LOCUS       sequence-426684-       13528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV-1 reporter vector that is env-deficient and expresses eGFP in 
            place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but 
            does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13528)
  AUTHORS   Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, 
            Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI,
            Johnson JS
  TITLE     Cell-free assays reveal that the HIV-1 capsid protects reverse 
            transcripts from cGAS immune sensing.
  JOURNAL   PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 
            10.1371/journal.ppat.1012206.
  PUBMED    39874383
REFERENCE   2  (bases 1 to 13528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2025-01-28"; volume: "21(1):e1012206. doi"; pages: 
            " 10.1371/journal.ppat.1012206"
COMMENT     Sequence Label: HIV-1-GFP Delta-Env CA Q63/67A
FEATURES             Location/Qualifiers
     source          1..13528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(626..643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(797..1385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(877..896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1556..2347,2348..2416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2179..2198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2417..2521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2589..2607
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2645..2667)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2767..2786
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             4479..4736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             4892..6394
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHAAAMAMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             6187..9198
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8883..9000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9843..10076
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10261..10305
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10599..10625
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      10955..10964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10961..10963,10964..10966,10967..11680)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVATLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11006..11027)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11267..11286)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11614..11635
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             11881..12514
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 atttttgaga cagggtctca ctctgtcacc taggctggag tgcagtggtg caatcacagc
       61 tcactgcagc ctcaacgtcg taagctcaag cgatcctccc acctcagcct gcctggtagc
      121 tgagactaca agcgacgccc cagttaattt ttgtattttt ggtagaggca gcgttttgcc
      181 gtgtggccct ggctggtctc gaactcctgg gctcaagtga tccagcctca gcctcccaaa
      241 gtgctgggac aaccggggcc agtcactgca cctggcccta aaccataatt tctaatcttt
      301 tggctaattt gttagtccta caaaggcagt ctagtcccca ggcaaaaagg gggtttgttt
      361 cgggaaaggg ctgttactgt ctttgtttca aactataaac taagttcctc ctaaacttag
      421 ttcggcctac acccaggaat gaacaaggag agcttggagg ttagaagcac gatggaattg
      481 gttaggtcag atctctttca ctgtctgagt tataattttg caatggtggt tcaaagactg
      541 cccgcttctg acaccagtcg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2521 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2581 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     2641 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2701 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2761 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     2821 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     2881 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccaggggagg cagagattgc
     2941 agtaagctga gatcgcagca ctgcactcca gcctgggcga cagagtaaga ctctgtctca
     3001 aaaataaaat aaataaatca atcagatatt ccaatctttt cctttattta tttatttatt
     3061 ttctattttg gaaacacagt ccttccttat tccagaatta cacatatatt ctatttttct
     3121 ttatatgctc cagttttttt tagaccttca cctgaaatgt gtgtatacaa aatctaggcc
     3181 agtccagcag agcctaaagg taaaaaataa aataataaaa aataaataaa atctagctca
     3241 ctccttcaca tcaaaatgga gatacagctg ttagcattaa ataccaaata acccatcttg
     3301 tcctcaataa ttttaagcgc ctctctccac cacatctaac tcctgtcaaa ggcatgtgcc
     3361 ccttccgggc gctctgctgt gctgccaacc aactggcatg tggactctgc agggtcccta
     3421 actgccaagc cccacagtgt gccctgaggc tgccccttcc ttctagcggc tgcccccact
     3481 cggctttgct ttccctagtt tcagttactt gcgttcagcc aaggtctgaa actaggtgcg
     3541 cacagagcgg taagactgcg agagaaagag accagcttta cagggggttt atcacagtgc
     3601 accctgacag tcgtcagcct cacagggggt ttatcacatt gcaccctgac agtcgtcagc
     3661 ctcacagggg gtttatcaca gtgcaccctt acaatcattc catttgattc acaatttttt
     3721 tagtctctac tgtgcctaac ttgtaagtta aatttgatca gaggtgtgtt cccagagggg
     3781 aaaacagtat atacagggtt cagtactatc gcatttcagg cctccacctg ggtcttggaa
     3841 tgtgtccccc gaggggtgat gactacctca gttggatctc cacaggtcac agtgacacaa
     3901 gataaccaag acacctccca aggctaccac aatgggccgc cctccacgtg cacatggccg
     3961 gaggaactgc catgtcggag gtgcaagcac acctgcgcat cagagtcctt ggtgtggagg
     4021 gagggaccag cgcagcttcc agccatccac ctgatgaaca gaacctaggg aaagccccag
     4081 ttctacttac accaggaaag gctggaaggg ctaatttggt cccaaaaaag acaagagatc
     4141 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     4201 gggccaggga tcagatatcc actgaccttt ggatggtgct tcaagttagt accagttgaa
     4261 ccagagcaag tagaagaggc caatgaagga gagaacaaca gcttgttaca ccctatgagc
     4321 cagcatggga tggaggaccc ggagggagaa gtattagtgt ggaagtttga cagcctccta
     4381 gcatttcgtc acatggcccg agagctgcat ccggagtact acaaagactg ctgacatcga
     4441 gctttctaca agggactttc cgctggggac tttccaggga ggtgtggcct gggcgggact
     4501 ggggagtggc gagccctcag atgctacata taagcagctg ctttttgcct gtactgggtc
     4561 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4621 taagcctcaa taaagcttgc cttgagtgct caaagtagtg tgtgcccgtc tgttgtgtga
     4681 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4741 cgcccgaaca gggacttgaa agcgaaagta aagccagagg agatctctcg acgcaggact
     4801 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4861 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcgg tattaagcgg
     4921 gggagaatta gataaatggg aaaaaattcg gttaaggcca gggggaaaga aacaatataa
     4981 actaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     5041 tttagagaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     5101 aggatcagaa gaacttagat cattatataa tacaatagca gtcctctatt gtgtgcatca
     5161 aaggatagat gtaaaagaca ccaaggaagc cttagataag atagaggaag agcaaaacaa
     5221 aagtaagaaa aaggcacagc aagcagcagc tgacacagga aacaacagcc aggtcagcca
     5281 aaattaccct atagtgcaga acctccaggg gcaaatggta catcaggcca tatcacctag
     5341 aactttaaat gcatgggtaa aagtagtaga agagaaggct ttcagcccag aagtaatacc
     5401 catgttttca gcattatcag aaggagccac cccacaagat ttaaatacca tgctaaacac
     5461 agtgggggga catgccgcag ccatggccat gttaaaagag accatcaatg aggaagctgc
     5521 agaatgggat agattgcatc cagtgcatgc agggcctatt gcaccaggcc agatgagaga
     5581 accaagggga agtgacatag caggaactac tagtaccctt caggaacaaa taggatggat
     5641 gacacataat ccacctatcc cagtaggaga aatctataaa agatggataa tcctgggatt
     5701 aaataaaata gtaagaatgt atagccctac cagcattctg gacataagac aaggaccaaa
     5761 ggaacccttt agagactatg tagaccgatt ctataaaact ctaagagccg agcaagcttc
     5821 acaagaggta aaaaattgga tgacagaaac cttgttggtc caaaatgcga acccagattg
     5881 taagactatt ttaaaagcat tgggaccagg agcgacacta gaagaaatga tgacagcatg
     5941 tcagggagtg gggggacccg gccataaagc aagagttttg gctgaagcaa tgagccaagt
     6001 aacaaatcca gctaccataa tgatacagaa aggcaatttt aggaaccaaa gaaagactgt
     6061 taagtgtttc aattgtggca aagaagggca catagccaaa aattgcaggg cccctaggaa
     6121 aaagggctgt tggaaatgtg gaaaggaagg acaccaaatg aaagattgta ctgagagaca
     6181 ggctaatttt ttagggaaga tctggccttc ccacaaggga aggccaggga attttcttca
     6241 gagcagacca gagccaacag ccccaccaga agagagcttc aggtttgggg aagagacaac
     6301 aactccctct cagaagcagg agccgataga caaggaactg tatcctttag cttccctcag
     6361 atcactcttt ggcagcgacc cctcgtcaca ataaagatag gggggcaatt aaaggaagct
     6421 ctattagata caggagcaga tgatacagta ttagaagaaa tgaatttgcc aggaagatgg
     6481 aaaccaaaaa tgataggggg aattggaggt tttatcaaag taagacagta tgatcagata
     6541 ctcatagaaa tctgcggaca taaagctata ggtacagtat tagtaggacc tacacctgtc
     6601 aacataattg gaagaaatct gttgactcag attggctgca ctttaaattt tcccattagt
     6661 cctattgaga ctgtaccagt aaaattaaag ccaggaatgg atggcccaaa agttaaacaa
     6721 tggccattga cagaagaaaa aataaaagca ttagtagaaa tttgtacaga aatggaaaag
     6781 gaaggaaaaa tttcaaaaat tgggcctgaa aatccataca atactccagt atttgccata
     6841 aagaaaaaag acagtactaa atggagaaaa ttagtagatt tcagagaact taataagaga
     6901 actcaagatt tctgggaagt tcaattagga ataccacatc ctgcagggtt aaaacagaaa
     6961 aaatcagtaa cagtactgga tgtgggcgat gcatattttt cagttccctt agataaagac
     7021 ttcaggaagt atactgcatt taccatacct agtataaaca atgagacacc agggattaga
     7081 tatcagtaca atgtgcttcc acagggatgg aaaggatcac cagcaatatt ccagtgtagc
     7141 atgacaaaaa tcttagagcc ttttagaaaa caaaatccag acatagtcat ctatcaatac
     7201 atggatgatt tgtatgtagg atctgactta gaaatagggc agcatagaac aaaaatagag
     7261 gaactgagac aacatctgtt gaggtgggga tttaccacac cagacaaaaa acatcagaaa
     7321 gaacctccat tcctttggat gggttatgaa ctccatcctg ataaatggac agtacagcct
     7381 atagtgctgc cagaaaagga cagctggact gtcaatgaca tacagaaatt agtgggaaaa
     7441 ttgaattggg caagtcagat ttatgcaggg attaaagtaa ggcaattatg taaacttctt
     7501 aggggaacca aagcactaac agaagtagta ccactaacag aagaagcaga gctagaactg
     7561 gcagaaaaca gggagattct aaaagaaccg gtacatggag tgtattatga cccatcaaaa
     7621 gacttaatag cagaaataca gaagcagggg caaggccaat ggacatatca aatttatcaa
     7681 gagccattta aaaatctgaa aacaggaaag tatgcaagaa tgaagggtgc ccacactaat
     7741 gatgtgaaac aattaacaga ggcagtacaa aaaatagcca cagaaagcat agtaatatgg
     7801 ggaaagactc ctaaatttaa attacccata caaaaggaaa catgggaagc atggtggaca
     7861 gagtattggc aagccacctg gattcctgag tgggagtttg tcaatacccc tcccttagtg
     7921 aagttatggt accagttaga gaaagaaccc ataataggag cagaaacttt ctatgtagat
     7981 ggggcagcca atagggaaac taaattagga aaagcaggat atgtaactga cagaggaaga
     8041 caaaaagttg tccccctaac ggacacaaca aatcagaaga ctgagttaca agcaattcat
     8101 ctagctttgc aggattcggg attagaagta aacatagtga cagactcaca atatgcattg
     8161 ggaatcattc aagcacaacc agataagagt gaatcagagt tagtcagtca aataatagag
     8221 cagttaataa aaaaggaaaa agtctacctg gcatgggtac cagcacacaa aggaattgga
     8281 ggaaatgaac aagtagataa attggtcagt gctggaatca ggaaagtact atttttagat
     8341 ggaatagata aggcccaaga agaacatgag aaatatcaca gtaattggag agcaatggct
     8401 agtgatttta acctaccacc tgtagtagca aaagaaatag tagccagctg tgataaatgt
     8461 cagctaaaag gggaagccat gcatggacaa gtagactgta gcccaggaat atggcagcta
     8521 gattgtacac atttagaagg aaaagttatc ttggtagcag ttcatgtagc cagtggatat
     8581 atagaagcag aagtaattcc agcagagaca gggcaagaaa cagcatactt cctcttaaaa
     8641 ttagcaggaa gatggccagt aaaaacagta catacagaca atggcagcaa tttcaccagt
     8701 actacagtta aggccgcctg ttggtgggcg gggatcaagc aggaatttgg cattccctac
     8761 aatccccaaa gtcaaggagt aatagaatct atgaataaag aattaaagaa aattatagga
     8821 caggtaagag atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac
     8881 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     8941 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9001 cgggtttatt acagggacag cagagatcca gtttggaaag gaccagcaaa gctcctctgg
     9061 aaaggtgaag gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga
     9121 aaagcaaaga tcatcaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt
     9181 agacaggatg aggattaaca catggaaaag attagtaaaa caccatatgg gattggaagc
     9241 cataataaga attctgcaac aactgctgtt tatccatttc agaattgggt gtcgacatag
     9301 cagaataggc gttactcgac agaggagagc aagaaatgga gccagtagat cctagactag
     9361 agccctggaa gcatccagga agtcagccta aaactgcttg taccaattgc tattgtaaaa
     9421 agtgttgctt tcattgccaa gtttgtttca tgacaaaagc cttaggcatc tcctatggca
     9481 ggaagaagcg gagacagcga cgaagagctc atcagaacag tcagactcat caagcttctc
     9541 tatcaaagca gtaagtagta catgggcgcg cccatgtggc aggaagtagg aaaagcaatg
     9601 tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg gctgctatta
     9661 acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg aggaggcgat
     9721 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9781 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9841 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9901 atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca gcagaacaat
     9961 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaaa
    10021 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10081 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10141 agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg ggacagagaa
    10201 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10261 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    10321 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    10381 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    10441 ttatcgtttc agacccacct cccaatcccg aggggacccg acaggcccga aggaatagaa
    10501 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atccttagca
    10561 cttatctggg acgatctgcg gagcctgtgc ctcttcagct accaccgctt gagagactta
    10621 ctcttgattg taacgaggat tgtggaactt ctgggacgca gggggtggga agccctcaaa
    10681 tattggtgga atctcctaca atattggagt caggagctaa agaatagtgc tgttagcttg
    10741 ctcaatgcca cagccatagc agtagctgag gggacagata gggttataga agtagtacaa
    10801 ggagcttgta gagctattcg ccacatacct agaagaataa gacagggctt ggaaaggatt
    10861 ttgctataag atgggtggcg cggccgcact cagatcttga gctcaggctt ggattctgca
    10921 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
    10981 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
    11041 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
    11101 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtggcca ccctgaccta
    11161 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
    11221 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
    11281 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
    11341 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
    11401 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
    11461 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
    11521 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
    11581 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
    11641 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggtcgcg actcgagacc
    11701 tagaaaaaca tggagcaatc acaagtagca atacagcagc taacaatgct gcttgtgcct
    11761 ggctagaagc acaagaggag gaagaggtgg gttttccagt cacacctcag gtacctttaa
    11821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11881 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
    11941 cacaaggcta cttccctgat tggcagaact acacaccagg gccaggggtc agatatccac
    12001 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
    12061 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgaccctg
    12121 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
    12181 agctgcatcc ggagtacttc aagaactgct gacatcgagc ttgctacaag ggactttccg
    12241 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
    12301 gctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
    12361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    12421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    12481 agaccctttt agtcagtgtg gaaaatctct agcacccagg aggtagaggt tgcagtgagc
    12541 caagatcgcg ccactgcatt ccagcctggg caagaaaaca agactgtcta aaataataat
    12601 aataagttaa gggtattaaa tatatttata catggaggtc ataaaaatat atatatttgg
    12661 gctgggcgca gtggctcaca cctgcgcccg gccctttggg aggccgaggc aggtggatca
    12721 cctgagtttg ggagttccag accagcctga ccaacatgga gaaacccctt ctctgtgtat
    12781 ttttagtaga ttttatttta tgtgtatttt attcacaggt atttctggaa aactgaaact
    12841 gtttttcctc tactctgata ccacaagaat catcagcaca gaggaagact tctgtgatca
    12901 aatgtggtgg gagagggagg ttttcaccag cacatgagca gtcagttctg ccgcagactc
    12961 ggcgggtgtc cttcggttca gttccaacac cgcctgcctg gagagaggtc agaccacagg
    13021 gtgagggctc agtccccaag acataaacac ccaagacata aacacccaac aggtccaccc
    13081 cgcctgctgc ccaggcagag ccgattcacc aagacgggaa ttaggataga gaaagagtaa
    13141 gtcacacaga gccggctgtg cgggagaacg gagttctatt atgactcaaa tcagtctccc
    13201 caagcattcg gggatcagag tttttaagga taacttagtg tgtagggggc cagtgagttg
    13261 gagatgaaag cgtagggagt cgaaggtgtc cttttgcgcc gagtcagttc ctgggtgggg
    13321 gccacaagat cggatgagcc agtttatcaa tccgggggtg ccagctgatc catggagtgc
    13381 agggtctgca aaatatctca agcactgatt gatcttaggt tttacaatag tgatgttacc
    13441 ccaggaacaa tttggggaag gtcagaatct tgtagcctgt agctgcatga ctcctaaacc
    13501 ataatttctt ttttgttttt tttttttt
//
