt agctcatacc acacatcacg gacctcatcc
     4321 gataacagcg catctttgcg ttgtaaaagc tgtaattgcc aaagattatc cgtaaaagcg
     4381 atccactctt gattgggctt tccacgttgc gtttgttgta taggatccca gaatatagct
     4441 ccaaaccctg cccctctacg tgcagaccgc aaatcagcct caaaaatctc tcgtgcttta
     4501 ggggtaccaa tcaacatcac tggtacgcca ataatattca ccatcgtcac aaaaaagttc
     4561 agcatctctt gagatccacc cgaacgagag cggcttaaat gctgaatttc atcaataacc
     4621 aacaacccta aagcatgtgc attggctatt tgcgacatca aagccaacat ggtttctata
     4681 ccatgacgtt ttaagccata acgacgctca tagttcgagc ccaaggctcg atccaacgct
     4741 ctgaaaaaat tcaagcagat ttcttttagc gaaccattat gcgagcagtc tattttcaaa
     4801 tacaccacct gctctacatt gagttcacga tggtaaatca cctgaggata cgtggctaga
     4861 atacgatgaa gagaggtcgt cttcccacta ccagaacaac caattaacaa taagctttgt
     4921 gccgtagatc gtgcctcctc aaagcgaaat gtctccaact ctcccgtttg aacacgctca
     4981 taaccatttt gtaaatgctt ttgtaaatct cctgttttag gatttctgcc tacgtagcca
     5041 cctcgaatca tgaccgaaat acgctcactt agtagcaaat gcgtacctaa tggctgaaaa
     5101 tagtcatctg gaatacgaca aatggtatga gctctgataa cacgggactt ttgcaagtca
     5161 gaggaagtaa gctgtaaaga ggatttcagt gatgcagcac tattcactga ctcttgtaat
     5221 ggtggtaagg cctcgataaa aggattatca cgataagcct ctacccccgt atcacgataa
     5281 actgcttgaa tccgggtagc actcatgact catccttttc tggtggatcc tgaaataatt
     5341 caggcacgta tgtgggtagg ctgtaatctt cttgatcatc cgcttccact gcgttgaaag
     5401 gaataacttt agcctcatca cctgaagagc ttggcttcaa atgctccgca cgttttttac
     5461 gctccgaggt cacggcttct tttttattag ttttaatctg cttaatgcgt gttgatttgg
     5521 gctcagtagt actgggcgtt aacttattcg ctttctgaat ggtttgctga ataaacgcct
     5581 caagctccct gcgtttagtt aactcatcct gcttcgcatt ggctttattg tgtttttctt
     5641 gtgcttgtat atcccaaacc tcccaaaatg agagaccttt aaactgccga ctacgttccg
     5701 tcagattaca gcgccaaaat acacggctgc caacttgcgg aaacaaataa atcgtatcaa
     5761 ccagcactgg gtcataagcc gcttctaaat gttgaggtct agctatatca gtgctccgct
     5821 gcaaccaacc ctcacgcaga atctctgacc ccgagtaata caaaccccac aaattaacgc
     5881 caaatgaaga aatagagacc tttcggcgag gcagtaacgc tactcgcaac tgctcttgct
     5941 ccacagccct taaactacct gtacgatgct gcatacccca ttgccatagc tggacaggaa
     6001 tagacggtaa atctgtagga aaatcagcat ctcgatcgta tttatccatc accagatggt
     6061 tatttctgaa taagatcgta cgcaaaataa tttgtgtgaa ctcaaatacc gacagagatg
     6121 catctaacct atagtctgtt tcaccatggc ttttgatccg actgccctct acaatgccag
     6181 gtgcaaagga cttaaactcg gcttgtagtg ttctaaaagt gctttccact atgcctttag
     6241 catcgccacg tctaggtgga gcactttcca ctcgcacatt aaaactagaa actaaggctt
     6301 cgacctgatg actcattaat tcgccacggt ccgctagcaa cacatctggc aaacctacac
     6361 acggccagtc tgagctacta atctcaatat catgctgggc acaaatggcc gttttgtcag
     6421 agcaagcatt tacaaaagcc tgcatcgcca ccacataaga cggattttca aagccgatat
     6481 aaaagcccgt gatcatccga ctaaacacat caatcacaat gtaaagcgtt ggtcttccta
     6541 tgattttttg gcgatcatga tgatccacta aataaatatc agcaatcgtg gcatcaatct
     6601 cataacgact cccagggcct aacgcctgag aagtggctgt actacttaag ggtcgtacgt
     6661 cttttttata tacccctgct ttaactctag actttaagcg ctgagcttta gggtattctc
     6721 gatcataaaa ataacgaaac tgacgtagtg ttgggtaatc ctcttgggga atgcgaggaa
     6781 aatactgagc aaacaagtcc acaaatcgtc tataggcaac ggtggtcttt gtaccttttt
     6841 gatttaacag gtgcttttct atggtcaacc taaaaaggcg ttcaatctcg ggcgttacct
     6901 tggttccttc acccttacca tattctctgg ctcggccaat ctttgctgtt cctgtcgctg
     6961 aacgtctttc ccctggtgca ccgctgtttt tgtagtcagg aattaatgca ttaggcgttt
     7021 gaccacgctg ccagtaacgg cgtaacaact tataaactgt agccttagta accttatgtt
     7081 cttggaccac atgctcaacg agttcgcttc tgactttagg gtcgaaacga tccttactat
     7141 taataatagg aagaattttt cgataatcct cctcgcgctt ctggaagctg acagaatcta
     7201 cagacggctc ttctaaatca agatgtacat aagggtcatc agttctaact aagcggcctt
     7261 catctaaata ctgcattagc aacaactcag ccctagcttg tggaactcct ttatcagcgc
     7321 ttatttgcat ccagacaact tggccatcct ctatagccaa aatgcgatac ggatcattat
     7381 caaatagcac aacctcatta atttgccaca tagcgcaact cctccatatt cactacttgg
     7441 ctaatacaga gatctgcaca cttatttgcc ctgaaagact tataaatatt gaacttaata
     7501 aaaccatttg cggttaaggc tcgtatctca ctcaatgttt tgcctaactc caaatcataa
     7561 gcaatatcaa cctgcttaca gacattgata atgttttcat ctcctttttc ttgcaggata
     7621 tgggccaatg gggatagttg tgctaaaagc tccgcagaaa cttcttctgt tttcactgaa
     7681 taaagccatt caatattttc ttttactacg ggatttattt ctttatcagt aaaaatgaac
     7741 caaggaattt gcttttgctg ccaatagcga cgctctagtt ctagtttttc taaggtacgc
     7801 tcgtcttgta aggctgctgc aggtttgact tgaatagcaa actgctcaaa aggaccatct
     7861 ttgcagtcca ctaaaaaatc agtagacata acctgatcta caccacgaat aacaggatgc
     7921 ttaataccac tatctattgc aatctgcctg gtatcactag gtaataaggg gaactgctcg
     7981 cgtatatcta gcacgctgct ctcccactca agactgagaa aaacagcaag ctctaagtca
     8041 gatagcaaat gatggactcg tcccgtctta tgagaataaa tacggtggga acgacctgaa
     8101 gaaggaactt cttgtactgt tagccatgga atatagtctt taccatgccc ttggccacgc
     8161 ccctctttaa tacgacgggc aatttgcact tcagaaaatg aagagtttgc tttagccata
     8221 acaaaagtcc agtatgcttt ttcacagcat aactggactg atttcagttt acaactattc
     8281 tgtctagttt aagactttat tgtcatagtt tagatctatt ttgttcagtt taagacttta
     8341 ttgtccgccc acacccgctt acgcagggca tccatttatt actcaaccgt aaccgatttt
     8401 gccaggttac gcggctggtc aacgtcggtg cctttgatca gcgcgacatg gtaagccagc
     8461 agctgcagcg gaacggtgta gaagatcggt gcaatcacct cttccacatg cggcatctcg
     8521 actctagagg atccccgggt accgagctcg aattcccatg tcagccgtta agtgttcctg
     8581 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
     8641 tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct
     8701 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
     8761 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag
     8821 tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga
     8881 gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct
     8941 tcagatcctc tacgccggac gcatcgtggc cggatccagc cgaccaggct ttccacgccc
     9001 gcgtgccgct ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt ttcgtgattg
     9061 tcacgctcaa gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag ggcgcggtag
     9121 gcccgatacg gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat ggcgatatgg
     9181 atgtgcaggt tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc ggcgaagcca
     9241 agcccagcgc agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg cttctctccc
     9301 gcgcggaagc taaccagcag gtgataggtc ttgtcggcct cggaacgggt gttgccgtgc
     9361 tgggtcgcca tcacctcggc catgacagcg ggcagggtgt ttgcctcgca gttcgtgacg
     9421 cgcacgtgac ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
     9481 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
     9541 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
     9601 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
     9661 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
     9721 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
     9781 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
     9841 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
     9901 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     9961 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
    10021 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
    10081 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
    10141 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
    10201 tgcagcggaa aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg
    10261 caaatgcagg aaattactga actgagggga caggcgagag acgatgccaa agagctacac
    10321 cgacgagctg gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc
    10381 tgcggttgcg ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc
    10441 gctcgtcacc atttgggagc acatgcggga aacggggaag gtcaagttct cctacgagac
    10501 gttccgctcg cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc ccgcaccgca
    10561 ggccaaggct gcggaacccg cgccggcacc caagacgccg gagccacggc ggccgaagca
    10621 ggggggcaag gctgaaaagc cggcccccgc tgcggccccg accggcttca ccttcaaccc
    10681 aacaccggac aaaaaggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
    10741 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
    10801 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
    10861 gactgttggg cgccatctcc ttgctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10921 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    10981 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    11041 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    11101 tgagagtgca ccataaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11161 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11221 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11281 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11341 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11401 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11461 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11521 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11581 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11641 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11701 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11761 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11821 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11881 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11941 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12001 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12061 gtctcatgag cggatacata tttgaatgta tttaga
//
