LOCUS       sequence-426574-        9951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces the Chikungunya virus structural polyprotein 
            Capsid-E3-E2-6K-E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Reyes Ballista JM, Hoover AJ, Noble JT, Acciani MD, Miazgowicz KL, 
            Harrison SA, Tabscott GAL, Duncan A, Barnes DN, Jimenez AR, Brindley
            MA
  TITLE     Chikungunya virus release is reduced by TIM-1 receptors through 
            binding of envelope phosphatidylserine.
  JOURNAL   J Virol. 2024 Jul 15:e0077524. doi: 10.1128/jvi.00775-24.
  PUBMED    39007616
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2024 Jul 15:e0077524. doi: 10.1128/jvi.00775-24."
COMMENT     Sequence Label: pcDNAintron-CHIKV-Structural polyprotein
FEATURES             Location/Qualifiers
     source          1..9951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     325..344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        325..343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      511..520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4301..4318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4307..4531
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4577..5005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4664..4683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4874..4895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5014..5034)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5019..5348
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5199..5334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5261..5280
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5415..6209
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5469..5488)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6079..6098
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6383..6504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6420..6439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6474..6493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6553..6569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6553..6569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6566..6588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6577..6593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6601..6607,6608..6625,6626..6631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6646..6667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6784..6801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6955..7543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7035..7054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7714..8505,8506..8574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8337..8356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8575..8679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8754..8773)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8945..9324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9325..9528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9479..9499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9525..9549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(9649..9951,1..270)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
       61 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
      121 attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga
      181 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
      241 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
      301 tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt cggatccact
      361 agtccagtgt ggtggaattc tgcagatcta gagcctctgc taaccatgtt catgccttct
      421 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
      481 aagaattcga gctcatcgat gcatggtacc gccaccatgg agtttatccc aacccaaact
      541 ttctacaata ggaggtacca gcctcgacct tggactccgc gccctactat ccaagttatc
      601 agacccagac cgcgtccgca aaggaaagcc gggcaacttg cccagctgat ctcagcagtt
      661 aataaactga caatgcgcgc ggtacctcaa cagaagccgc gcaagaatcg gaagaataag
      721 aagcaaaagc aaaagcagca ggcgccacga aacaacatga atcaaaagaa gcagccccct
      781 aaaaagaaac cggctcaaaa gaaaaagaag ccgggccgta gagagagaat gtgcatgaaa
      841 atcgaaaatg attgcatctt cgaagtcaag catgaaggta aggtaacagg ttacgcgtgc
      901 ttggtagggg acaaagtaat gaagccagca cacgtaaagg ggaccatcga taatgcggac
      961 ctggccaaat tggccttcaa gcggtcatct aagtacgacc ttgaatgcgc gcagataccc
     1021 gtgcacatga agtccgacgc ttcgaagttc acccatgaga aaccggaggg gtactacaac
     1081 tggcaccacg gagcagtaca gtactcagga ggccggttca ccatccctac aggtgcgggc
     1141 aaaccagggg acagcggtag accgatcttc gacaacaagg ggcgcgtggt ggccatagtt
     1201 ttaggaggag ctaatgaagg agcccgtaca gccctctcgg tggtgacctg gaacaaagac
     1261 atcgtcacga aaatcacccc tgagggggcc gaagagtgga gtcttgccat tccagttatg
     1321 tgcctgctgg caaataccac gttcccctgc tcccagcccc cttgcacacc ctgctgctac
     1381 gaaaaagagc cggagaaaac cctgcgcatg ctagaagaca acgtcatgag ccccgggtac
     1441 tatcagctgc tacaagcatc cttaacatgt tctccccgcc gccagcgacg cagtattaag
     1501 gacaacttca atgtctataa agccataaga ccgtacctag ctcactgtcc cgactgtgga
     1561 gaagggcact cgtgccatag tcccgtagcg ctagaacgca tcagaaacga agcgacagac
     1621 gggacgctga aaatccaggt ttccttgcaa atcggaataa agacggatga tagccatgat
     1681 tggaccaagc tgcgttacat ggacaatcat atgccagcag acgcagagag ggccaggcta
     1741 tttgtaagaa cgtcagcacc gtgcacgatt actggaacaa tgggacactt catcctggcc
     1801 cgatgtccga aaggagaaac tctgacggtg ggattcactg acggtaggaa gatcagtcac
     1861 tcatgtacgc acccatttca ccacgaccct cctgtgatag gccgggaaaa atttcattcc
     1921 cgaccgcagc acggtagaga actaccttgc agcacgtacg cgcagagcac cgctgcaact
     1981 gccgaggaga tagaggtaca tatgccccca gacaccccag atcgcacatt gatgtcacaa
     2041 cagtccggta atgtaaagat cacagtcaat agtcagacgg tgcggtacaa gtgtaattgc
     2101 ggtgactcaa atgaaggact aaccactaca gacaaagtga ttaataactg caaggttgat
     2161 caatgccatg ccgcggtcac caatcacaaa aaatggcagt ataattcccc tctggtcccg
     2221 cgtaatgctg aactcgggga ccgaaaagga aaagttcaca ttccgtttcc tctggcaaat
     2281 gtgacatgca gggtgcctaa ggcaaggaac cccaccgtga cgtacggaaa aaaccaagtc
     2341 atcatgctgc tgtatcctga ccacccaacg ctcctgtcct accggaatat gggagaagaa
     2401 ccaaactatc aagaagagtg ggtgacgcat aagaaggaga tcaggttaac cgtgccgact
     2461 gaagggctcg aggtcacgtg gggcaacaac gagccgtaca agtattggcc gcagttatcc
     2521 acaaacggta cagcccacgg ccacccgcat gagataattt tgtattatta tgagctgtac
     2581 cctactatga ctgtggtagt tgtgtcagtg gcctcgttcg tactcctgtc gatggtgggt
     2641 gtggcagtgg ggatgtgcat gtgtgcacga cgcagatgca ttacaccgta cgaactgaca
     2701 ccaggagcta ccgtcccttt cctgcttagc ctaatatgct gcattagaac agctaaagcg
     2761 gccacatacc aagaggctgc ggtatacctg tggaacgagc agcagccttt gttttggctg
     2821 caagccctta ttccgctggc agccctgatt gtcctatgca actgtctgag actcttacca
     2881 tgcttttgta aaacgttgac ttttttagcc gtaatgagcg tcggtgccca cactgtgagc
     2941 gcgtacgaac acgtaacagt gatcccgaac acggtgggag taccgtataa gactctagtc
     3001 aacagaccgg gctacagccc catggtactg gagatggagc ttctgtcagt cactttggag
     3061 ccaacgctat cgcttgatta catcacgtgc gagtataaaa ccgtcatccc gtctccgtac
     3121 gtgaaatgct gcggtacagc agagtgcaag gacaagagcc tacctgatta cagctgtaag
     3181 gtcttcaccg gcgtctaccc attcatgtgg ggcggcgcct actgcttctg cgacactgaa
     3241 aatacgcaat tgagcgaagc acatgtggag aagtccgaat catgcaaaac agaatttgca
     3301 tcagcatata gggctcatac cgcatccgca tcagctaagc tccgcgtcct ttaccaagga
     3361 aataatgtta ctgtatctgc ttatgcaaac ggcgatcatg ccgtcacagt taaggacgct
     3421 aaattcattg tggggccaat gtcttcagcc tggacacctt ttgacaataa aatcgtggtg
     3481 tacaaaggcg acgtctacaa catggactac ccgcccttcg gcgcaggaag accaggacaa
     3541 tttggcgaca tccaaagtcg cacgcctgag agcgaagacg tctatgctaa cacacaactg
     3601 gtactgcaga gaccgtccgc gggtacggtg cacgtgccgt actctcaggc accatctggc
     3661 ttcaagtatt ggctaaaaga acgaggggcg tcgctgcagc acacagcacc atttggctgt
     3721 caaatagcaa caaacccggt aagagcgatg aactgcgccg tagggaacat gcctatctcc
     3781 atcgacatac cggacgcggc cttcactagg gtcgtcgacg cgccatcttt aacggacatg
     3841 tcgtgtgagg taccagcctg cacccactcc tcagactttg ggggcgtagc catcattaaa
     3901 tatgcagcca gcaagaaagg caagtgtgcg gtgcattcga tgactaacgc cgtcactatt
     3961 cgggaagctg aaatagaagt agaagggaac tctcagttgc aaatctcttt ttcgacggcc
     4021 ctagccagcg ccgaattccg cgtacaagtc tgttctacac aagtacactg tgcagccgag
     4081 tgccatccac cgaaagacca tatagtcaat tacccggcgt cacacaccac cctcggggtc
     4141 caagacattt ccgttacggc gatgtcatgg gtgcagaaga tcacgggagg tgtgggactg
     4201 gttgtcgctg ttgcagcact gatcctaatc gtggtgctat gcgtgtcgtt tagcaggcac
     4261 tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4321 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4381 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4441 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4501 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4561 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4621 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4681 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4741 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4801 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4861 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4921 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4981 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     5041 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5101 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5161 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5221 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5281 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5341 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     5401 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5461 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     5521 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5581 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5641 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5701 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5761 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5821 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5881 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5941 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6001 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6061 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6121 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6181 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     6241 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6301 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6361 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6481 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7381 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8701 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     8761 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8821 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     8881 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     8941 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     9001 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9061 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     9121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     9241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     9301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     9361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     9421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     9481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     9541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     9601 cgatccagcc tccgggggat cgatcccccc gatcctgaga acttcagggt gagtttgggg
     9661 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
     9721 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
     9781 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
     9841 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     9901 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt t
//
