LOCUS       sequence-426709-        6319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length human PQBP1 from a His-SUMO bacterial 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, 
            Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI,
            Johnson JS
  TITLE     Cell-free assays reveal that the HIV-1 capsid protects reverse 
            transcripts from cGAS immune sensing.
  JOURNAL   PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 
            10.1371/journal.ppat.1012206.
  PUBMED    39874383
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2025-01-28"; volume: "21(1):e1012206. doi"; pages: 
            " 10.1371/journal.ppat.1012206"
COMMENT     Sequence Label: pCA528 huPQBP1
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(153..172)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             164..979
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             164..979
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(237..256)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(1071..1526)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1208..1229)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1420..1439
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(1563..1610)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1606..1624
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(2472..2762)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(2766..2783)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             2797..2819
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    2834..2858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(2858..2877)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2859..2877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2961..2980)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3186..3263
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3264..4346
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3283..3302)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             5155..5346
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5340..5362)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5448..5587
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5503..5522
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5602..5619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5773..6319,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5853..5872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac
      121 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
      181 ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
      241 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
      301 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
      361 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
      421 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc
      481 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
      541 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
      601 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
      661 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
      721 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
      781 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
      841 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
      901 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
      961 gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga
     1021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     1081 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     1141 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     1201 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     1261 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     1321 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     1381 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     1441 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     1501 gcttaatgcg ccgctacagg gcgcgtccca ttcgccaatc cggatatagt tcctcctttc
     1561 agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca
     1621 gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg atctcaatcc
     1681 tgctgttttg tacgactcgc ttccgcgttt gcacgaagga cagcgccagg tgaaggatac
     1741 ggacgctgtt ggaacaaagg cccggccgct gtcgtgtctg cgcctgtctt tgcttcgttg
     1801 cgcttcggaa gtccagtgga ccaggtgcca cgcggagcat cggaataaga gctagggtcc
     1861 atcgggtcta attcttcgtc cttgcgggac accgcctttt tagatttagg atacggagct
     1921 aattcctcgc gacggtggtg gcgacgttcc ttcccctcct cacggtcagc cttgtcgtat
     1981 ccgcgatcac gatcgcgttc acggtcgcgc tcacgctcac gatcgacttt gtcatacccg
     2041 cgttcgcggt cacgatcact cttatcatgt ccgcggtcgg acttgtcatg cccgcggtca
     2101 agcttttcgt ggctacgatc gcttttgtca tgaccgcggt cacttttatc atgggagcga
     2161 tcaagctttt cttccgcatc cgcgttagat gagcgcagtt tctttgctga ttttgttact
     2221 acactgtttg gatcgtgagg gctcagccaa ctgactaaat ctgtatcggc attccaatag
     2281 tacggaagcc cgcaagacgg gtcgaagact ttgtaccatg aaggcggcag cccctctaag
     2341 cgggtggcct cgtagtccac cgggtcatca tcgtagtctt cggcaatgat ctcctcttca
     2401 ggctccggtt ccaaatgctt caaaatccca cgcttggcca ggcgggtctg taaggctacg
     2461 ggtaacggca tcccaccaat ctgttctctg tgagcctcaa taatatcgtt atcctccatg
     2521 tccaaatctt caggggtctg atcagcttga attctaatac cgtcgtacaa gaatcttaag
     2581 gagtccattt ccttaccctg tcttttagcg aacgcttcca tcagccttct taaaggagtg
     2641 gtctttttga tcttgaagaa gatctctgaa gatccatcgg acacctttaa attgatgtga
     2701 gtctcaggct tgacttctgg cttgacctct ggcttagctt cttgattgac ttctgagtcc
     2761 gaaccatggt gatgatggtg atgacccata tgtatatctc cttcttaaag ttaaacaaaa
     2821 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
     2881 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     2941 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     3001 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     3061 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     3121 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga
     3181 tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
     3241 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     3301 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     3361 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     3421 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     3481 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     3541 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     3601 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     3661 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     3721 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
     3781 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     3841 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     3901 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     3961 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     4021 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     4081 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
     4141 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     4201 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     4261 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4321 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat
     4381 taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     4441 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     4501 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     4561 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     4621 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     4681 gcggccctac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     4741 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     4801 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     4861 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     4921 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     4981 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     5041 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     5101 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     5161 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     5221 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     5281 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     5341 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5401 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     5461 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     5521 tactgagagt gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5581 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6181 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6301 tggtagcggt ggttttttt
//
