LOCUS       sequence-426714-        5739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV reporter genome plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Huang Q, Chan KY, Wu J, Botticello-Romero NR, Zheng Q, Lou S, Keyes 
            C, Svanbergsson A, Johnston J, Mills A, Lin CY, Brauer PP, Clouse G,
            Pacouret S, Harvey JW, Beddow T, Hurley JK, Tobey IG, Powell M, Chen
            AT, Barry AJ, Eid FE, Chan YA, Deverman BE
  TITLE     An AAV capsid reprogrammed to bind human transferrin receptor 
            mediates brain-wide gene delivery.
  JOURNAL   Science. 2024 Jun 14;384(6701):1220-1227. doi: 
            10.1126/science.adm8386. Epub 2024 May 16.
  PUBMED    38753766
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adm8386"; journalName: "Science"; date: 
            "2024-06-14- 14"; volume: "384"; issue: "6701"; pages: "1220-1227"
COMMENT     Sequence Label: pAAV-CMV-CBA-hGBA1-HA-hGHpA
FEATURES             Location/Qualifiers
     source          1..5739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        210..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..792
                     /label=chicken beta-actin promoter
     regulatory      808..817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2422..2448
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2486..2962
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2685..2704)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3002..3142
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3002..3131
                     /label=AAV2 ITR
     rep_origin      3217..3672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3304..3323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3514..3535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3689..3708)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3808..3830
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3868..3886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3954..4058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4059..4127,4128..4919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4277..4296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5090..5678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5579..5598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgcggcccaa cggtaccgcc accatggagt tttcaagtcc ttccagagag
      841 gaatgtccca agcctttgag tagggtaagc atcatggctg gcagcctcac aggattgctt
      901 ctacttcagg cagtgtcgtg ggcatcaggt gcccgcccct gcatccctaa aagcttcggc
      961 tacagctcgg tggtgtgtgt ctgcaatgcc acatactgtg actcctttga ccccccgacc
     1021 tttcctgccc ttggtacctt cagccgctat gagagtacac gcagtgggcg acggatggag
     1081 ctgagtatgg ggcccatcca ggctaatcac acgggcacag gcctgctact gaccctgcag
     1141 ccagaacaga agttccagaa agtgaaggga tttggagggg ccatgacaga tgctgctgct
     1201 ctcaacatcc ttgccctgtc accccctgcc caaaatttgc tacttaaatc gtacttctct
     1261 gaagaaggaa tcggatataa catcatccgg gtacccatgg ccagctgtga cttctccatc
     1321 cgcacctaca cctatgcaga cacccctgat gatttccagt tgcacaactt cagcctccca
     1381 gaggaagata ccaagctcaa gatacccctg attcaccgag ccctgcagtt ggcccagcgt
     1441 cccgtttcac tccttgccag cccctggaca tcacccactt ggctcaagac caatggagcg
     1501 gtgaatggga aggggtcact caagggacag cccggagaca tctaccacca gacctgggcc
     1561 agatactttg tgaagttcct ggatgcctat gctgagcaca agttacagtt ctgggcagtg
     1621 acagctgaaa atgagccttc tgctgggctg ttgagtggat accccttcca gtgcctgggc
     1681 ttcacccctg aacatcagcg agacttcatt gcccgtgacc taggtcctac cctcgccaac
     1741 agtactcacc acaatgtccg cctactcatg ctggatgacc aacgcttgct gctgccccac
     1801 tgggcaaagg tggtactgac agacccagaa gcagctaaat atgttcatgg cattgctgta
     1861 cattggtacc tggactttct ggctccagcc aaagccaccc taggggagac acaccgcctg
     1921 ttccccaaca ccatgctctt tgcctcagag gcctgtgtgg gctccaagtt ctgggagcag
     1981 agtgtgcggc taggctcctg ggatcgaggg atgcagtaca gccacagcat catcacgaac
     2041 ctcctgtacc atgtggtcgg ctggaccgac tggaaccttg ccctgaaccc cgaaggagga
     2101 cccaattggg tgcgtaactt tgtcgacagt cccatcattg tagacatcac caaggacacg
     2161 ttttacaaac agcccatgtt ctaccacctt ggccacttca gcaagttcat tcctgagggc
     2221 tcccagagag tggggctggt tgccagtcag aagaacgacc tggacgcagt ggcactgatg
     2281 catcccgatg gctctgctgt tgtggtcgtg ctaaaccgct cctctaagga tgtgcctctt
     2341 accatcaagg atcctgctgt gggcttcctg gagacaatct cacctggcta ctccattcac
     2401 acctacctgt ggcgtcgcca gtatccatat gacgtcccag actatgcata gaattcgata
     2461 tcaaagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     2521 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     2581 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     2641 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     2701 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     2761 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     2821 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     2881 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     2941 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     3001 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3061 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     3121 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3181 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     3241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     3301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3421 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3541 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     3601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3661 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3721 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3841 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5701 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
