LOCUS       sequence-426573-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces the Chikungunya virus envelope protein E3-E2-6K-E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Reyes Ballista JM, Hoover AJ, Noble JT, Acciani MD, Miazgowicz KL, 
            Harrison SA, Tabscott GAL, Duncan A, Barnes DN, Jimenez AR, Brindley
            MA
  TITLE     Chikungunya virus release is reduced by TIM-1 receptors through 
            binding of envelope phosphatidylserine.
  JOURNAL   J Virol. 2024 Jul 15:e0077524. doi: 10.1128/jvi.00775-24.
  PUBMED    39007616
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2024 Jul 15:e0077524. doi: 10.1128/jvi.00775-24."
COMMENT     Sequence Label: pcDNAintron-CHIKV-Envelope protein
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     325..344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        325..343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3521..3538)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3527..3751
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3797..4225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3884..3903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4094..4115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4234..4254)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4239..4568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4419..4554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4481..4500
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4635..5429
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4689..4708)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5299..5318
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5603..5724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5640..5659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5694..5713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5773..5789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5773..5789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5786..5808)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5797..5813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5821..5827,5828..5845,5846..5851))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5866..5887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6004..6021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6175..6763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6255..6274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6934..7725,7726..7794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7557..7576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7795..7899)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7974..7993)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8165..8544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8545..8748
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8699..8719
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8745..8769
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(8869..9171,1..270)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
       61 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
      121 attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga
      181 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
      241 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
      301 tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt cggatccact
      361 agtccagtgt ggtggaattc tgcagatcta gagcctctgc taaccatgtt catgccttct
      421 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
      481 aagaattcga gctcatcgat gcatggtacc gccaccatga gtcttgccat tccagttatg
      541 tgcctgctgg caaataccac gttcccctgc tcccagcccc cttgcacacc ctgctgctac
      601 gaaaaagagc cggagaaaac cctgcgcatg ctagaagaca acgtcatgag ccccgggtac
      661 tatcagctgc tacaagcatc cttaacatgt tctccccgcc gccagcgacg cagtattaag
      721 gacaacttca atgtctataa agccataaga ccgtacctag ctcactgtcc cgactgtgga
      781 gaagggcact cgtgccatag tcccgtagcg ctagaacgca tcagaaacga agcgacagac
      841 gggacgctga aaatccaggt ttccttgcaa atcggaataa agacggatga tagccatgat
      901 tggaccaagc tgcgttacat ggacaatcat atgccagcag acgcagagag ggccaggcta
      961 tttgtaagaa cgtcagcacc gtgcacgatt actggaacaa tgggacactt catcctggcc
     1021 cgatgtccga aaggagaaac tctgacggtg ggattcactg acggtaggaa gatcagtcac
     1081 tcatgtacgc acccatttca ccacgaccct cctgtgatag gccgggaaaa atttcattcc
     1141 cgaccgcagc acggtagaga actaccttgc agcacgtacg cgcagagcac cgctgcaact
     1201 gccgaggaga tagaggtaca tatgccccca gacaccccag atcgcacatt gatgtcacaa
     1261 cagtccggta atgtaaagat cacagtcaat agtcagacgg tgcggtacaa gtgtaattgc
     1321 ggtgactcaa atgaaggact aaccactaca gacaaagtga ttaataactg caaggttgat
     1381 caatgccatg ccgcggtcac caatcacaaa aaatggcagt ataattcccc tctggtcccg
     1441 cgtaatgctg aactcgggga ccgaaaagga aaagttcaca ttccgtttcc tctggcaaat
     1501 gtgacatgca gggtgcctaa ggcaaggaac cccaccgtga cgtacggaaa aaaccaagtc
     1561 atcatgctgc tgtatcctga ccacccaacg ctcctgtcct accggaatat gggagaagaa
     1621 ccaaactatc aagaagagtg ggtgacgcat aagaaggaga tcaggttaac cgtgccgact
     1681 gaagggctcg aggtcacgtg gggcaacaac gagccgtaca agtattggcc gcagttatcc
     1741 acaaacggta cagcccacgg ccacccgcat gagataattt tgtattatta tgagctgtac
     1801 cctactatga ctgtggtagt tgtgtcagtg gcctcgttcg tactcctgtc gatggtgggt
     1861 gtggcagtgg ggatgtgcat gtgtgcacga cgcagatgca ttacaccgta cgaactgaca
     1921 ccaggagcta ccgtcccttt cctgcttagc ctaatatgct gcattagaac agctaaagcg
     1981 gccacatacc aagaggctgc ggtatacctg tggaacgagc agcagccttt gttttggctg
     2041 caagccctta ttccgctggc agccctgatt gtcctatgca actgtctgag actcttacca
     2101 tgcttttgta aaacgttgac ttttttagcc gtaatgagcg tcggtgccca cactgtgagc
     2161 gcgtacgaac acgtaacagt gatcccgaac acggtgggag taccgtataa gactctagtc
     2221 aacagaccgg gctacagccc catggtactg gagatggagc ttctgtcagt cactttggag
     2281 ccaacgctat cgcttgatta catcacgtgc gagtataaaa ccgtcatccc gtctccgtac
     2341 gtgaaatgct gcggtacagc agagtgcaag gacaagagcc tacctgatta cagctgtaag
     2401 gtcttcaccg gcgtctaccc attcatgtgg ggcggcgcct actgcttctg cgacactgaa
     2461 aatacgcaat tgagcgaagc acatgtggag aagtccgaat catgcaaaac agaatttgca
     2521 tcagcatata gggctcatac cgcatccgca tcagctaagc tccgcgtcct ttaccaagga
     2581 aataatgtta ctgtatctgc ttatgcaaac ggcgatcatg ccgtcacagt taaggacgct
     2641 aaattcattg tggggccaat gtcttcagcc tggacacctt ttgacaataa aatcgtggtg
     2701 tacaaaggcg acgtctacaa catggactac ccgcccttcg gcgcaggaag accaggacaa
     2761 tttggcgaca tccaaagtcg cacgcctgag agcgaagacg tctatgctaa cacacaactg
     2821 gtactgcaga gaccgtccgc gggtacggtg cacgtgccgt actctcaggc accatctggc
     2881 ttcaagtatt ggctaaaaga acgaggggcg tcgctgcagc acacagcacc atttggctgt
     2941 caaatagcaa caaacccggt aagagcgatg aactgcgccg tagggaacat gcctatctcc
     3001 atcgacatac cggacgcggc cttcactagg gtcgtcgacg cgccatcttt aacggacatg
     3061 tcgtgtgagg taccagcctg cacccactcc tcagactttg ggggcgtagc catcattaaa
     3121 tatgcagcca gcaagaaagg caagtgtgcg gtgcattcga tgactaacgc cgtcactatt
     3181 cgggaagctg aaatagaagt agaagggaac tctcagttgc aaatctcttt ttcgacggcc
     3241 ctagccagcg ccgaattccg cgtacaagtc tgttctacac aagtacactg tgcagccgag
     3301 tgccatccac cgaaagacca tatagtcaat tacccggcgt cacacaccac cctcggggtc
     3361 caagacattt ccgttacggc gatgtcatgg gtgcagaaga tcacgggagg tgtgggactg
     3421 gttgtcgctg ttgcagcact gatcctaatc gtggtgctat gcgtgtcgtt tagcaggcac
     3481 tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3541 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3601 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3661 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3721 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3781 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4021 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4081 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4141 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4201 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4321 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4381 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4441 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4501 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4561 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4621 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4681 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4741 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4801 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4861 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4921 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4981 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5041 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5101 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5161 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5221 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5281 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5341 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5401 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5461 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5521 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5581 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5701 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5761 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5821 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5881 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5941 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6001 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6061 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6601 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7321 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7921 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     7981 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8041 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     8101 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     8161 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     8221 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     8281 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     8341 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     8401 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     8461 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     8521 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     8581 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     8641 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     8701 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     8761 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     8821 cgatccagcc tccgggggat cgatcccccc gatcctgaga acttcagggt gagtttgggg
     8881 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
     8941 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
     9001 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
     9061 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     9121 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt t
//
