LOCUS       sequence-426701-       11141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin 
            N-acetyl-transferase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Ehrlich M, Mozafari S, Glatza M, Starost L, Velychko S, Hallmann AL,
            Cui QL, Schambach A, Kim KP, Bachelin C, Marteyn A, Hargus G, 
            Johnson RM, Antel J, Sterneckert J, Zaehres H, Scholer HR, Baron-Van
            Evercooren A, Kuhlmann T
  TITLE     Rapid and efficient generation of oligodendrocytes from human 
            induced pluripotent stem cells using transcription factors.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2243-E2252. doi: 
            10.1073/pnas.1614412114. Epub 2017 Feb 28.
  PUBMED    28246330
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1614412114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2243-E2252"
COMMENT     Sequence Label: SF-SON IRES Puro
FEATURES             Location/Qualifiers
     source          1..11141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             390..446
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1428..1481
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(2330..2904,2905..2907,2908..2916)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2497..2514)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2724..2743
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2917..3516
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2917..2936)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3413..3433
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3529..4117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3582..3602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4000..4011)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4225..4272
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     LTR             4317..4497
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4569..4690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4606..4625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4660..4679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4730..4865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4885..4904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4886..4904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4914..4931)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4914..4930)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4923..4945)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5072..5527
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5159..5178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5369..5390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5553..5657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5658..5726,5727..6518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5876..5895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6689..7277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7178..7197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7431..7448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7565..7586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7601..7606,7607..7624,7625..7631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7639..7655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7644..7666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7663..7679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7663..7679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7698..7718
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7700..7718
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7746..7972
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7973..8153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8200..8325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8818..9051
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9236..9280
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9547..9664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9698..10104
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      10118..10127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggctctgcc cacggtctca ccacctggtg tggatgccaa agcccaggtg aagacagaga
       61 ccgcggggcc ccaggggccc ccacactaca ccgaccagcc atccacctca cagatcgcct
      121 acacctccct cagcctgccc cactatggct cagccttccc ctccatctcc cgcccccagt
      181 ttgactactc tgaccatcag ccctcaggac cctattatgg ccactcgggc caggcctctg
      241 gcctctactc ggccttctcc tatatggggc cctcgcagcg gcccctctac acggccatct
      301 ctgaccccag cccctcaggg ccccagtccc acagccccac acactgggag cagccagtat
      361 atacgacact gtcccggccc tcttccggag ccaccaactt ctccctgctg aagcaggccg
      421 gcgacgtgga ggagaacccc ggcccctcca tggactcgga cgccagcctg gtgtccagcc
      481 gcccgtcgtc gccagagccc gatgaccttt ttctgccggc ccggagtaag ggcagcagcg
      541 gcagcgcctt cactgggggc accgtgtcct cgtccacccc gagtgactgc ccgccggagc
      601 tgagcgccga gctgcgcggc gctatgggct ctgcgggcgc gcatcctggg gacaagctag
      661 gaggcagtgg cttcaagtca tcctcgtcca gcacctcgtc gtctacgtcg tcggcggctg
      721 cgtcgtccac caagaaggac aagaagcaaa tgacagagcc ggagctgcag cagctgcgtc
      781 tcaagatcaa cagccgcgag cgcaagcgca tgcacgacct caacatcgcc atggatggcc
      841 tccgcgaggt catgccgtac gcacacggcc cttcggtgcg caagctttcc aagatcacca
      901 cgctgctgct ggcgcgcaac tacatcctca tgctcaccaa ctcgctggag gagatgaagc
      961 gactggtgag cgagatctac gggggccacc acgctggctt ccacccgtcg gcctgcggcg
     1021 gcctggcgca ctccgcgccc ctgcccgccg ccaccgcgca cccggcagca gcagcgcacg
     1081 ccgcacatca ccccgcggtg caccacccca tcctgccgcc cgccgccgca gcggctgctg
     1141 ccgccgctgc agccgcggct gtgtccagcg cctctctgcc cggatccggg ctgccgtcgg
     1201 tcggctcaat ccgtccaccg cacggcctac tcaagtctcc gtctgctgcc gcggccgccc
     1261 cgctgggggg cgggggcggc ggcagtgggg cgagcggggg cttccagcac tggggcggca
     1321 tgccctgccc ctgcagcatg tgccaggtgc cgccgccgca ccaccacgtg tcggctatgg
     1381 gcgccggcag cctgccgcgc ctcacctccg acgccaaggg ctccggagag ggccggggct
     1441 ctctgctgac ctgtggcgac gtggaggaga accccggccc ctccatggac actaaccgcc
     1501 cgggcgcgtt cgtgctgagc agtgccccgc tggccgcgct gcacaacatg gccgagatga
     1561 agacgtcgct gttcccctac gcgctgcagg gtccggccgg cttcaaggcg cccgcgctgg
     1621 ggggcctggg cgcgcagctc ccgctcggga ccccgcacgg catcagcgac atcctgggcc
     1681 ggcccgtggg cgcggcgggc gggggcctcc tgggggggct gccccggctc aacgggctcg
     1741 cgtcgtccgc cggcgtttac ttcgggcccg cggccgctgt ggcgcgcggc taccccaagc
     1801 ccctggccga gctgccgggg cgcccgccca tcttctggcc cggcgtggtg cagggcgcgc
     1861 cctggaggga cccgcgtctg gctggcccgg ccccggccgg cggcgtcctg gacaaggacg
     1921 ggaagaagaa gcactcgcgc ccgaccttct cgggccagca gatcttcgcg ctggagaaaa
     1981 ccttcgagca gaccaagtac ctggcgggcc cggagcgcgc gcgtctcgcc tactcgctgg
     2041 gcatgaccga gagccaggtg aaggtctggt tccagaaccg ccggaccaag tggcgcaagc
     2101 ggcacgcggc ggagatggcg tcggccaaga agaagcagga ctcggacgcc gagaagctga
     2161 aggtgcgcgg ctcggacgcg gaggacgacg acgaatacaa ccggcccctg gaccccaact
     2221 cggacgacga gaagatcacg cggctgctca agaagcacaa accctcgaac ttggcgctgg
     2281 tcagcccgtg cggcggcggc gcgggggacg ccttgtgatg agtcgaccgc ccctctccct
     2341 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     2401 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     2461 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     2521 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     2581 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     2641 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     2701 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     2761 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     2821 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2881 tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg
     2941 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     3001 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     3061 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     3121 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     3181 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     3241 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     3301 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     3361 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     3421 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     3481 cgcacctggt gcatgacccg caagcccggt gcctgaatcg atgtcgacaa tcaacctctg
     3541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3661 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3721 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3781 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3841 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3901 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3961 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4021 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4081 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     4141 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4201 gactggaagg gctacgtagc tagcgaagtt cctattccga agttcctatt ctctagaaag
     4261 tataggaact tctacgtaac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4321 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4381 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4441 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4501 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4561 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4621 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4681 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4801 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4861 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4921 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4981 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5041 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5101 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5161 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5221 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5281 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5341 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5401 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5461 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5521 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5581 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5641 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5701 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5761 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5821 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5881 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5941 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6001 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6061 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6121 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6181 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6241 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6361 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7321 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7381 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7441 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7501 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7561 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7621 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7681 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7741 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7801 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7861 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7921 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8161 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8221 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8281 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8341 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8401 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8461 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8521 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8581 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8641 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8701 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8761 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8821 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8881 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8941 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9001 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9061 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9121 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9181 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9241 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9301 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9361 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9421 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     9481 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     9541 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9601 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9661 ttttatcgat cacgagacta gcctcgagct agctgcagta acgccatttt gcaaggcatg
     9721 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     9781 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     9841 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     9901 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     9961 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
    10021 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
    10081 cctccgacag actgagtcgg ccggtggatc caccggtgcc accatggcgg aggagcagga
    10141 cctatcggag gtggagctga gccccgtggg ctcggaggag ccccgctgcc tgtccccggg
    10201 gagcgcgccc tcgctagggc ccgacggcgg cggcggcgga tcgggcctgc gagccagccc
    10261 ggggccaggc gagctgggca aggtcaagaa ggagcagcag gacggcgagg cggacgatga
    10321 caagttcccc gtgtgcatcc gcgaggccgt cagccaggtg ctcagcggct acgactggac
    10381 gctggtgccc atgcccgtgc gcgtcaacgg cgccagcaaa agcaagccgc acgtcaagcg
    10441 gcccatgaac gccttcatgg tgtgggctca ggcagcgcgc aggaagctcg cggaccagta
    10501 cccgcacctg cacaacgctg agctcagcaa gacgctgggc aagctctgga ggctgctgaa
    10561 cgaaagtgac aagcgcccct tcatcgagga ggctgagcgg ctccgtatgc agcacaagaa
    10621 agaccacccg gactacaagt accagcccag gcggcggaag aacgggaagg ccgcccaggg
    10681 cgaggcggag tgccccggtg gggaggccga gcaaggtggg accgccgcca tccaggccca
    10741 ctacaagagc gcccacttgg accaccggca cccaggagag ggctccccca tgtcagatgg
    10801 gaaccccgag cacccctcag gccagagcca tggcccaccc acccctccaa ccaccccgaa
    10861 gacagagctg cagtcgggca aggcagaccc gaagcgggac gggcgctcca tgggggaggg
    10921 cgggaagcct cacatcgact tcggcaacgt ggacattggt gagatcagcc acgaggtaat
    10981 gtccaacatg gagacctttg atgtggctga gttggaccag tacctgccgc ccaatgggca
    11041 cccaggccat gtgagcagct actcagcagc cggctatggg ctgggcagtg ccctggccgt
    11101 ggccagtgga cactccgcct ggatctccaa gccaccaggc g
//
