LOCUS       sequence-426567-        7680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces the Lassa virus glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Acciani M, Alston JT, Zhao G, Reynolds H, Ali AM, Xu B, Brindley MA
  TITLE     Mutational Analysis of Lassa Virus Glycoprotein Highlights Regions 
            Required for Alpha-Dystroglycan Utilization.
  JOURNAL   J Virol. 2017 Aug 24;91(18):e00574-17. doi: 10.1128/JVI.00574-17. 
            Print 2017 Sep 15.
  PUBMED    28679759
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00574-17"; journalName: "J Virol"; date: "2017-08-24- 
            24"; volume: "91"; issue: "18"; pages: "e00574-17"
COMMENT     Sequence Label: pcDNAintron-LASV-GPC
FEATURES             Location/Qualifiers
     source          1..7680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     815..839
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          939..1511
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1566..1585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1566..1584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1752..1761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3271..3288)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3277..3501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3547..3975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3634..3653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3844..3865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3984..4004)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3989..4318
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4169..4304
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4231..4250
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4385..5179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4439..4458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5049..5068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5353..5474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5390..5409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5444..5463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5523..5539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5523..5539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5536..5558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5547..5563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5571..5577,5578..5595,5596..5601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5616..5637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5754..5771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5925..6513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6005..6024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6684..7475,7476..7544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7307..7326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7545..7649)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
      841 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      901 tccgggggat cgatcccccc gatcctgaga acttcagggt gagtttgggg acccttgatt
      961 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     1021 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     1081 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     1141 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     1201 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     1261 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     1321 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     1381 tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     1441 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     1501 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     1561 aattgtaata cgactcacta tagggcgaat tcggatccac tagtccagtg tggtggaatt
     1621 ctgcagatct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg agctcatcga
     1741 tgcatggtac cgccaccatg ggtcagattg tgacattctt ccaggaagtg cctcatgtga
     1801 ttgaagaggt gatgaacatt gtgctcattg ccctgtccgt gctcgccgtg ctgaaaggtc
     1861 tgtacaattt tgccacctgt ggcctggtgg gtctggtgac attcctcctg ctgtgtggta
     1921 ggtcctgcac aaccagcctc tacaaaggtg tgtacgagct ccagacactg gaactgaaca
     1981 tggagacact caatatgacc atgcctctct cctgcacaaa gaacaacagc catcattaca
     2041 tcatggtggg caatgagaca ggcctggaac tgaccctgac caacacaagc attattaatc
     2101 acaaattttg caatctgtcc gatgcccaca aaaagaacct ctacgaccac gccctcatga
     2161 gcattatctc caccttccac ctgtccatcc ccaacttcaa tcagtacgag gccatgagct
     2221 gcgattttaa tggcggtaag attagcgtgc agtacaacct gagccacagc tacgccggcg
     2281 atgccgccaa ccattgtggt acagtggcca atggtgtgct gcagaccttt atgaggatgg
     2341 cctggggtgg tagctacatt gccctcgact ccggcagggg caactgggac tgtattatga
     2401 ccagctacca gtacctgatt atccagaata caacctggga agatcactgc cagttctcca
     2461 gaccatcccc catcggttac ctcggtctcc tctcccagag gaccagagat atttacatta
     2521 gcagaagact gctcggcaca ttcacatgga cactgtccga ttccgaaggt aaagacacac
     2581 caggtggcta ctgtctgacc aggtggatgc tcattgaggc cgaactcaaa tgcttcggta
     2641 acacagccgt ggccaaatgt aatgagaagc atgatgagga attttgtgac atgctgaggc
     2701 tgtttgactt caacaaacag gccattcaga ggctgaaagc cgaagcccag atgagcattc
     2761 agctgatcaa caaagccgtg aacgccctga ttaatgacca gctcatcatg aagaaccatc
     2821 tgagggacat catgggcatt ccatactgta attacagcaa gtactggtat ctcaaccaca
     2881 caaccacagg tagaacatcc ctgcccaaat gttggctcgt gtccaatggt tcctacctga
     2941 acgagaccca cttttccgat gatattgaac agcaggccga caatatgatc accgagatgc
     3001 tgcagaagga gtacatggag aggcagggta agacaccact gggtctggtg gacctctttg
     3061 tgttcagcac aagcttctac ctcattagca tcttcctcca cctggtgaaa attccaacac
     3121 ataggcatat tgtgggcaag tcctgtccca aacctcacag actgaatcat atgggcattt
     3181 gttcctgtgg tctctacaaa cagcctggtg tgcctgtgaa atggaagaga tgactcgagt
     3241 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3301 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3361 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3421 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3481 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     3541 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3601 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3661 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3721 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3781 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3841 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3961 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4021 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4081 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4141 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4201 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     4261 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4321 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     4381 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4441 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4501 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4561 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4621 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4681 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4741 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4801 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4861 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     4921 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4981 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5041 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5101 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5161 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5221 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5281 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5341 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5461 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     5521 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5581 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5701 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6121 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6961 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
