LOCUS       sequence-42659-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Stelnicki EJ, Arbeit J, Cass DL, Saner C, Harrison M, Largman C
  TITLE     Modulation of the human homeobox genes PRX-2 and HOXB13 in scarless 
            fetal wounds.
  JOURNAL   J Invest Dermatol 1998 Jul;111(1):57-63.
  PUBMED    9665387
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Invest 
            Dermatol"; date: "1998-07"; volume: "111(1)"; pages: "57-63"
COMMENT     Sequence Label: HOXB13 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcgcatgg agcccggcaa ttatgccacc ttggatggag ccaaggatat cgaaggcttg
      181 ctgggagcgg gaggggggcg gaatctggtc gcccactccc ctctgaccag ccacccagcg
      241 gcgcctacgc tgatgcctgc tgtcaactat gcccccttgg atctgccagg ctcggcggag
      301 ccgccaaagc aatgccaccc atgccctggg gtgccccagg ggacgtcccc agctcccgtg
      361 ccttatggtt actttggagg cgggtactac tcctgccgag tgtcccggag ctcgctgaaa
      421 ccctgtgccc aggcagccac cctggccgcg taccccgcgg agactcccac ggccggggaa
      481 gagtacccca gtcgccccac tgagtttgcc ttctatccgg gatatccggg aacctaccag
      541 cctatggcca gttacctgga cgtgtctgtg gtgcagactc tgggtgctcc tggagaaccg
      601 cgacatgact ccctgttgcc tgtggacagt taccagtctt gggctctcgc tggtggctgg
      661 aacagccaga tgtgttgcca gggagaacag aacccaccag gtcccttttg gaaggcagca
      721 tttgcagact ccagcgggca gcaccctcct gacgcctgcg cctttcgtcg cggcc
//
