LOCUS       sequence-426696-       12992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9 without a targeting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, 
            Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI,
            Johnson JS
  TITLE     Cell-free assays reveal that the HIV-1 capsid protects reverse 
            transcripts from cGAS immune sensing.
  JOURNAL   PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 
            10.1371/journal.ppat.1012206.
  PUBMED    39874383
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2025-01-28"; volume: "21(1):e1012206. doi"; pages: 
            " 10.1371/journal.ppat.1012206"
COMMENT     Sequence Label: LCV2 control
FEATURES             Location/Qualifiers
     source          1..12992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..182
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             201..575
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    705..826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(742..761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     796..815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(875..891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(875..891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(888..910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    899..915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(923..929,930..947,948..953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    968..989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1106..1123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1277..1865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1357..1376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2036..2827,2828..2896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2659..2678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2897..3001)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3076..3095)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3267..3646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3648..3846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3801..3821
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3864..4044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4091..4216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4709..4942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5127..5171
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5469..5586
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5637..5885
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5637..5657
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5808..5827
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5886..5961
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6023..6234
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6253..6262
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6259..10362
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6259..10362
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10363..10410
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             10411..10434
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10444..10500
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10501..11097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     10994..11014
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11113..11701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11166..11186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11584..11595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11773..12006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(12032..12049)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12038..12262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12308..12736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12395..12414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12605..12626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12745..12765)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12750..12992,1..87)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12930..12992,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     join(12992,1..19)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
      121 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
      181 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
      241 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
      301 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
      361 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
      421 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
      481 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
      541 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
      601 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
      661 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
      721 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      781 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
      841 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1021 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1081 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1681 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3001 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     3061 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     3121 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     3181 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     3241 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     3301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3841 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3901 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3961 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4021 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     4081 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4141 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4201 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4261 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     4321 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     4381 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     4441 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     4501 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     4561 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     4621 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     4681 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     4741 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     4801 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     4861 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     4921 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     4981 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     5041 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     5101 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     5161 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     5221 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     5281 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     5341 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     5401 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     5461 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5521 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5581 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa ggtaccgagg
     5641 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     5701 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     5761 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     5821 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     5881 acaccgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     5941 aaagtggcac cgagtcggtg cttttttgaa ttcgctagct aggtcttgaa aggagtggga
     6001 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     6061 gggggagggg tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6121 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6181 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg
     6241 ttctagagcg ctgccaccat ggacaagaag tacagcatcg gcctggacat cggcaccaac
     6301 tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg
     6361 ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac
     6421 agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga
     6481 cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac
     6541 gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag
     6601 cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc
     6661 atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc
     6721 tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg
     6781 aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag
     6841 ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc
     6901 agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag
     6961 aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc
     7021 aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac
     7081 ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag
     7141 aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag
     7201 gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg
     7261 ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag
     7321 agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag
     7381 ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac
     7441 agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc
     7501 cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag
     7561 gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct
     7621 ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc
     7681 ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg
     7741 atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg
     7801 tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg
     7861 agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag
     7921 accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc
     7981 ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac
     8041 cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac
     8101 attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa
     8161 cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg
     8221 agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag
     8281 tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg
     8341 cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc
     8401 ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag
     8461 aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac
     8521 aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag
     8581 aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag
     8641 atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac
     8701 tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc
     8761 gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc catcgacaac
     8821 aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag
     8881 gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag
     8941 agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc
     9001 ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc
     9061 ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa
     9121 gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa
     9181 gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga
     9241 accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag
     9301 gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc
     9361 aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac
     9421 ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg
     9481 gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc
     9541 gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg
     9601 aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc
     9661 gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc
     9721 aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc
     9781 gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg
     9841 atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg
     9901 gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac
     9961 ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag
    10021 aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc
    10081 gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac
    10141 aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc
    10201 ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag
    10261 aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc
    10321 ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaagcgacc tgccgccaca
    10381 aagaaggctg gacaggctaa gaagaagaaa gattacaaag acgatgacga taagggatcc
    10441 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
    10501 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
    10561 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
    10621 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
    10681 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
    10741 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
    10801 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
    10861 gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc
    10921 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
    10981 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
    11041 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
    11101 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    11161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    11221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    11281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    11341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    11401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    11461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    11521 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    11581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    11641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    11701 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
    11761 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
    11821 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    11881 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    11941 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    12001 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
    12061 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
    12121 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
    12181 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
    12241 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
    12301 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    12361 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    12421 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    12481 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    12541 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    12601 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    12661 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    12721 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
    12781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
    12841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    12901 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    12961 attctccgcc ccatggctga ctaatttttt tt
//
