LOCUS       sequence-426581-        8230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces the Ebola virus glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Acciani MD, Lay Mendoza MF, Havranek KE, Duncan AM, Iyer H, Linn OL,
            Brindley MA
  TITLE     Ebola Virus Requires Phosphatidylserine Scrambling Activity for 
            Efficient Budding and Optimal Infectivity.
  JOURNAL   J Virol. 2021 Sep 27;95(20):e0116521. doi: 10.1128/JVI.01165-21. 
            Epub 2021 Jul 28.
  PUBMED    34319156
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-09-27"; volume: "95(20):e0116521. doi"; pages: " 
            10.1128/JVI.01165-21. Epub 2021 Jul 28"
COMMENT     Sequence Label: pcDNAintron-EBOV-GP
FEATURES             Location/Qualifiers
     source          1..8230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     585..609
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          709..1281
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1336..1355
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1336..1354
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1522..1531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3591..3608)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3597..3821
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3867..4295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3954..3973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4164..4185
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4304..4324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4309..4638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4489..4624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4551..4570
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4705..5499
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4759..4778)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5369..5388
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5673..5794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5764..5783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5843..5859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5843..5859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5856..5878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5867..5883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5891..5897,5898..5915,5916..5921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5936..5957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6074..6091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6245..6833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6325..6344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7004..7795,7796..7864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7627..7646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7865..7969)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8044..8063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      601 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      661 cgatccagcc tccgggggat cgatcccccc gatcctgaga acttcagggt gagtttgggg
      721 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
      781 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
      841 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
      901 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
      961 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
     1021 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
     1081 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
     1141 aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
     1201 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
     1261 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     1321 tttggcaaag aattgtaata cgactcacta tagggcgaat tcggatccac tagtccagtg
     1381 tggtggaatt ctgcagatct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     1441 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg
     1501 agctcatcga tgcatggtac cgccaccatg ggcgttacag gaatattgca gttacctcgt
     1561 gatcgattca agaggacatc attctttctt tgggtaatta tccttttcca aagaacattt
     1621 tccatcccac ttggagtcat ccacaatagc acattacagg ttagtgatgt cgacaaacta
     1681 gtttgtcgtg acaaactgtc atccacaaat caattgagat cagttggact gaatctcgaa
     1741 gggaatggag tggcaactga cgtgccatct gcaactaaaa gatggggctt caggtccggt
     1801 gtcccaccaa aggtggtcaa ttatgaagct ggtgaatggg ctgaaaactg ctacaatctt
     1861 gaaatcaaaa aacctgacgg gagtgagtgt ctaccagcag cgccagacgg gattcggggc
     1921 ttcccccggt gccggtatgt gcacaaagta tcaggaacgg gaccgtgtgc cggagacttt
     1981 gccttccata aagagggtgc tttcttcctg tatgatcgac ttgcttccac agttatctac
     2041 cgaggaacga ctttcgctga aggtgtcgtt gcatttctga tactgcccca agctaagaag
     2101 gacttcttca gctcacaccc cttgagagag ccggtcaatg caacggagga cccgtctagt
     2161 ggctactatt ctaccacaat tagatatcag gctaccggtt ttggaaccaa tgagacagag
     2221 tacttgttcg aggttgacaa tttgacctac gtccaacttg aatcaagatt cacaccacag
     2281 tttctgctcc agctgaatga gacaatatat acaagtggga aaaggagcaa taccacggga
     2341 aaactaattt ggaaggtcaa ccccgaaatt gatacaacaa tcggggagtg ggccttctgg
     2401 gaaactaaaa aaaacctcac tagaaaaatt cgcagtgaag agttgtcttt cacagttgta
     2461 tcaaacggag ccaaaaacat cagtggtcag agtccggcgc gaacttcttc cgacccaggg
     2521 accaacacaa caactgaaga ccacaaaatc atggcttcag aaaattcctc tgcaatggtt
     2581 caagtgcaca gtcaaggaag ggaagctgca gtgtcgcatc taacaaccct tgccacaatc
     2641 tccacgagtc cccaatccct cacaaccaaa ccaggtccgg acaacagcac ccataataca
     2701 cccgtgtata aacttgacat ctctgaggca actcaagttg aacaacatca ccgcagaaca
     2761 gacaacgaca gcacagcctc cgacactccc tctgccacga ccgcagccgg acccccaaaa
     2821 gcagagaaca ccaacacgag caagagcact gacttcctgg accccgccac cacaacaagt
     2881 ccccaaaacc acagcgagac cgctggcaac aacaacactc atcaccaaga taccggagaa
     2941 gagagtgcca gcagcgggaa gctaggctta attaccaata ctattgctgg agtcgcagga
     3001 ctgatcacag gcgggagaag aactcgaaga gaagcaattg tcaatgctca acccaaatgc
     3061 aaccctaatt tacattactg gactactcag gatgaaggtg ctgcaatcgg actggcctgg
     3121 ataccatatt tcgggccagc agccgaggga atttacatag aggggctaat gcacaatcaa
     3181 gatggtttaa tctgtgggtt gagacagctg gccaacgaga cgactcaagc tcttcaactg
     3241 ttcctgagag ccacaactga gctacgcacc ttttcaatcc tcaaccgtaa ggcaattgat
     3301 ttcttgctgc agcgatgggg cggcacatgc cacattctgg gaccggactg ctgtatcgaa
     3361 ccacatgatt ggaccaagaa cataacagac aaaattgatc agattattca tgattttgtt
     3421 gataaaaccc ttccggacca gggggacaat gacaattggt ggacaggatg gagacaatgg
     3481 ataccggcag gtattggagt tacaggcgtt gtaattgcag ttatcgcttt attctgtata
     3541 tgcaaatttg tcttttagct cgagagggcc cgtttaaacc cgctgatcag cctcgactgt
     3601 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3661 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3721 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3781 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3841 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3901 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3961 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4021 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4081 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4141 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4201 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4261 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4561 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4621 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     4681 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4741 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4801 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4861 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4921 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4981 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5041 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5101 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5161 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5221 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5281 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5341 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5401 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5461 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5521 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5581 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5641 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5821 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7981 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8041 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8101 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8161 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8221 cagatatacg
//
