LOCUS       sequence-42666-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     pHyVec Cloning Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHyVec11
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(686..706)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(686..704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(725..741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(738..760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    749..765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(773..779,780..797,798..803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 attatttagt tgaatttaaa tcaatttata tggctaaaaa accagttcaa ttaccaggat
       61 attattatgt agatgctaag ttagatatta catcacataa tgaagattat acaattgttg
      121 aacaatatga aagaacagaa ggtagacatc atttattttt atagcattcg tagattccaa
      181 gggcgaattc acaattcgat tatatttata ctggactatt tttacatctg ttcggttatt
      241 ttcacattta tttttctata tatatcttat aaacgtttta aaacccatgt aatttttgtt
      301 aagctgtaat ataaaagacg tcctaacaaa cttcttttat tactgaattt cctttaatta
      361 taataaataa caagttttaa aataaattca ggcaattaag gcgctcctga ggtactaaaa
      421 ttaatgtaaa catttaaaat taacttggat ggtcttaagt actgtactcg tgattttgtt
      481 atactttatt attagaaaag tcgtctatta actttttgtt ccttaattta cttgattaaa
      541 ttgtcgctta atttatcaaa tcaggttttg cgcgttattt tagagaaaaa cttattagaa
      601 aaatgaataa gcaaagttta ggctaacatg tttttttatt attttaaata gttcaagtca
      661 atgacgtata aaccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      781 gagccggaag cataaagtgt aaagcctggg
//
