LOCUS       sequence-426704-        5918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein RA anchored to cytosolic 
            face of the caveolae with mouse CAVIN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, 
            Zanellati MC, Hsu CH, Marchan MF, Cohen S
  TITLE     Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for
            Visualizing Membrane Contact Site Dynamics.
  JOURNAL   Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 
            10.1177/25152564241228911. eCollection 2024 Jan-Dec.
  PUBMED    38327561
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Contact 
            (Thousand Oaks)."; date: "2024-02-4"; volume: "7:25152564241228911. 
            doi"; pages: " 10.1177/25152564241228911. eCollection 2024 Jan-Dec"
COMMENT     Sequence Label: Cav-RA
FEATURES             Location/Qualifiers
     source          1..5918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      557..566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1905..1923)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2173..2192
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2661..2782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2698..2717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2752..2771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2789..3244)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2926..2947)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3138..3157
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3271..3375
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3377..3734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3377..3568
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3400..3420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3585..3720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3769..4563
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3769..4563
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3823..3842)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4433..4452
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4751..4770)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4795..4842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5171..5759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5660..5679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgtgccgcca ccatggagga tgtcacgctc catatcgttg agcggccgta
      601 ttccggattt cccgatgctt cctcagaggg cccggagccc acccaagggg aggcgcgggc
      661 cacggaggag ccgtcgggga ccggctccga cgagctgatc aagtcggacc aggtgaacgg
      721 tgtgctggtg ctgagccttc tggataaaat catcggcgcc gttgaccaga tccagctgac
      781 ccaagcccag ctggaggagc gacaggcgga gatggagggc gctgtgcaga gcatccaggg
      841 agagctgagc aagctgggca aggcgcacgc caccacgagc aacaccgtga gcaagttgct
      901 ggagaaggtg cgcaaggtca gcgtcaacgt gaagaccgtg cgcggcagcc tggagcgcca
      961 ggccggccag ataaagaaac tggaggtcaa cgaggcggag ctgctgaggc gccgcaactt
     1021 caaagtcatg atctaccagg atgaagtcaa gctgccggcc aaactgagcg tcagcaagtc
     1081 gctgaaagag tcggaggcac tgcctgagaa ggagggtgac gagctgggcg agggcgagcg
     1141 gcccgaggat gacaccgcgg cgatcgagct gtcgtccgac gaggcggtgg aggtggagga
     1201 ggtgatcgag gagtcccgcg ccgagcgcat caagcgcagc ggcctgcggc gcgtggacga
     1261 cttcaagaag gccttctcca aggagaagat ggagaagacc aaggtgcgca cgcgtgagaa
     1321 cctggagaag acgcgcctga agaccaagga gaacctggag aagacacggc acacgctgga
     1381 gaagcgcatg aacaagctgg gcacgcgcct ggtgcccgtg gagcgacgag agaagctgaa
     1441 gacatcccgg gacaagctgc gcaagtcctt cacgcccgac catgtggtgt atgcgcgctc
     1501 caagaccgct gtctacaagg tgccgccttt caccttccac gtcaagaaga tccgcgaggg
     1561 cgaggtggag gtgctgaagg ccaccgagat ggtggaggtg ggtcccgagg acgacgaggt
     1621 tggcgcggag cgcggcgagg ccactgacct gctgcgcggg agcagccccg acgtgcacac
     1681 gctgctggag atcaccgagg agtcggacgc cgtcctggtg gacaagagcg acagcgacgg
     1741 tggtggtggt agtggtggtg gtggtagtgc tatggtgagc aagagcgagg aggtcatcaa
     1801 agagttcatg cgcttcaagg tgcgcttgga gggctccatg aacggccacg agttcgagat
     1861 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1921 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagatca tgtacggctc
     1981 caaggcgtac gtgaagcacc ccgccgacgt ccccgattac atgaagctgt ccttccccga
     2041 gggcttcaag tgggagcgcg tgatgcactt cgaggacggc ggtctggtga ccgtgactca
     2101 ggacacctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     2161 cccccccgac ggccccgtaa tgcagaagaa gaccttgggc tgggattatg ccaccgagcg
     2221 cctgtacccc gaagacggcg tgctgaaggg cgagcttctg gggcgcctga agctgaagga
     2281 cggcggcctc aacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     2341 gcccggctac tacttcgtgg acaccaagct gggcatcacc tcccacaacg aggactacac
     2401 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgggcatgg acgagctgta
     2461 caagggtacc taatagaagc ttggtaaacc gctgatcagc ctcgactgca gtcgacggta
     2521 ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     2581 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2641 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2701 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2761 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     2821 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2881 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2941 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3001 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3061 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3121 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3181 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3241 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3301 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3361 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3481 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3541 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3601 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3661 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3721 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3781 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3841 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3901 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3961 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4021 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4081 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4141 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4201 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4261 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4321 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4381 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4441 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4501 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4561 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4621 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4681 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4741 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4801 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     4861 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4921 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     4981 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     5041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     5881 taatagtaat caattacggg gtcattagtt catagccc
//
