LOCUS       sequence-42670-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, 
            Heliot L, McNally JG
  TITLE     Concurrent fast and slow cycling of a transcriptional activator at 
            an endogenous promoter.
  JOURNAL   Science. 2008 Jan 25;319(5862):466-9.
  PUBMED    18218898
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2008-01-25"; volume: "319(5862)"; pages: "466-9"
COMMENT     Sequence Label: pTSK368
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4..20
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        60..410
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             786..806
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctcgaggtc gacggtatcg atatcttcgg atgcaagggt tcgaatccct tagctctcat
       61 tattttttgc tttttctctt gaggtcacat gatcgcaaaa tggcaaatgg cacgtgaagc
      121 tgtcgatatt ggggaactgt ggtggttggc aaatgactaa ttaagttagt caaggcgcca
      181 tcctcatgaa aactgtgtaa cataataacc gaagtgtcga aaaggtggca ccttgtccaa
      241 ttgaacacgc tcgatgaaaa aaataagata tatataaggt taagtaaagc gtctgttaga
      301 aaggaagttt ttcctttttc ttgctctctt gtcttttcat ctactatttc cttcgtgtaa
      361 tacagggtcg tcagatacat agatacaatt ctattacccc catccataca ggatcccgaa
      421 tggcttctaa ctttactcag ttcgttctcg tcgacaatgg cggaactggc gacgtgactg
      481 tcgccccaag caacttcgct aacgggatcg ctgaatggat cagctctaac tcgcgttcac
      541 aggcttacaa agtaacctgt agcgttcgtc agagctctgc gcagaatcgc aaatacacca
      601 tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt aaatatggaa ctaaccattc
      661 caattttcgc cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa
      721 aagatggaaa cccgattccc tcagcaatcg cagcaaactc cggcatctac gatgagctgt
      781 acaagccaaa aaagaagaga aaggtagcgg ccgccgctgc tgccgctgca gctgctgcag
      841 ctgcgatgtc taaaggtgaa gaattattca ctggtgttgt cc
//
