LOCUS       sequence-426703-       14788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS 
            Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA 
            Q67H/N74D)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, 
            Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI,
            Johnson JS
  TITLE     Cell-free assays reveal that the HIV-1 capsid protects reverse 
            transcripts from cGAS immune sensing.
  JOURNAL   PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 
            10.1371/journal.ppat.1012206.
  PUBMED    39874383
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2025-01-28"; volume: "21(1):e1012206. doi"; pages: 
            " 10.1371/journal.ppat.1012206"
COMMENT     Sequence Label: SG3-Delta-ENV CA Q67H/N74D
FEATURES             Location/Qualifiers
     source          1..14788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             438..449
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(2041..5052)
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSSTRRELQVWGGDNNSPSEAG
                     AGRQGNVSLSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYSGIKVKQLCKLLRGTKALTEVVTLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARTRGAHT
                     NDVKQLTEAVQKITTECIIIWGKTPKFRLPIQKETWETWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIEGAETFYVDGAANRETKLGKAGYVTNKGRQKVVTLTDTTNQKTELE
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGVRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVVESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDNKDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    2239..2356
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6331..6456
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6503..6759
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8902..8919)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8902..8918)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8911..8933)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9059..9514)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9196..9217)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9408..9427
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9541..9645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9646..9714,9715..10506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9864..9883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10677..11265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11166..11185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11419..11436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11553..11574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11589..11594,11595..11612,11613..11619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11627..11643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11632..11654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11651..11667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11651..11667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11686..11705
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11686..11704
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             11985..11993
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     LTR             12194..12450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(13682..13726)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13911..14144
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gttgggtctt gtgcaattaa tttctacagc ttctttcagc tgtactatta tggttttagc
       61 attgtccgag aaattggcag atctaattac tacctcttct tctgctaggc tgccatttaa
      121 cagcagttga gttgatacta ctggcttaat tccatgtgta cattgtactg tgctgacatt
      181 tttacatgat cctgttccat tgaacttctt atctttacac tttagaatcg caaaaccagc
      241 cggggcacaa taatgtatgg gaattggctc aaaggatatc tttggacagg cctgtgtagt
      301 gactgaggtg ttacaactta tcaacctata tttggtacta ttcttatcat tatctattgg
      361 tactacatca agtttataaa agagtgcata tgctttctgc ttcttatctc ctaagacatt
      421 gaaagagcag ttctttattt ctcctctttc catctttttc cagctaccac taggggtatt
      481 agtactagct agtggtatta gtagcattct tcaactcatc agtgcaattt agagtaacac
      541 agagtggggt taattttaca catggcttta ggctttgatc ccataaactg attatatcct
      601 cctgcatctg ttctaccatg ttatttttcc acatgttaaa atcttctgtc acattttcta
      661 atactatctc ttgtgggttg gggtctgtgg gtatacaggc atgtgtggcc caaacattat
      721 gtacctctgt atcatatgct ttagcatctg atgcacaaaa tagagtggtg gttgcttctt
      781 tccatacagg taccccataa tatactgtga cccacaaatt ccctgcagca ctatagatca
      841 tcaatatccc aaagagcatg gtgccccatc tccacaagtg ctgataattc ttcctgatct
      901 ccttcactct cattgccact gtcttctgct cttttactta ttcttttaat taacctgtct
      961 atttttcttt gtcttaatat tttcctatat tctattaata ctatggacca cacaactatt
     1021 gctattattc ctaccactac taatgctact attgctgcta tttgtatagg ttgcattaca
     1081 tgcactactt actgctttga tagagaagct tgatgagcct gactgtcttg aggagctctt
     1141 cgtcgctgtc tccgcttctt cctgccatag gagatgccta agccttttgt catgaaacaa
     1201 acttggcaat gtaagcaaca ctttttacaa tagcatttgg tacaagcagt tttaggctgg
     1261 cttcctggat gcttccaggg ctctagtcta ggatctactg gctccatttc ttgctcttct
     1321 tcgttgagta atgcctattc tgctatgtcg acacccaatt ctgaaaagag taaacagcag
     1381 ttgttgcaga gttcttacta gggcttccac tcctgcccaa gtatccccat aagtttcata
     1441 gatatattgc cctaagccat ggagccatat cctaggaaaa tgtctaacag cttcattctt
     1501 aagttcttct aaaagctcta gtgtccattc attgtatggc tccctctgtg gcccttggtc
     1561 ttctggggct tgttccatct atcctctgtt agttttgtaa cactaggcaa aggtggcttt
     1621 atcctttttg gtgttattaa tgctgctagt gctaggtact gtagagatcc taccttgtta
     1681 tgtcctgctt gatattcaca cctaggacta actatatgtc ctaatatggc atttcttatg
     1741 gcagattctg aaaaacaatc aaagtaatac agatgaatta gttggtctgc taggtcaggg
     1801 tctacttgtg tgctatatct ctttttcctc cattctacgg agactccatg acccaaatgc
     1861 caatctcttt ctcctgtatg cagaccccaa tatgttgtta ttaccaatct agcatcccct
     1921 agtgggatgt gtacttctga acttattttt gggtgagtgc tttcatagtg atgtctataa
     1981 aaccatccct ttgctttcct tgaaatatac atatggtgtt ttactaaact tatccatgtt
     2041 ctaatcctca tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc
     2101 tctaatgatc tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac
     2161 tactgcccct tcacctttcc agagaagctt tgctggtcct ttccaaagtg gatctttgtt
     2221 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta gttctttagt
     2281 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     2341 cccttttctt ttaaaattgt ggataaatac tgccatttgt actgctgttt taagatgttc
     2401 agcctgatct cttacctgtc ctataatttt ctttaattct ttattcatag attctactac
     2461 tccttgactt tggggattgt agggaatgcc aaattcctgc ttgatccctg cccaccaaca
     2521 ggcggcctta accgtggtac tggtgaaatt gctgccattg tctgtatgta ttgtttttac
     2581 tggccatctt cctgctaatt ttaagagaaa gtatgctgtt tcctgccctg tctctgctgg
     2641 aataacttct gcttctatat atccactggc tacatgaacc gccaccagga taatttttcc
     2701 ttctagatgt gtgcaatcta gttgccatat tcctggacta cagtctactt gtccatgcat
     2761 ggcttctcct tttagctgac atttatcaca gctggctact atttcttttg ctactacagg
     2821 tggtaagtta aaatcactag ccatggctct ccaattactg tgatatttct catgttcttc
     2881 ttgggcctta tctattccat ctaaaaatag tactttcctg accccagcac tgactaattt
     2941 atctacttgt tcatttcctc caattccttt gtgtgctggt acccatgcca ggtagacctt
     3001 ttcctttttt attaactgct ctattatttg actgactaat tctgattcac ttttatctgg
     3061 ttgtgcttga atgattccta atgcatattg tgagtctgtt actatgttta cttctaatcc
     3121 agaatcctgc aaagctagat gaattgcttc taactcagtc ttctgatttg ttgtgtcagt
     3181 tagggtgaca actttttgtc ttcctttgtt agtaacatat cctgcttttc ctaatttagt
     3241 ctccctgtta gctgctccat ctacatagaa agtttctgcg ccttctatgg gctctttctc
     3301 taactggtac cataatttca ctaagggagg ggtattgaca aactcccatt caggaatcca
     3361 ggtggcttgc caatactctg tccaccatgt ttcccatgtt tctttttgta tgggcagtct
     3421 aaatttagga gtttttcccc atattattat gcattctgtg gttatttttt gcactgcctc
     3481 tgttaattgt tttacatcat tagtgtgggc acccctcgtt cttgcatatt ttcctgtttt
     3541 cagattttta aatggctctt gataaatttg atatgtccat tggccttgcc cctgtttctg
     3601 tatttctgct attaagtctt ttgatgggtc ataatacact ccatgtactg gttcttttag
     3661 aatttccctg ttttctgcca gttctagctc tgcttcttct gttagtgtta ctacttctgt
     3721 tagtgctttg gttcccctaa ggagtttaca taactgcttc actttgatcc ctgagtaaat
     3781 ctgacttgcc caattcaatt ttcccactaa cttctgtatg tcattgacag tccagctgtc
     3841 tttttctggc agcactatag gctgtactgt ccatttatca ggatggagtt cataacccat
     3901 ccaaaggaat ggaggttctt tctgatgttt tttgtctggt gtggtaaatc cccaccttaa
     3961 cagatgttgt ctcagttcct ctatttttgt tctatgctgc cctatttcta agtcagatcc
     4021 tacatacaaa tcatccatgt actgatagat aactatgtct ggattttgct ttctaaaagg
     4081 ctctaagatt tttgtcatgc tactttggaa tattgctggt gatcctttcc atccctgtgg
     4141 gagcacattg tactgatatc taatccctgg tgtctcattg tttatactag gtatggtaaa
     4201 tgcagtatat ttcctgaagt ctttatctaa gggaactgag aaatatgcat cacccacatc
     4261 tagtactgtt actgattttt tcttttttaa tccagcggga tgtggtattc ctaattgaac
     4321 ttcccagaag tcttgagtcc tcttattaag ttctctgaaa tctactaatt ttctccattt
     4381 agtactgtct tttttcttta tagcaaatac tggagtattg tatggatttt caggcccaat
     4441 ttttgaaatc ttcccttcct tttccatttc tgtacaaatt tctactaatg cttttatttt
     4501 ttcttctgtc aatggccatt gtttaacttt tgggccatcc attcctggct ttaattttac
     4561 tggtacagtt tcaataggac taatgggaaa atttagagtg caaccaatct gagtcaacag
     4621 atttcttcca attatgttga caggtgtagg tcctactaat actgtaccta tagctttatg
     4681 tccacagatt tctatgggta tctgatcata ctgtcttact ttgataaaac ctccaattcc
     4741 ccctatcatt tttggcttcc atctccctgg caaattcatt tcttctaata ctgtatcatc
     4801 tgctcctgta tctaatagag cctcctttag ttgcccccct atctttattg tgacgagggg
     4861 tcgttgccaa agagtgatct gagggaagct aagggataca tttccttgtc ttcccgctcc
     4921 tgcttctgag ggggagttgt tgtctcctcc ccaaacctga agctctcttc tggtggagct
     4981 gttggctcta gtctgctctg aggaaaattc cctggccttc ccttgtggga aggccagatc
     5041 ttccctaaaa aattagcctg tctctctgta caatctttca tttggtgtcc ttcctttcca
     5101 catttccagc agcccctttt cctaggggcc ttgcaatttc tggctatatg cccttctttg
     5161 ccacaattga aacacttaac agtctttctt tggttcctaa aattgcctct ctgcatcatt
     5221 atggtggctg aatttgttac ttggctcatt gcttccgcca aaactcttgc tttgtggccg
     5281 ggtcctccca ctccctgaca tgctgtcatc atttcctcta gtgtagctcc tggtcccaat
     5341 gcttttaaaa tggtcttaca atctgggttc gcattttgga ccaacaaggt ttctgtcatc
     5401 caatttttta cctcctgtga agcttgctcg gctcttaaag ttttatagaa cctgtctaca
     5461 tagtctctaa aggattcctt tggtccttgt cttatgtcca gaatgctaga aggactatac
     5521 atccttacta ttttatttaa tcccaggatt atccatcttt tatagatttc tcctactggg
     5581 ataggtggat tatttgtcat ccatcctatt tgttcctgaa gggtactagt agttcctgct
     5641 atgtcacttc cccttggttc tctcatctgg cctggtgcaa caggccctgc ttgcactgga
     5701 tgcaatctat cccattctgc agcttcctcg tcgatggtct cttttaacat gtgcatagct
     5761 gcttgatgtc cccccactgt gtttagcatg gtgtttaaat cttgtggggt ggctccttct
     5821 gataatgctg aaaacatggg tattacttct gggctgaaag ccttctcttc tactactttt
     5881 acccatgcat ttaaagttct gggtgatatg gcctgatgta ccatttgccc ctggatgttc
     5941 tgcactatag ggtaatttcg gctgacctgg ctgctgtttc ctgtgtcagc tgctgcttgc
     6001 tgtgcttttt tcttactgtt gttttgctct tcctctatct tctctaaagc ttccttggtg
     6061 tcttttacat ctatcatttg atgcacacaa tagaggactg ctattgtatt atataatgat
     6121 ttaagttctt ctgatcctgt ctgaagggac ggttgtagct gtcccagtat ttgtctacag
     6181 ccttctgctg tttctaacag gccgggatta actgcgaatc gttctagctc cctgcttgcc
     6241 catactagat gttttaattt atattttttc tttccccctg gccttaaccg aattttctcc
     6301 cacttatcca attttccccc gctcaatact gacgctctcg cacccatctc tctccttcta
     6361 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     6421 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttct ctttcgcttt
     6481 ccggtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtatgagg
     6541 gatctctagc taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     6601 aagctttatt gaggcttaag cagtgggttc cctagctagc cagagagctc ccaggctcag
     6661 atctggtcta accagagaga cccagtacag gcaaaagcag ctgcttatat gcagcatctg
     6721 agggctcgcc actccccggt cccgcccagg ccacgcctcc cctggaaagt ccccagcgga
     6781 aagtcccttg tagaaagcgc gatgtcatcg gttcttgtag tactccggat gcagctctcg
     6841 ggccatgtga tgaaatgcta ggcggctgtc aaacctccac tctagcactt ctctctccgg
     6901 gtcatccatc ccatgctggc tcatagggtg tagcaagctg ttgttttctc cttcattggc
     6961 ctcttctatc ttctctggat caactggtac tagcttgaaa caccatccaa aggtcagtgg
     7021 atatctgatc cctggccctg gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg
     7081 gtaggtccac agatcaagga tatcttgtct tctttgggag ataattagcc cttccaattt
     7141 tctagttatt tttatttact ataatacaaa caacatattc aattattaaa atcaatgata
     7201 tttgctgagg ctttgttcat ggccctagct tatggtcaat tctagtacaa tttccatgtg
     7261 caagggaaaa gatggttaat accaccatta atagtttcaa tgttctacgt atgacaatta
     7321 gatcaagcct gctaattgtg atgtatgtat gtcctatata tgtgcatcca ttgtgagggc
     7381 atatttgtct gtttctttta gttcaaaata aaatttttgg tttatagatg tttgggctat
     7441 tttactgggt acacataaag gattgtggta actctggtgg atcaatcccc ttacactata
     7501 aaatgtagtt ctctttttct actactgttt attgtattaa agtctccttt gcctgattgg
     7561 tatagccaca cttatttcct tcagttagca tttggatggt ataccttttc gcatcatttt
     7621 ctctcaatct ttctaggtac ttatatttag gtaattatta tttgtccagt ctgatgattt
     7681 ttgccattta cttgaaatat ttagcccatt tccatgtaac ataattacta atttagttgc
     7741 ctgcttgttt tatattttct ttcttgtctt cttctgaatt tatcacatat tctatatcca
     7801 atttttttct gtattcattt gttacacatt cttttctttt agtggttacc ctagatattt
     7861 tcttattcat ccttcactta acacaatttt catacatata cttttactac ttcctctata
     7921 atgccagaat catagaacac tgctgagtag cagttttgct atgaaggaga ctacctctgg
     7981 tttgtccctg ttcctaggat gagactctgc agagccttca tttcaaaagc tagcagatcg
     8041 ttatcttcta ggtacctaga tactataaga attgctttgt ttttaagaga cttctggttt
     8101 agctctctag cctaagcagc ttcagaattt ggcaaatgtc tacgggggaa gaaactgggt
     8161 gtgttgaaaa tcatcagtgt gtattcacca cttcctcaaa cgctttcaac tctcacctat
     8221 tgttctcaag tcgcttcccc tagagaatct ttatctcaac accataggac tagaaaatat
     8281 cccaatctcc ttttctgaga ttttggttta tctcttccgc ccagctgaac tacagaattt
     8341 aggaatatac tgagggcgtt ctctgtgttt aaaggcccct catatttcca atctgccact
     8401 caggctgcag cccaaaggcc tcctctactc tgtacccagg aaaaacaatg atcagctgca
     8461 gacctctcag ctcacttgtg aaaggtattc ccccagggtg caaaattttg ctttctttaa
     8521 tcatttttac acccattgtt ctctatcgct aaatatataa attttgtaaa ttacctggct
     8581 tttctagttg ttttattaag agtcttggac taccaagaac tactgcatcc tatcccccaa
     8641 aacgttctaa ttccatttga atagtaagtc ttccacaact ataaatctat gagttgaaat
     8701 aatttgttga tgactttaat ttcaaaataa gacacaagat cacttaccac ttgaggacag
     8761 agatggagag ttaagatttg agaacaggaa aaaatagaga aacgtagccc tgagaaaatc
     8821 aaccattatg aaacaacaga aaacgtgtca gttatatatt atcagactat atatttaaaa
     8881 agtattccat agaaggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8941 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     9001 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
     9061 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     9121 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9181 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9241 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9301 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9361 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9421 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9481 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     9541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9781 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9841 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     9901 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9961 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10021 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10081 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10141 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10201 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10261 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10321 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10381 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10441 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10501 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    11521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    11581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    11641 caatttcaca caggaaacag ctatgaccat gattacgcca agctctaata cgactcacta
    11701 tagggaaagc ttgcatgcct gcaggtcgac tctagaggat ccgtagtgat gtgacatttt
    11761 tcaattccta ttatttaaat ttatgttctc tctccccctc cccatcctcc ttttatcagt
    11821 tttgctaagt ggtcaattac attaatcttt tcaaagaacc aactttttct tagctttgtc
    11881 aattttctct atgtaagatt actttctatt cactaatttc tgctcttgtc ttcattattt
    11941 ctttacttct actgtcttta ggtttgattt cctattcctt ttctacctcc ttaaaaaagc
    12001 tttcttttcc aatgcataga cttaaggcta taaatttcat cttagcattt cagcagcagc
    12061 aatctagatc attgtagttc accttcattt tcaatcagtt taaaatattt tctaatagcc
    12121 atcataaatt cttcttttaa gtcatatgtt attcaaaagc atagtgtttt atttctaggc
    12181 agctaggaat ttttgctaga gattttccac actgactaaa agggtatgag ggatctctag
    12241 ctaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
    12301 tgaggcttaa gcagtgggtt ccctagctag ccagagagct cccaggctca gatctggtct
    12361 aaccagagag acccagtaca ggcaaaagca gctgcttata tgcagcatct gagggctcgc
    12421 cactccccgg tcccgcccag gccacgcctc ccctggaaag tccccagcgg aaagtccctt
    12481 gtagaaagcg cgatgtcatc ggttcttgta gtactccgga tgcagctctc gggccatgtg
    12541 atgaaatgct aggcggctgt caaacctcca ctctagcact tctctctccg ggtcatccat
    12601 cccatgctgg ctcatagggt gtagcaagct gttgttttct ccttcattgg cctcttctat
    12661 cttctctgga tcaactggta ctagcttgaa acaccatcca aaggtcagtg gatatctgat
    12721 ccctggccct ggtgtgtagt tctgccaatc agggaagtag ccttgtgtgt ggtaggtcca
    12781 cagatcaagg atatcttgtc ttctttggga gaaaattagc ccttccagtc cccccttttc
    12841 ttttaaaaag tggctaatat ctacagctgc cttgtaagtt attggtctta aaggtacctg
    12901 aggtctgact ggaaaaccca cctcctcctc ctcttgtgct tccagccagg cacaatcagc
    12961 attgttagct gctgtattgc tacttgtgat tgctccatgt ttttccaggt ctctagatac
    13021 tgctcccacc ccatctgctg ctggctcagc tcgtctcatt ctttccctta cagcatgcca
    13081 tccaccctcc atacgttttg accacttgcc acccatctta tagcaaaagc ctttccaagc
    13141 cctgtcttat tcttctaggt atgtggagaa tagctctata agttctttgt actacttcta
    13201 taaccctatc tgttccctca gctactgcta tggctgtgac attaagcaag ttaacagcac
    13261 tattctttag ttcctggctc caatactgta ggagactcca ccaatacttg agggcttccc
    13321 accccctgcg tcccagaagt tccacaatcc tcgttacaac caagattaag tctctcaagc
    13381 ggtggtagct gaagaggcac aggctccgca gatcgtccca gaaaagtgct aagaatccgt
    13441 tcactaatcg accggatctg tctctgtctc tctctccacc ttcttcttcg attccttcgg
    13501 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgacaatggt gagtatccct
    13561 gcctaactct attcactata gaaagtacag taaaaactat tcttaaacct atcaagcctc
    13621 ctactatcat tatgaatatt tttatatacc acagccagtt tgttattgta aaccaattcc
    13681 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggttct
    13741 gcgattcttc aattaagttg tatattaagt ctgtgtaatt ttcaatttct ctttcccact
    13801 gcatccaagt catgttatcc caaatctgat tcagagattt attactccaa ctagcattcc
    13861 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
    13921 gatcctttag gtatctttcc acagccagga ctcttgcctg gagctgcttg atgccccaga
    13981 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgtt
    14041 gcactatacc agacaataat tgtctggcct gtaccgtcaa cgtcattgac gctgcgccca
    14101 tagtgcttcc tgctgctccc aagaacccaa ggaacagagc tcctattccc actgctcttt
    14161 tttctctttg caccactctt ctctttgccg tggtgggtgc tactcctaat ggttcaattt
    14221 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
    14281 gtctgaaggt ctcggtggta ttggtatcgc tcgtaccacc atctcttgtt aatagcagcc
    14341 ctgtaatatt tgatgtacat ctaatttgtc cgctgatggg aggggcatac attgcttttc
    14401 ctacttcctg ccacatgttt ataatttgtt ttattctgca tgggagtgtg atatttcctc
    14461 cagtgtcatc tgacctttca gcactactat tccaagtatt attcccagcc caagtactat
    14521 taaacagttg tgttgactta cagtagaaaa attcccctcc acaattaaaa ctatgcatta
    14581 caatttctgg gtcccctcct gaggattgat taaagactat tgttttattc tcaaattgtt
    14641 ctcttaattt tataactatc tgtcttaaag tgtcattcca ttttgctcta ctaaggttac
    14701 aatgtgcttg tcttatatct cctactattt ctcctgttgt ataatatact ctccctggtc
    14761 ccgtagttat acgttttctt gttttttt
//
