LOCUS       sequence-426702-       11275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SOX10, OLIG2, NKX6.2 and tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Ehrlich M, Mozafari S, Glatza M, Starost L, Velychko S, Hallmann AL,
            Cui QL, Schambach A, Kim KP, Bachelin C, Marteyn A, Hargus G, 
            Johnson RM, Antel J, Sterneckert J, Zaehres H, Scholer HR, Baron-Van
            Evercooren A, Kuhlmann T
  TITLE     Rapid and efficient generation of oligodendrocytes from human 
            induced pluripotent stem cells using transcription factors.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2243-E2252. doi: 
            10.1073/pnas.1614412114. Epub 2017 Feb 28.
  PUBMED    28246330
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1614412114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2243-E2252"
COMMENT     Sequence Label: SF-SON IRES Tomato
FEATURES             Location/Qualifiers
     source          1..11275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      928..1516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1417..1436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1670..1687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1804..1825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1840..1845,1846..1863,1864..1870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1878..1894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1883..1905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1902..1918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1902..1918
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1937..1957
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1939..1957
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1985..2211
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2212..2392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2439..2564
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3057..3290
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3475..3519
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3786..3903
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3937..4343
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      4357..4366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5770..5826
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6808..6861
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(7736..8310,8311..8313,8314..8322)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7903..7920)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8130..8149
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8323..9024
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(8456..8474)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8724..8743
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8918..8941
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9043..9631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9096..9116)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9514..9525)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    9739..9786
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     LTR             9831..10011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10083..10204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10120..10139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10174..10193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10244..10379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10399..10418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10400..10418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10428..10445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10428..10444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10437..10459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10586..11041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10673..10692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10883..10904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11067..11171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11172..11240,11241..11275,1..757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1801 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1921 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     1981 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     2041 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     2101 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     2161 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     2221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2401 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2461 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2521 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2581 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2641 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2701 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     2761 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     2821 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     2881 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     2941 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     3001 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     3061 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     3121 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     3181 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     3241 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     3301 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3361 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3421 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3481 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3541 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3601 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     3661 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     3721 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     3781 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     3841 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     3901 tttatcgatc acgagactag cctcgagcta gctgcagtaa cgccattttg caaggcatgg
     3961 aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta
     4021 acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa
     4081 cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat
     4141 ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc
     4201 tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc
     4261 gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc
     4321 ctccgacaga ctgagtcggc cggtggatcc accggtgcca ccatggcgga ggagcaggac
     4381 ctatcggagg tggagctgag ccccgtgggc tcggaggagc cccgctgcct gtccccgggg
     4441 agcgcgccct cgctagggcc cgacggcggc ggcggcggat cgggcctgcg agccagcccg
     4501 gggccaggcg agctgggcaa ggtcaagaag gagcagcagg acggcgaggc ggacgatgac
     4561 aagttccccg tgtgcatccg cgaggccgtc agccaggtgc tcagcggcta cgactggacg
     4621 ctggtgccca tgcccgtgcg cgtcaacggc gccagcaaaa gcaagccgca cgtcaagcgg
     4681 cccatgaacg ccttcatggt gtgggctcag gcagcgcgca ggaagctcgc ggaccagtac
     4741 ccgcacctgc acaacgctga gctcagcaag acgctgggca agctctggag gctgctgaac
     4801 gaaagtgaca agcgcccctt catcgaggag gctgagcggc tccgtatgca gcacaagaaa
     4861 gaccacccgg actacaagta ccagcccagg cggcggaaga acgggaaggc cgcccagggc
     4921 gaggcggagt gccccggtgg ggaggccgag caaggtggga ccgccgccat ccaggcccac
     4981 tacaagagcg cccacttgga ccaccggcac ccaggagagg gctcccccat gtcagatggg
     5041 aaccccgagc acccctcagg ccagagccat ggcccaccca cccctccaac caccccgaag
     5101 acagagctgc agtcgggcaa ggcagacccg aagcgggacg ggcgctccat gggggagggc
     5161 gggaagcctc acatcgactt cggcaacgtg gacattggtg agatcagcca cgaggtaatg
     5221 tccaacatgg agacctttga tgtggctgag ttggaccagt acctgccgcc caatgggcac
     5281 ccaggccatg tgagcagcta ctcagcagcc ggctatgggc tgggcagtgc cctggccgtg
     5341 gccagtggac actccgcctg gatctccaag ccaccaggcg tggctctgcc cacggtctca
     5401 ccacctggtg tggatgccaa agcccaggtg aagacagaga ccgcggggcc ccaggggccc
     5461 ccacactaca ccgaccagcc atccacctca cagatcgcct acacctccct cagcctgccc
     5521 cactatggct cagccttccc ctccatctcc cgcccccagt ttgactactc tgaccatcag
     5581 ccctcaggac cctattatgg ccactcgggc caggcctctg gcctctactc ggccttctcc
     5641 tatatggggc cctcgcagcg gcccctctac acggccatct ctgaccccag cccctcaggg
     5701 ccccagtccc acagccccac acactgggag cagccagtat atacgacact gtcccggccc
     5761 tcttccggag ccaccaactt ctccctgctg aagcaggccg gcgacgtgga ggagaacccc
     5821 ggcccctcca tggactcgga cgccagcctg gtgtccagcc gcccgtcgtc gccagagccc
     5881 gatgaccttt ttctgccggc ccggagtaag ggcagcagcg gcagcgcctt cactgggggc
     5941 accgtgtcct cgtccacccc gagtgactgc ccgccggagc tgagcgccga gctgcgcggc
     6001 gctatgggct ctgcgggcgc gcatcctggg gacaagctag gaggcagtgg cttcaagtca
     6061 tcctcgtcca gcacctcgtc gtctacgtcg tcggcggctg cgtcgtccac caagaaggac
     6121 aagaagcaaa tgacagagcc ggagctgcag cagctgcgtc tcaagatcaa cagccgcgag
     6181 cgcaagcgca tgcacgacct caacatcgcc atggatggcc tccgcgaggt catgccgtac
     6241 gcacacggcc cttcggtgcg caagctttcc aagatcacca cgctgctgct ggcgcgcaac
     6301 tacatcctca tgctcaccaa ctcgctggag gagatgaagc gactggtgag cgagatctac
     6361 gggggccacc acgctggctt ccacccgtcg gcctgcggcg gcctggcgca ctccgcgccc
     6421 ctgcccgccg ccaccgcgca cccggcagca gcagcgcacg ccgcacatca ccccgcggtg
     6481 caccacccca tcctgccgcc cgccgccgca gcggctgctg ccgccgctgc agccgcggct
     6541 gtgtccagcg cctctctgcc cggatccggg ctgccgtcgg tcggctcaat ccgtccaccg
     6601 cacggcctac tcaagtctcc gtctgctgcc gcggccgccc cgctgggggg cgggggcggc
     6661 ggcagtgggg cgagcggggg cttccagcac tggggcggca tgccctgccc ctgcagcatg
     6721 tgccaggtgc cgccgccgca ccaccacgtg tcggctatgg gcgccggcag cctgccgcgc
     6781 ctcacctccg acgccaaggg ctccggagag ggccggggct ctctgctgac ctgtggcgac
     6841 gtggaggaga accccggccc ctccatggac actaaccgcc cgggcgcgtt cgtgctgagc
     6901 agtgccccgc tggccgcgct gcacaacatg gccgagatga agacgtcgct gttcccctac
     6961 gcgctgcagg gtccggccgg cttcaaggcg cccgcgctgg ggggcctggg cgcgcagctc
     7021 ccgctcggga ccccgcacgg catcagcgac atcctgggcc ggcccgtggg cgcggcgggc
     7081 gggggcctcc tgggggggct gccccggctc aacgggctcg cgtcgtccgc cggcgtttac
     7141 ttcgggcccg cggccgctgt ggcgcgcggc taccccaagc ccctggccga gctgccgggg
     7201 cgcccgccca tcttctggcc cggcgtggtg cagggcgcgc cctggaggga cccgcgtctg
     7261 gctggcccgg ccccggccgg cggcgtcctg gacaaggacg ggaagaagaa gcactcgcgc
     7321 ccgaccttct cgggccagca gatcttcgcg ctggagaaaa ccttcgagca gaccaagtac
     7381 ctggcgggcc cggagcgcgc gcgtctcgcc tactcgctgg gcatgaccga gagccaggtg
     7441 aaggtctggt tccagaaccg ccggaccaag tggcgcaagc ggcacgcggc ggagatggcg
     7501 tcggccaaga agaagcagga ctcggacgcc gagaagctga aggtgcgcgg ctcggacgcg
     7561 gaggacgacg acgaatacaa ccggcccctg gaccccaact cggacgacga gaagatcacg
     7621 cggctgctca agaagcacaa accctcgaac ttggcgctgg tcagcccgtg cggcggcggc
     7681 gcgggggacg ccttgtgatg agtcgacgaa ttcggtaccg cgggcccggg atccgcccct
     7741 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     7801 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     7861 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     7921 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     7981 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     8041 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     8101 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     8161 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     8221 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     8281 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
     8341 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     8401 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     8461 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     8521 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     8581 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     8641 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     8701 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     8761 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     8821 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     8881 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     8941 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     9001 tacggcatgg acgagctgta caagtaaagc ggccgcgtcg acaatcaacc tctggattac
     9061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9421 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     9481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9601 agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac
     9661 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9721 aagggctacg tagctagcga agttcctatt ccgaagttcc tattctctag aaagtatagg
     9781 aacttctacg taactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     9841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
    10021 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    10081 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10201 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    10261 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10321 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10381 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    10441 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    10501 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    10561 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10741 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10801 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10861 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10921 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    10981 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    11041 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    11101 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    11161 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    11221 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctga
//
