LOCUS       sequence-426559-       10566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split hygromycinR landing pad insertion plasmid for ChrIII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Moerdyk-Schauwecker MJ, Jahahn EK, Munoz ZI, Robinson KJ, Phillips 
            PC
  TITLE     Expansion of the split hygromycin toolkit for transgene insertion in
            Caenorhabditis elegans.
  JOURNAL   MicroPubl Biol. 2024 Jan 29;2024:10.17912/micropub.biology.001091. 
            doi: 10.17912/micropub.biology.001091. eCollection 2024.
  PUBMED    38351905
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2024-01-29"; volume: 
            "2024:10.17912/micropub.biology.001091. doi"; pages: " 
            10.17912/micropub.biology.001091. eCollection 2024"
COMMENT     Sequence Label: pMS110
FEATURES             Location/Qualifiers
     source          1..10566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(99..118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(778..1569,1570..1638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1401..1420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1639..1743)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1811..1829
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1867..1889)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1989..2008
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    complement(4292..4325)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     terminator      complement(5764..6087)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    8005..8029
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8032..9057
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8032..9057
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(9058..9082)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9435..9468)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(10183..10199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10183..10199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10196..10218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10207..10223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10231..10237,10238..10255,10256..10261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10276..10297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10414..10431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
       61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1741 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1801 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     1861 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1921 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     1981 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     2041 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     2101 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     2161 atgtgctgca aggcgattaa gttgggtaac gcatgctgta aatggtcggt aaagatcaat
     2221 atcaaattgg gctctgaggg aatgactgaa aatccacttt ttctgcttga actcagcaga
     2281 tataattttt ttaaaaattt agtttttaaa ttctgaaaga acacaaaatt tgcaacaaaa
     2341 tggctacagg gatttttgaa aaagttagtt tgaagccaga tattaatttt taaaaattgg
     2401 aaaaactact aattttgcat ataaatggtc ggtttaatca atttcaaatt gtgcactgaa
     2461 atgtatgatt aaatatccac tttttctgct tgaacttagc ggatatgaaa cttttttaaa
     2521 tttagttttt aaattctgaa agaacacaaa atttgcaaca aaatagctac agggattttt
     2581 gaaaaagtta gtttgaagcc agatattcat ttttaaagtt tggaaaatct acatcgttaa
     2641 ctaatttctg aaaaacagga aaaagtcaaa taaatcgcaa aaatggaagt tcaaataaaa
     2701 aagttttgaa atttttccta atttttcaaa aacttatgca atgtgtatta aatgtataaa
     2761 tgtataaatt atgtattaat atgttgtgta ataaaaatgt attacctatc aattttgaag
     2821 caaagtagtt tgaaatttta atttcaggaa atttttgcac caacttactt caaacttaat
     2881 cgattttttt ttcgtttttt ctttaaaaat cattttcaat ggttttacct gaatctatac
     2941 agttaatgca tttgtatgtt cgttaaaatt cattgtaaat gaccttccca tatgaaggac
     3001 cgtgtattcg aataatgcca ttagtattct caacctgcag ggtccagcgg cagatcggcg
     3061 gagggaccgt tacgtctacc gtcacttcgc ctccgccgcc ctcccaatcc cagaggtcct
     3121 cgacatcgga gagttctccg agtccctcac ctactgcatc tcccgtcgtg cccaaggagt
     3181 caccctccaa gacctcccag agaccgagct cccagccgtc ctccaaccag tcgccgaggc
     3241 catggacgcc atcgccgccg ccgacctctc ccaaacctcc ggattcggac cattcggacc
     3301 acaaggaatc ggacaataca ccacctggcg tgacttcatc tgcgccatcg ccgacccaca
     3361 cgtctaccac tggcaaaccg tcatggacga caccgtctcc gcctccgtcg cccaagccct
     3421 cgacgagctc atgctctggg ccgaggactg cccagaggtc cgtcacctcg tccacgccga
     3481 cttcggatcc aacaacgtcc tcaccgacaa cggacgtatc accgccgtca tcgactggtc
     3541 cgaggccatg ttcggagact cccaatacga ggtcgccaac atcttcttct ggcgtccatg
     3601 gctcgcctgc atggagcaac aaacccgtta cttcgagcgt cgtcacccag agctcgccgg
     3661 atccccacgt ctccgtgcct acatgctccg tatcggactc gaccaactct accaatccct
     3721 cgtcgacgga aacttcgacg acgccgcctg ggcccaagga cgttgcgacg ccatcgtccg
     3781 ttccggagcc ggaaccgtcg gacgtaccca aatcgcccgt cgttccgccg ccgtctggac
     3841 cgacggatgc gtcgaggtcc tcgccgactc cggaaaccgt cgtccatcca cccgtccacg
     3901 tgccaaggag taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
     3961 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
     4021 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     4081 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     4141 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
     4201 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
     4261 tttttactag tgcaggagct gtaagtttaa aataacttcg tataggatac tttatacgaa
     4321 gttattttca gctgccctcg attgtctatc ttccattctc accaagtttc aacaacttta
     4381 ttcagtcatt cttctctggt tcatttccca accaatcttc ccgaataaag cccatctgtc
     4441 ctttcggcca cattcctcac ctacatccgt actccttatc tcccgttctt atccagtctt
     4501 ttatcgtctc tgcgtctcgc acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa
     4561 cttgaaaact tgcggaaaga tttcctaata aaaggagacg aatccggtag gaaatcgaca
     4621 agtctctgct tctcgaagac taggtgagtt acatttcttt ggccgaatta ttttgaaatc
     4681 ctaaatttga taatttcaga gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc
     4741 actgtcgcca ctcttatggt atgcttcatg accatgtcaa ccatctactc ggaggttgat
     4801 ggattcagag agaagcttga taccgagatg aatgtgttca gagtgagtta aattatattt
     4861 tgaattttaa ataattttta attttctagc aatctaccaa tggattgtgg aaggacatag
     4921 ttgtcatcgg aagatctagc aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc
     4981 caactccaca tgctgatgga tccccatctg ctccaccagg tcaaccacca gcagttccac
     5041 cagtcttcaa ccagccaaag actccaaatg gagccaatgg aaatggacca acctgcaact
     5101 gcaatgctga taacaagtgc ccagctggac catccggacc aaagggagtt ccaggagttc
     5161 caggactcga cggagttcca ggacttgacg gtgttccagg agttggagct gatgatatcg
     5221 ctccacaacg cgagtctgtc ggatgcttca cttgcccaca aggaccagtt ggaccaccag
     5281 gagctcttgg aagaccagga ccacgtggac ttccaggacc aagaggacaa aatggaaacc
     5341 caggaagaga tggacaacca ggacatccag gagagcaagg atcatccggt caaatcggaa
     5401 agatcggaga gccaggacca ccaggagaga agggacgcga cgccgagcat ccaatcggaa
     5461 gaccaggacc aaagggacca agaggagatc aaggaccaac aggaccagct ggacagaacg
     5521 gtcttcacgg accaccagga gagccaggaa ccgttggacc agaaggacca tctggaaagc
     5581 aaggacgtca aggaccagac ggaacccagg gagagactgg accagacgga agaccaggaa
     5641 aggatgccga gtactgccag tgcccagaca agtctccacc atcagaggct gtcaacgcca
     5701 accgtggata cagaaatatc taaattgttg gtgttttcta ataaaatatt tgagattcag
     5761 tgagactttt ttcttggcgg cacaataaag tttcctcttt ttgttgggtc actcaacgaa
     5821 cacaatacaa atatttgagc gctcgcattt ttctcaacct ttcgggttta aagcacttca
     5881 aaatggaatt gaagagagat tgtgaaagta catcagacag aatcacattt tgatagcatt
     5941 cacttcactc agatgcaaac ttttcagtga ctataaaaag tcataaaata ataacattaa
     6001 aaaagcaaat aaattagcga gagaattttt tgacaaaaag aaagaagagt gatagagaag
     6061 aagggaatgc ttgaaaggat tttgcattta tcttaatcac cgtcttcgag aagacgaacc
     6121 atggcgccag tttcagaatc caagtttcta atatagttca tcacaatgtt gacgttggtc
     6181 caacctccgg cttgcataat ctctgggatg gaaactccag cgcgagccat gtcacgggca
     6241 gctccgacac gggcggaatg tccagaccaa gccaagtaac gttgtccgga atcatccttg
     6301 gctccgtaga tgagacggtg ggtagcttcg aagattcctg aaaatttatt ttcagcttat
     6361 tcttagctaa tatgttgttt aaacttacct tcaagagctc gagtcgagag ttgtgatgtt
     6421 gcactcgggg cagcaactcc gttcttgcga acacgacaga acaggtagtt gtttggatca
     6481 tcggcgactc cggaaaccga aatccagcgc tcaacgagct tggtaactcc aagggagagc
     6541 gctttttcga ctccggcagt cgagaccagc gtttttgtac gtccgatgtg gatgagcatt
     6601 cttcctccgt ctgtgcgact aatatccttg acgcgaatgc gggcaatttc ggcgatacgg
     6661 agaagtgtgt tgtaagcaat tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg
     6721 gaattttcca taagagaacg gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt
     6781 aggattgaat acattaatat ttaaacttac ctttcgaagg cgagggcttg cttggctctc
     6841 tctccagcat caacattttc cttgcggata cgtctcatta cgagagatac ggcattagaa
     6901 tctgatgggc gtggaagtcc agatcggcga tggagcatat tgagctgtcc gagatgttgt
     6961 tggatcgtct tcaccgccaa tccacgagct tggaggtaga gcaagtagtc acgaacgtct
     7021 tctggctcgg ctgggaacca cttgcggttg ttaagcttgc accaggcagc ccaagaacgg
     7081 cacactgaga gaagcatctt ccaggtgtgc tcggagaagg cttgtctgtc acggaacata
     7141 tccatgaggt tcttgcgaac ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt
     7201 tgatgaacag tgagaagatt tgacatattt tcgaagtttt ttagatgcac tagaacaaag
     7261 cgtgttggct tcctctgagc ccgctttcct tatatacccg cattctgcag ccttacagaa
     7321 tgttctagaa ggtcctagat gcattcgttt gaaaatactc ccggtgggtg caaagagacg
     7381 cagacggaaa atgtatctgg gtctctttat tgtgtacact acttttccat gtaccgaatg
     7441 tgagtcgccc tccttttgca acaagcagct cgaatgttct agaaaaaggt ggaaaatagt
     7501 ataaataccg ttgaaaataa ataccgaaca acatttgctc taattgtgaa attagaaatc
     7561 ttcaaactat aatcatgtca ctttaccact atttccgtcc agctcaacgt tccgtttttg
     7621 gtgatcattt ttgctttcgt cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa
     7681 gccagcagcc aaattcgttg actgagtatt caacgtttat acgttgtcgg caacgagaaa
     7741 taggaaaatg catcgggaaa tgttcttttt tcgatttttt ccaaggtttt gacaaatttt
     7801 accacgaatt ttgctatgtt ttcaattaaa aaatatgtta ttcaactgtt tctatgagga
     7861 aaataaggct ttgcatgtaa ttttcttatt cagcataatt tttaattaat ttgaattttc
     7921 tgtcctaacg tttattttgt tttcttggtt atgactgatc tgaaattaat ttttgaattt
     7981 taaggtaata tgtcaggcgg tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag
     8041 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     8101 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     8161 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     8221 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     8281 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     8341 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     8401 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     8461 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     8521 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     8581 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     8641 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     8701 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     8761 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     8821 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     8881 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     8941 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     9001 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc
     9061 cagctttctt gtacaaagtg ggtccaatta ctcttcaaca tccctacatg ctctttctcc
     9121 ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt
     9181 ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat
     9241 tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc
     9301 aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt
     9361 gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat
     9421 gaccgcaatt tttaataact tcgtatagga tactttatac gaagttattt tcagcttaag
     9481 tttaatggtt tttcaaggaa ggaggtaatg taaatttatg ttctctccaa attgttccat
     9541 ccttctaatt atttatgtat gcagaggtat aggaaaccca gaaaaaactc cctggttggc
     9601 caagaaaaga acatatggtt tatatgcata actcggaaaa caatagcact gtagaaaagg
     9661 gtgtgtatgt atgtaataca atacagttcg ggtgtcatca aaaaggtttg gattacaatt
     9721 acaaaaggcg ggcttgtgcg ttgaaaaccg tattgctcaa gaggttttct tcatggaaat
     9781 cagaaattga attaattcaa tttgaccgag aaacggtttg agctgagcaa tccgtatgta
     9841 ctttgagaca tcactgaaaa atatttatta aacttgtgat ttggaaaatc tgaaagaaaa
     9901 agagaacatg atcaaacatc aaaaaagtga atttgtaata gataaattcc tggaggatat
     9961 ctagattttt tacagaaaaa tacaaaacat tcaaactttc ccggaacagt gtgaatttga
    10021 caggcctcag atccatttaa gccgttacgg ttagactaga ttatcaaaag tattttattt
    10081 tactttaaca aaactcaact tctacccacc gaaatttcgc tctgaagtat ttccagttca
    10141 atattcccaa ttctcgggct gtatagagaa atcgataaca tggtcatagc tgtttcctgt
    10201 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10261 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10321 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10561 taaaaa
//
