LOCUS       sequence-42662-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     pHyVec Cloning Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHyVec4
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(524..544)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(524..542)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(563..579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(576..598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    587..603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(611..617,618..635,636..641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    656..677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(794..811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gactatacaa atagcattcg tagaattcac aattcgatta tatttatact ggactatttt
       61 tacatctgtt cggttatttt cacatttatt tttctatata tatcttataa acgttttaaa
      121 acccatgtaa tttttgttaa gctgtaatat aaaagacgtc ctaacaaact tcttttatta
      181 ctgaatttcc tttaattata ataaataaca agttttaaaa taaattcagg caattaaggc
      241 gctcctgagg tactaaaatt aatgtaaaca tttaaaatta acttggatgg tcttaagtac
      301 tgtactcgtg attttgttat actttattat tagaaaagtc gtctattaac tttttgttcc
      361 ttaatttact tgattaaatt gtcgcttaat ttatcaaatc aggttttgcg cgttatttta
      421 gagaaaaact tattagaaaa atgaataagc aaagtttagg ctaacatgtt tttttattat
      481 tttaaatagt tcaagtcaat gacgtataaa ccagcttttg ttccctttag tgagggttaa
      541 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      841 tatcag
//
