LOCUS       sequence-426649-        1272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ficaretta ED, Singha Roy SJ, Voss L, Chatterjee A
  TITLE     Native Aminoacyl-tRNA Synthetase/tRNA Pair Drives Highly Efficient 
            Noncanonical Amino Acid Incorporation in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2024 Jun 24. doi: 10.1021/acschembio.4c00221.
  PUBMED    38913984
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2024 Jun 24. doi: 10.1021/acschembio.4c00221."
COMMENT     Sequence Label: pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg
       61 gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcgt gtgcggcttc
      121 gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc
      181 ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc
      241 gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg
      301 gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct
      361 gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc
      421 gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt
      481 ctcaaccgtg aaggtcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt
      541 tatagtatgg cctgtgcgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac
      601 cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg
      661 tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa
      721 ggcggcgcag tctggttaga tccgaagaaa accagcccgt acaaattcta ccagttctgg
      781 atcaacactg cgcgtgccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt
      841 gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag
      901 tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca
      961 aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc
     1021 gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg
     1081 caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc
     1141 tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa
     1201 gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg
     1261 atttgctgga aa
//
