LOCUS       sequence-426577-       10464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces the Chikungunya virus structural polyprotein 
            Capsid-E3-NLucE2-6K-E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Reyes Ballista JM, Hoover AJ, Noble JT, Acciani MD, Miazgowicz KL, 
            Harrison SA, Tabscott GAL, Duncan A, Barnes DN, Jimenez AR, Brindley
            MA
  TITLE     Chikungunya virus release is reduced by TIM-1 receptors through 
            binding of envelope phosphatidylserine.
  JOURNAL   J Virol. 2024 Jul 15:e0077524. doi: 10.1128/jvi.00775-24.
  PUBMED    39007616
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2024 Jul 15:e0077524. doi: 10.1128/jvi.00775-24."
COMMENT     Sequence Label: pcDNAintron-CHIKV-Structural polyprotein with ...
FEATURES             Location/Qualifiers
     source          1..10464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     325..344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        325..343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      511..520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1489..2001
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(4814..4831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4820..5044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5090..5518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5177..5196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5387..5408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5527..5547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5532..5861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5712..5847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5774..5793
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5928..6722
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5982..6001)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6592..6611
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6896..7017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6933..6952)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6987..7006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7066..7082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7066..7082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7079..7101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7090..7106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7114..7120,7121..7138,7139..7144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7159..7180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7297..7314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7468..8056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7548..7567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8227..9018,9019..9087))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8850..8869
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9088..9192)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9267..9286)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9458..9837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9838..10041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9992..10012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10038..10062
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(10162..10464,1..270)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
       61 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
      121 attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga
      181 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
      241 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
      301 tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt cggatccact
      361 agtccagtgt ggtggaattc tgcagatcta gagcctctgc taaccatgtt catgccttct
      421 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
      481 aagaattcga gctcatcgat gcatggtacc gccaccatgg agtttatccc aacccaaact
      541 ttctacaata ggaggtacca gcctcgacct tggactccgc gccctactat ccaagttatc
      601 agacccagac cgcgtccgca aaggaaagcc gggcaacttg cccagctgat ctcagcagtt
      661 aataaactga caatgcgcgc ggtacctcaa cagaagccgc gcaagaatcg gaagaataag
      721 aagcaaaagc aaaagcagca ggcgccacga aacaacatga atcaaaagaa gcagccccct
      781 aaaaagaaac cggctcaaaa gaaaaagaag ccgggccgta gagagagaat gtgcatgaaa
      841 atcgaaaatg attgcatctt cgaagtcaag catgaaggta aggtaacagg ttacgcgtgc
      901 ttggtagggg acaaagtaat gaagccagca cacgtaaagg ggaccatcga taatgcggac
      961 ctggccaaat tggccttcaa gcggtcatct aagtacgacc ttgaatgcgc gcagataccc
     1021 gtgcacatga agtccgacgc ttcgaagttc acccatgaga aaccggaggg gtactacaac
     1081 tggcaccacg gagcagtaca gtactcagga ggccggttca ccatccctac aggtgcgggc
     1141 aaaccagggg acagcggtag accgatcttc gacaacaagg ggcgcgtggt ggccatagtt
     1201 ttaggaggag ctaatgaagg agcccgtaca gccctctcgg tggtgacctg gaacaaagac
     1261 atcgtcacga aaatcacccc tgagggggcc gaagagtgga gtcttgccat tccagttatg
     1321 tgcctgctgg caaataccac gttcccctgc tcccagcccc cttgcacacc ctgctgctac
     1381 gaaaaagagc cggagaaaac cctgcgcatg ctagaagaca acgtcatgag ccccgggtac
     1441 tatcagctgc tacaagcatc cttaacatgt tctccccgcc gccagcgaat ggtcttcaca
     1501 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     1561 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     1621 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     1681 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     1741 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     1801 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     1861 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     1921 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     1981 ctgtgcgaac gcattctggc gcgcagtatt aaggacaact tcaatgtcta taaagccata
     2041 agaccgtacc tagctcactg tcccgactgt ggagaagggc actcgtgcca tagtcccgta
     2101 gcgctagaac gcatcagaaa cgaagcgaca gacgggacgc tgaaaatcca ggtttccttg
     2161 caaatcggaa taaagacgga tgatagccat gattggacca agctgcgtta catggacaat
     2221 catatgccag cagacgcaga gagggccagg ctatttgtaa gaacgtcagc accgtgcacg
     2281 attactggaa caatgggaca cttcatcctg gcccgatgtc cgaaaggaga aactctgacg
     2341 gtgggattca ctgacggtag gaagatcagt cactcatgta cgcacccatt tcaccacgac
     2401 cctcctgtga taggccggga aaaatttcat tcccgaccgc agcacggtag agaactacct
     2461 tgcagcacgt acgcgcagag caccgctgca actgccgagg agatagaggt acatatgccc
     2521 ccagacaccc cagatcgcac attgatgtca caacagtccg gtaatgtaaa gatcacagtc
     2581 aatagtcaga cggtgcggta caagtgtaat tgcggtgact caaatgaagg actaaccact
     2641 acagacaaag tgattaataa ctgcaaggtt gatcaatgcc atgccgcggt caccaatcac
     2701 aaaaaatggc agtataattc ccctctggtc ccgcgtaatg ctgaactcgg ggaccgaaaa
     2761 ggaaaagttc acattccgtt tcctctggca aatgtgacat gcagggtgcc taaggcaagg
     2821 aaccccaccg tgacgtacgg aaaaaaccaa gtcatcatgc tgctgtatcc tgaccaccca
     2881 acgctcctgt cctaccggaa tatgggagaa gaaccaaact atcaagaaga gtgggtgacg
     2941 cataagaagg agatcaggtt aaccgtgccg actgaagggc tcgaggtcac gtggggcaac
     3001 aacgagccgt acaagtattg gccgcagtta tccacaaacg gtacagccca cggccacccg
     3061 catgagataa ttttgtatta ttatgagctg taccctacta tgactgtggt agttgtgtca
     3121 gtggcctcgt tcgtactcct gtcgatggtg ggtgtggcag tggggatgtg catgtgtgca
     3181 cgacgcagat gcattacacc gtacgaactg acaccaggag ctaccgtccc tttcctgctt
     3241 agcctaatat gctgcattag aacagctaaa gcggccacat accaagaggc tgcggtatac
     3301 ctgtggaacg agcagcagcc tttgttttgg ctgcaagccc ttattccgct ggcagccctg
     3361 attgtcctat gcaactgtct gagactctta ccatgctttt gtaaaacgtt gactttttta
     3421 gccgtaatga gcgtcggtgc ccacactgtg agcgcgtacg aacacgtaac agtgatcccg
     3481 aacacggtgg gagtaccgta taagactcta gtcaacagac cgggctacag ccccatggta
     3541 ctggagatgg agcttctgtc agtcactttg gagccaacgc tatcgcttga ttacatcacg
     3601 tgcgagtata aaaccgtcat cccgtctccg tacgtgaaat gctgcggtac agcagagtgc
     3661 aaggacaaga gcctacctga ttacagctgt aaggtcttca ccggcgtcta cccattcatg
     3721 tggggcggcg cctactgctt ctgcgacact gaaaatacgc aattgagcga agcacatgtg
     3781 gagaagtccg aatcatgcaa aacagaattt gcatcagcat atagggctca taccgcatcc
     3841 gcatcagcta agctccgcgt cctttaccaa ggaaataatg ttactgtatc tgcttatgca
     3901 aacggcgatc atgccgtcac agttaaggac gctaaattca ttgtggggcc aatgtcttca
     3961 gcctggacac cttttgacaa taaaatcgtg gtgtacaaag gcgacgtcta caacatggac
     4021 tacccgccct tcggcgcagg aagaccagga caatttggcg acatccaaag tcgcacgcct
     4081 gagagcgaag acgtctatgc taacacacaa ctggtactgc agagaccgtc cgcgggtacg
     4141 gtgcacgtgc cgtactctca ggcaccatct ggcttcaagt attggctaaa agaacgaggg
     4201 gcgtcgctgc agcacacagc accatttggc tgtcaaatag caacaaaccc ggtaagagcg
     4261 atgaactgcg ccgtagggaa catgcctatc tccatcgaca taccggacgc ggccttcact
     4321 agggtcgtcg acgcgccatc tttaacggac atgtcgtgtg aggtaccagc ctgcacccac
     4381 tcctcagact ttgggggcgt agccatcatt aaatatgcag ccagcaagaa aggcaagtgt
     4441 gcggtgcatt cgatgactaa cgccgtcact attcgggaag ctgaaataga agtagaaggg
     4501 aactctcagt tgcaaatctc tttttcgacg gccctagcca gcgccgaatt ccgcgtacaa
     4561 gtctgttcta cacaagtaca ctgtgcagcc gagtgccatc caccgaaaga ccatatagtc
     4621 aattacccgg cgtcacacac caccctcggg gtccaagaca tttccgttac ggcgatgtca
     4681 tgggtgcaga agatcacggg aggtgtggga ctggttgtcg ctgttgcagc actgatccta
     4741 atcgtggtgc tatgcgtgtc gtttagcagg cactgactcg agtctagagg gcccgtttaa
     4801 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4861 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4921 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4981 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5041 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5101 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5161 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5221 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5281 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5341 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5401 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5461 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5521 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5581 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5641 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5701 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5761 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5821 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5881 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5941 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6001 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6061 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6121 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6181 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6241 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6301 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6361 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6421 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6481 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6541 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6601 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6661 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6721 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6781 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6841 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6901 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6961 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7021 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7081 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7141 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7201 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7261 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8221 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8281 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8341 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8401 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8461 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8521 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8581 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8641 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8701 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8761 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8821 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8881 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8941 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9001 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9061 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9121 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9181 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     9241 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9301 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     9361 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     9421 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     9481 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     9541 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     9601 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     9661 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     9721 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     9781 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     9841 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     9901 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     9961 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    10021 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
    10081 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcgatccc
    10141 cccgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
    10201 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
    10261 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
    10321 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
    10381 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
    10441 tactttctct aatcactttt tttt
//
