LOCUS       sequence-426700-        6801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for Pseudomonas aeruginosa with araC-ParaBAD 
            inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Meisner J, Goldberg JB
  TITLE     The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows 
            Tightly Controlled Gene Expression over a Wide Range in Pseudomonas 
            aeruginosa.
  JOURNAL   Appl Environ Microbiol. 2016 Oct 27;82(22):6715-6727. doi: 
            10.1128/AEM.02041-16. Print 2016 Nov 15.
  PUBMED    27613678
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02041-16"; journalName: "Appl Environ Microbiol"; date:
            "2016-10-27- 27"; volume: "82"; issue: "22"; pages: "6715-6727"
COMMENT     Sequence Label: pJM251 (miniCTX1-araC-ParaBAD)
FEATURES             Location/Qualifiers
     source          1..6801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            2021..2129
                     /note="incP origin of transfer"
     protein_bind    2336..2383
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2557..2576
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2557..2575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2600..2616
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2601..2617
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2672..2956)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(2730..2749)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2983..3861
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(3891..3911)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3891..3909)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    4448..4495
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(4714..4719,4720..4736,4737..4742)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             4790..5980
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(5142..5161)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatcc agcttggtag
      661 cctgaacgct tggtgtggat caggggaacc agatggcaga cggagtcgag gtacgcggaa
      721 aacggatcag gatctacttc cgctaccagg gcgagctgtg tagggagtcc ataccagggg
      781 acgcaacgcc cgagaatata gccaatgccg aacagctggc cggcatcatc aactacgaga
      841 tcaagcaagg agtcttcagc tacagccgac acttcccgga ctcaccgcgg gtgaaaagca
      901 acaccttggg ccattacatc gacctctggc tcgacatcaa acgcaaccag attgccgcca
      961 gcggcttccg cggctacacc agccgcgtgg aaacccacat ccgacctcgc tggggtgaca
     1021 gccaggccga cagcatcgac cacctggata ttcaagactg ggtgcagaac acgctcatgc
     1081 ccaagctgca caacaagacg gtgcgagaga tcgtcagcaa cctgcggcag atcttccggc
     1141 tgtatcgaac tcggaaccgg agcgcgcacg acccaacaga cggcatcgtg atcactcttc
     1201 cggatgcgga tgacccagac ccattcactc gtgaggaaat cgacctcatc ctcggtactg
     1261 aaaccgcgcg catcggtgag ctgaacctgg cggagttcat gatctggagc ggcccccgcg
     1321 tcagcgaggc catagctctg gcctgggaag atgtcgattt ggacacgggg accgtggttt
     1381 tccgccgcgc tcgtgtacgc agccagtaca aggtcaccaa gactcgccga tcgacgagga
     1441 aggtccagct actggccccg gcgctgcgcg cactccagca gcaggccaag ctaaccagac
     1501 gccttccgcc cgtacagatc gaggtgatag accgcgacaa ccggaccaga aagcctcaac
     1561 gggtacgctt cgtgttccac aacagtgcta gcggggcggc gtactccacg tcggatacgc
     1621 tgcgcaacgg ctggtggcat ggccacctca ggaatgcggg cgtccgctcg cgtggtccca
     1681 atcagtgccg ccacaccttc gccagccaga tgctcagcag cggcatcgcc actcccgaat
     1741 ggatcgcgga ccagatgggg cacacctcga cggcgatgat cttcaagcat tacgccaagt
     1801 ggatcagcaa ggacggcccc gacatcgtag gtctgctgaa tcaggcgctg aaactttcct
     1861 gaagcaacga aaaaggttaa ggtatacttt ccgctgcata accctgcttc ggggtcatta
     1921 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1981 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     2041 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     2101 acgggaatcc tgctctgcga ggctggccga taagctctag catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2281 aaccgcctct ccccgcgcgt tggccgattc attaatgcag cgaattgggg atcttgaagt
     2341 acctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaagct
     2401 gatgtgctta aaaacttact caatggaatt agctttatgc ttgtaaaccg ttttgtgaaa
     2461 aaatttttaa aataaaaaag gggacctcta gggtccccaa ttaattagta atataatcta
     2521 ttaaaggtca ttcaaaaggt catccaccgg cgcgcgtaat acgactcact atagggcgaa
     2581 ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttctcga ggaattcctg
     2641 cagagcacta gtctagccca aaaaaacggg tatggagaaa cagtagagag ttgcgataaa
     2701 aagcgtcagg taggatccgc taatcttatg gataaaaatg ctatggcata gcaaagtgtg
     2761 acgccgtgca aataatcaat gtggactttt ctgccgtgat tatagacact tttgttacgc
     2821 gtttttgtca tggctttggt cccgctttgt tacagaatgc ttttaataag cggggttacc
     2881 ggtttggtta gcgagaagag ccagtaaaag acgcagtgac ggcaatgtct gatgcaatat
     2941 ggacaattgg tttcttctct gaatggcggg agtatgaaaa gtatggctga agcgcaaaat
     3001 gatcccctgc tgccgggata ctcgtttaat gcccatctgg tggcgggttt aacgccgatt
     3061 gaggccaacg gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt
     3121 ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtttgc
     3181 cgaccgggtg atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg
     3241 gaggctcgcg aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa
     3301 tggcttaact ggccgtcaat atttgccaat acggggttct ttcgcccgga tgaagcgcac
     3361 cagccgcatt tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc
     3421 tattcggagc tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg
     3481 attaacgagt cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc
     3541 agcgatcacc tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcttg
     3601 tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg
     3661 cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga gcaccacccg gatgcctatc
     3721 gccaccgtcg gtcgcaatgt tggttttgac gatcaactct atttctcgcg ggtatttaaa
     3781 aaatgcaccg gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat
     3841 gtagccgtca agttgtcata atttggggat tcgagctcca gcttttgttc cctttagtga
     3901 gggttaattg cgcgggtgga tgaccttttg aatgaccttt aatagattat attactaatt
     3961 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa aacggtttac
     4021 aagcataaag ctaattcagc aaggacggcc ccgacatcgt aggtctgctg aatcaggcgc
     4081 tgaaactttc ctgaagcaac gaaaaagggg ccctgcgggg cccctttttt ctgcgcctcg
     4141 ttcccagttt gttcccaaat cgttcactcc tgagagtggg tcgaatggaa acatcaatga
     4201 aaacagcagg ttatatggcg gaggcggtga gattcgaact catatgaaac gccccaacct
     4261 cgctggaggc cgcattattc ctgggcccta ctgtccattt tcactcgcgt ttgttcccag
     4321 agtgttccca gcgtgtgcga gagagcctgg aagcctgccg tcaaacctgc cgtcccaggg
     4381 caacagccgg atttcatgtt gacccattga aatccattgc tgttgacaaa gggaatcagg
     4441 ggatcttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc
     4501 gcttttgaag ctaattcgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4561 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4621 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4681 aacgcaggaa agaacatgaa ttaattctca tgtttgacag cttatcatcg attagcttta
     4741 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
     4801 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
     4861 tatgccggta ctgccgggcc tcttgcggga tattgtccat tccgacagca tcgccagtca
     4921 ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
     4981 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
     5041 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg attctctacg ccggacgcat
     5101 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     5161 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     5221 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttacatg caccattcct
     5281 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     5341 gcataaggga gagcgccgac ccatgccctt gagagccttc aacccagtca gctccttccg
     5401 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     5461 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     5521 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     5581 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     5641 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     5701 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     5761 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac
     5821 cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag
     5881 cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc
     5941 gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc
     6001 gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat
     6061 gcgcaaacca acccttgact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6121 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6181 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6241 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6301 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6361 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6421 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6481 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6541 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6601 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6661 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6721 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6781 agaaaagatc aaaggatctt c
//
