LOCUS       sequence-426695-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter: 
            p35S-UbArg-PhiC31NLS-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P27
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    256..278
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    286..462
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(694..1719)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1739..2084)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2287..2309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2301..2318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2302..2318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2325..2344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2325..2343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3341..3686
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3777..4004
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             4074..5885
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="DTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGG
                     RFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPI
                     VSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELG
                     GYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREI
                     KTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILR
                     DPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAW
                     LDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQ
                     HEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERAN
                     LVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEA
                     PKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIE
                     KRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             5886..5906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6461..6485
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6576..7164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6656..6675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8673..9488)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8673..9488)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9396..9415
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9480..9499
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9779..10085,1..129)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tatgttcaat tagcttttca attatgaata tatatatcaa ttattggtcg cccttggctt
       61 gtggacaatg cgctacgcgc accggctccg cccgtggaca accgcaagcg gttgcccacc
      121 gtcgagcgcc tttgcccaca acccggcggc cggccgcaac agatcgtttt ataaattttt
      181 ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc gggcttctgg atttccgatc
      241 cccggaatta gatcttggca ggatatattg tggtgtaacg ttatcgatct ggattttagt
      301 actggatttt ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa
      361 tacatactaa gggtttctta tatgctcaac acatgagcga aaccctataa gaaccctaat
      421 ttcccttatc gggaaactac tcacacatta tttatggaga aaatagagag agatagattt
      481 gtagagagag actggtgatt tcagcgtacc gaattaattc ctgaatcact gcgaccggcc
      541 ctcccgcgac ccagccgagc gagcttagcg aactgtggac gagaactgtg ccaccaagcg
      601 taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc tgaggcgttc
      661 tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg gacgagtgct
      721 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
      781 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
      841 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
      901 ttggtcaaga ccaatgcgga gcatatacgc ccggagcctt ggcgatcctg caagctccgg
      961 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     1021 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     1081 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     1141 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     1201 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     1261 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     1321 gaacccgctc gtctggctaa gatcggccgc agcgatagca tccatagcct ccgcgaccgg
     1381 ctgaagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     1441 gcgggagatg caataggtca ggctctcgct gaactcccca atgtcaagca cttccggaat
     1501 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     1561 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     1621 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     1681 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata ggggggatca gcttgggctg
     1741 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     1801 attgtgcgtc atcccttacg tcagtggaga tgtcacatca atccacttgc tttgaagacg
     1861 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     1921 cactgtcggc agaggcatct tgaatgatag cctttccttt atcgcaatga tggcatttgt
     1981 aggagccacc ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga
     2041 atccgaggag gtttcccgaa attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     2101 ctgagactgt atctttgaca tttttggagt aggggtacga taacattaac gcttacaatt
     2161 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2281 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     2341 agggcttgga ggaattccaa tcccacaaaa atctgagctt aacagcacag ttgctcctct
     2401 cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc
     2461 acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg
     2521 agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac
     2581 aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc
     2641 caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct
     2701 aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat
     2761 tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga
     2821 cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc
     2881 tgacccacag atggttagag aggcctacgc agcaagtctc atcaagacga tctacccgag
     2941 taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
     3001 caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat
     3061 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
     3121 ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg
     3181 aggatctaac agaactcgcc gtcaagactg gcgaacagtt catacagagt cttttacgac
     3241 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca
     3301 aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt caacaaagga
     3361 taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     3421 cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
     3481 ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     3541 tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt gacatctcca
     3601 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     3661 gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt tacaacaatt
     3721 accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc gatacaatgc
     3781 aaatcttcgt taaaactctg acgggcaaaa cgatcacgtt agaagtagag ccatccgata
     3841 cgatagaaaa cgtgaaggct aaaattcagg acaaagaggg aataccgcct gaccaacaga
     3901 ggctcatttt tgcaggcaag cagcttgaag atggtcgaac actgagtgat tacaacatcc
     3961 aaaaggagag cactctgcat ctagtcttac gtctgcgagg gggccgtcat ggtagcggtg
     4021 cgtggttgct gcccgtgagt ctcgtgaaga ggaagacgac tcttgctgga gccgacacgt
     4081 atgccggcgc ttatgataga caatcaagag aaagagagaa ctcttctgcc gcctctccag
     4141 ctacacagag atctgccaac gaggataaag ctgctgattt acaaagagag gtagagagag
     4201 atgggggtag gtttagattt gtgggtcatt tctctgaggc tcctggtaca tcagcctttg
     4261 gtactgctga acggcctgaa tttgaaagaa tattaaatga gtgtcgggcc ggtcgactaa
     4321 atatgataat agtttatgat gtatctaggt tcagtagact caaagttatg gatgcaattc
     4381 cgatcgtgag cgagctactt gcactcggtg ttactatcgt aagtacacaa gaaggtgtat
     4441 tcaggcaggg taatgtgatg gaccttatac accttattat gcgactcgac gctagccata
     4501 aagagtcatc actgaagagt gctaaaatct tggacaccaa gaacctgcaa agggagcttg
     4561 gaggctatgt aggaggaaaa gctccgtatg gtttcgaact cgtgagtgag acaaaagaga
     4621 ttaccagaaa tggtaggatg gtgaacgtgg ttattaataa gctagctcat agcacaaccc
     4681 ctctcacagg gccttttgag ttcgaacctg acgttattag atggtggtgg agagagatca
     4741 aaactcacaa acatcttcca tttaagccag gtagccaggc tgctatccac cctggatcta
     4801 tcactgggct ttgtaaaagg atggatgctg acgcagtgcc aactcgaggg gaaacaatcg
     4861 gtaagaagac tgcatcttcc gcttgggatc ccgctacagt tatgagaatc cttcgggatc
     4921 cgcgtattgc tggattcgcc gcagaagtca tttataagaa aaaacctgat ggtactccta
     4981 ctacaaaaat cgaaggttat aggatccaaa gggatcctat tactttgcgt ccagttgaat
     5041 tagactgtgg accgatcatc gaacctgccg agtggtacga gttgcaggcc tggcttgatg
     5101 gtagagggcg tggaaagggt ttatctcggg gacaggcaat tttgtcagca atggataagc
     5161 tttattgtga gtgtggggct gttatgacat caaaacgtgg agaagagtca atcaaggatt
     5221 cttacaggtg cagacgtcgt aaagtggtgg acccttctgc tccaggacag catgagggta
     5281 catgcaacgt ttctatggct gctcttgata aatttgtggc cgaacgtatt ttcaacaaga
     5341 ttagacacgc tgaaggggat gaggaaactt tagctcttct ttgggaggct gcccggcgtt
     5401 ttggtaagct aacagaggca ccagaaaaaa gtggcgaaag agctaatttg gtagccgaaa
     5461 gagcagacgc cttgaatgca ttggaagaac tttacgaaga tagggctgcc ggagcatatg
     5521 atggaccagt tggtcggaag catttcagga agcaacaagc tgcactaact ttgagacagc
     5581 aaggcgcaga ggagcgactc gcagagcttg aggccgccga ggccccaaag cttccactcg
     5641 atcaatggtt tcctgaggat gccgacgctg atcctacagg tccaaagagc tggtggggaa
     5701 gagctagcgt agacgataag agagtgtttg tgggtctatt tgttgacaag attgtagtta
     5761 ctaagtccac tacgggtcga ggacagggca ctcccatcga aaagagagcc agtattacct
     5821 gggcaaagcc tccaaccgat gatgatgaag acgatgcaca agatggtacc gaggatgtgg
     5881 cagcaccaaa aaaaaagagg aaggtgtaag cttagagact cttatcaaga atcccatctc
     5941 ttgcttgctt ttttttgttg tcttcccttt gatagggttt gtttttcttg tttcagtgac
     6001 tttctatgtt aaaagataat gtcagtaaaa ggatttggtt ttctattatt ctgaatcgat
     6061 tacggaagat tcttgcttaa ttccaatcta tacaagtatc gtgaaataat gaccgtttat
     6121 gtgattagga gacgtgtttc attaataaaa tataagatca atacattgtt agtagtgata
     6181 aactatgtac aaattgtatt gattgtaaaa gaaacacaat aggttccttt tttctacaat
     6241 atattgtgac agactctctg ttttaacgaa tgaattaaat ttgtcaacga aaaaattaaa
     6301 gaatgatgtg atcactttgc accttggttc atcttcagag gcttctgcat gttccatgca
     6361 ttgaggctct ctacggtcac aggttgtgag ccgttgttaa agacaaacaa atgcgctacc
     6421 acagaatcag gggataacgc aggaaagaac atgaaggcct tgacaggata tattggcggg
     6481 taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag
     6541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaagaagagt tggtagctct
     7081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtac tgagagatcc
     7321 cctcataatt tccccaaagc gtaaccatgt gtgaataaat tttgagctag tagggttgca
     7381 gccacgagta agtcttccct tgttattgtg tagccagaat gccgcaaaac ttccatgcct
     7441 aagcgaactg ttgagagtac gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc
     7501 caaccttgtt tctgagcatg aacgcccgca agccaacatg ttagttgaag catcagggcg
     7561 attagcagca tgatatcaaa acgctctgag ctgctcgttc ggctatggcg taggcctagt
     7621 ccgtaggcag gacttttcaa gtctcggaag gtttcttcaa tctgcattcg cttcgaatag
     7681 atattaacaa gttgtttggg tgttcgaatt tcaacaggta agttagttgc tagaacccat
     7741 ggctcctttg ccgacgctga gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc
     7801 gagcgctgat tttttcggcc tttagagcga gatttataca atagaatttg gcatgagatt
     7861 ggattgcttt tagtcagcct cttatagcct aaagtctttg agtgactaga tgacatatca
     7921 tgtaagttgc tgataggttt ccagttttcc gctcctaggt ctgcatattg tacttttcct
     7981 cttactcgac ttaaccagta ccaacccagc ttctcaacgg atttatacca tggcacttta
     8041 aagccagcat cactgacaat gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg
     8101 gctagaaatt ggtcatgagc tttctttgaa cattgctctg aaagcgggaa cgctttctca
     8161 taaagagtaa cagaacgacc gtgtagtgcg actgaagctc gcaataccat aagtcgtttt
     8221 tgctcacgaa tatcagacca gtcaacaagt acaatgggca tcgtattgcc cgaacagata
     8281 aagctagcat gccaacggta tacagcgagt cgctctttgt ggaggtgacg attacctaac
     8341 aatcggtcga ttcgtttgat gttatgtttt gttctcgctt tggttggcag gttacggcca
     8401 agttcggtaa gagtgagagt tttacagtca agtaatgcgt ggcaagccaa cgttaagctg
     8461 ttgagtcgtt ttaagtgtaa ttcggggcag aattggtaaa gagagtcgtg taaaatatcg
     8521 agttcgcaca tcttgttgtc tgattattga tttttcgcga aaccatttga tcatatgaca
     8581 agatgtgtat ccaccttaac ttaatgattt ttaccaaaat cattagggga ttcatcagtc
     8641 taaagtatat atgtgtaaca ttggtctagt gattagaaaa actcatcgag catcaaatga
     8701 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     8761 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     8821 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     8881 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     8941 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     9001 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     9061 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     9121 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     9181 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     9241 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     9301 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     9361 aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     9421 aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata
     9481 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     9541 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     9601 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     9661 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     9721 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcgatcccc
     9781 atccaacagc ccgccgtcga gcgggctttt ttatccccgg aagcctgtgg atagagggta
     9841 gttatccacg tgaaaccgct aatgccccgc aaagccttga ttcacggggc tttccggccc
     9901 gctccaaaaa ctatccacgt gaaatcgcta atcagggtac gtgaaatcgc taatcggagt
     9961 acgtgaaatc gctaataagg tcacgtgaaa tcgctaatca aaaaggcacg tgagaacgct
    10021 aatagccctt tcagatcaac agcttgcaaa cacccctcgc tccggcaagt agttacagca
    10081 agtag
//
