LOCUS       sequence-42671-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, 
            Heliot L, McNally JG
  TITLE     Concurrent fast and slow cycling of a transcriptional activator at 
            an endogenous promoter.
  JOURNAL   Science. 2008 Jan 25;319(5862):466-9.
  PUBMED    18218898
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2008-01-25"; volume: "319(5862)"; pages: "466-9"
COMMENT     Sequence Label: pTSK368
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..778)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cagccgcaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatccgcg
       61 gttatttgta caattcatcc ataccatggg taataccagc agcagtaaca aattctaaca
      121 agaccatgtg gtctctcttt tcgtttggat ctttggataa ggcagattga gtggataagt
      181 aatggttgtc tggtaacaag actggaccat caccaattgg agtattttgt tgataatggt
      241 cagctaattg aacagaacca tcttcaatgt tgtgtctaat tttgaagtta actttgatac
      301 cattcttttg tttgtcagcc atgatgtaaa cattgtgaga gttatagttg tattccaatt
      361 tgtgacctaa aatgttacca tcttctttaa aatcaatacc ttttaattcg attctattaa
      421 ctaaggtatc accttcaaac ttgacttcag ctctggtctt gtagttaccg tcatctttga
      481 aaaaaatagt tctttcttga acataacctt ctggcatggc agacttgaaa aagtcatgtt
      541 gtttcatatg atctgggtat ctcgcaaaac attgaacacc ataaccgaaa gtagtgacta
      601 aggttggcca tggaactggc aatttaccag tagtacaaat aaattttaag gtcaatttac
      661 cgtaagtagc atcaccttca ccttcaccgg agacagaaaa tttgtgacca ttaacatcac
      721 catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct ttagacattc
      781 tagatttgta caattcatcc ataccatggg taataccagc agcagtaaca aattctaaca
      841 agaccatgtg gtctctcctt tcgtttggat c
//
