LOCUS       sequence-426731-       11711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of EGF, the plasmid encodes a recombinant EGF 
            fused extracellularly to the mScarlet fluorescent protein and 
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBbleo-EGF-ScNeo
FEATURES             Location/Qualifiers
     source          1..11711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1668
                     /label=chicken beta-actin promoter
     intron          1669..2685
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2609..2631
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2693..2712
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5545..5571
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5572..6267
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5705..5723)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5973..5992
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7829..8378
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7973..7990)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8200..8219
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8381..8755
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(8795..8812)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8801..9025
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9072..9088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9096..9112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9120..9126,9127..9144,9145..9150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9165..9186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   9420..9482
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9523..9543)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9523..9541)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9562..9578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9586..9602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9610..9616,9617..9634,9635..9640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9655..9676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9793..9810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9964..10552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10044..10063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10723..11514,11515..11583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11346..11365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11584..11688)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1741 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1801 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1861 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1921 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1981 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2041 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2101 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2161 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2221 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2281 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2341 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2401 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2461 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2521 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2581 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2641 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2701 ctggttattg tgctgtctca tcattttggc aaagaattgg gatccgaatt caccatgctg
     2761 ctcactctta tcattctgtt gccagtagtt tcaaaattta gttttgttag tctctcagca
     2821 ccgcagcact ggagctgtcc tgaaggtact ctcgcaggaa atgggaattc tacttgtgtg
     2881 ggtcctgcac ccttcttaat tttctcccat ggaaatagta tctttaggat tgacacagaa
     2941 ggaaccaatt atgagcaatt ggtggtggat gctggtgtct cagtgatcat ggattttcat
     3001 tataatgaga aaagaatcta ttgggtggat ttagaaagac aacttttgca aagagttttt
     3061 ctgaatgggt caaggcaaga gagagtatgt aatatagaga aaaatgtttc tggaatggca
     3121 ataaattgga taaatgaaga agttatttgg tcaaatcaac aggaaggaat cattacagta
     3181 acagatatga aaggaaataa ttcccacatt cttttaagtg ctttaaaata tcctgcaaat
     3241 gtagcagttg atccagtaga aaggtttata ttttggtctt cagaggtggc tggaagcctt
     3301 tatagagcag atctcgatgg tgtgggagtg aaggctctgt tggagacatc agagaaaata
     3361 acagctgtgt cattggatgt gcttgataag cggctgtttt ggattcagta caacagagaa
     3421 ggaagcaatt ctcttatttg ctcctgtgat tatgatggag gttctgtcca cattagtaaa
     3481 catccaacac agcataattt gtttgcaatg tccctttttg gtgaccgtat cttctattca
     3541 acatggaaaa tgaagacaat ttggatagcc aacaaacaca ctggaaagga catggttaga
     3601 attaacctcc attcatcatt tgtaccactt ggtgaactga aagtagtgca tccacttaca
     3661 caacccaagg cagaagatga cacttgggag cctgatgtta atgaatgtgc tttttggaat
     3721 catggctgta ctcttgggtg taaaaacacc cctggatcct attactgcac gtgccctgta
     3781 ggatttgttc tgcttcctga tgggaaacga tgtcatcaac ttgtttcctg tccacgcaat
     3841 gtgtctgaat gcagccatga ctgtgttctg acatcagaag gtcccttatg tttctgtcct
     3901 gaaggctcag tgcttgagag agatgggaaa acatgtagcg gttgttcctc acccgataat
     3961 ggtggatgta gccagctctg cgttcctctt agcccagtat cctgggaatg tgattgcttt
     4021 cctgggtatg acctacaact ggatgaaaaa agctgtgcag cttcaggacc acaaccattt
     4081 ttgctgtttg ccaattctca agatattcga cacatgcatt ttgatggaac agactatgga
     4141 actctgctca gccagcagat gggaatggtt tatgccctag atcatgaccc tgtggaaaat
     4201 aagatatact ttgcccatac agccctgaag tggatagaga gagctaatat ggatggttcc
     4261 cagcgagaaa ggcttattga ggaaggagta gatgtgccag aaggtcttgc tgtggactgg
     4321 attggccgta gattctattg gacagacaga gggaaatctc tgattggaag gagtgattta
     4381 aatgggaaac gttccaaaat aatcactaag gagaacatct ctcaaccacg aggaattgct
     4441 gttcatccaa tggccaagag attattctgg actgatacag ggattaatcc acgaattgaa
     4501 agttcttccc tccaaggcct tggccgtctg gttatagcca gctctgatct aatctggccc
     4561 agtggaataa cgattgactt cttaactgac aagttgtact ggtgcgatgc caagcagtct
     4621 gtgattgaaa tggccaatct ggatggttca aaacgccgaa gacttaccca gaatgatgta
     4681 ggtcacccat ttgctgtagc agtgtttgag gattatgtgt ggttctcaga ttgggctatg
     4741 ccatcagtaa taagagtaaa caagaggact ggcaaagata gagtacgtct ccaaggcagc
     4801 atgctgaagc cctcatcact ggttgtggtt catccattgg caaaaccagg agcagatccc
     4861 tgcttatatc aaaacggagg ctgtgaacat atttgcaaaa agaggcttgg aactgcttgg
     4921 tgttcgtgtc gtgaaggttt tatgaaagcc tcagatggga aaacgtgtct ggctctggat
     4981 ggtcatcagc tgttggcagg tggtgaagtt gatctaaaga accaagtaac accattggac
     5041 atcttgtcca agactagagt gtcagaagat aacattacag aatctcaaca catgctagtg
     5101 gctgaaatca tggtgtcaga tcaagatgac tgtgctcctg tgggatgcag catgtatgct
     5161 cggtgtattt cagagggaga ggatgccaca tgtcagtgtt tgaaaggatt tgctggggat
     5221 ggaaaactat gttctgatat agatgaatgt gagatgggtg tcccagtgtg cccccctgcc
     5281 tcctccaagt gcatcaacac cgaaggtggt tatgtctgcc ggtgctcaga aggctaccaa
     5341 ggagatggga ttcactgtct tgatattgat gagtgccaac tgggggtgca cagctgtgga
     5401 gagaatgcca gctgcacaaa tacagaggga ggctatacct gcatgtgtgc tggacgcctg
     5461 tctgaaccag gactgatttg ccctgactct actccacccc ctcacctcag ggaagatgac
     5521 caccactatt ccgtaagaaa tagttaccca tacgatgttc cggattacgc tatggtgagc
     5581 aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg
     5641 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     5701 accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc
     5761 cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat ccccgactac
     5821 tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5881 ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg
     5941 aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc
     6001 tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag
     6061 atggccctgc gcctgaagga cggaggccgc tacctggcgg acttcaagac cacctacaag
     6121 gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc
     6181 tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc
     6241 accggcggca tggacgagct gtacaagggt ggtggtagtg gtggtggttc aaatagtgac
     6301 tctgaatgtc ccctgtccca cgatgggtac tgcctccatg atggtgtgtg catgtatatt
     6361 gaagcattgg acaagtatgc atgcaactgt gttgttggct acatcgggga gcgatgtcag
     6421 taccgagacc tgaagtggtg ggaactgcgc cacgctggcc acgggcagca gcagaaggtc
     6481 atcgtggtgg ctgtctgcgt ggtggtgctt gtcatgctgc tcctcctgag cctgtggggg
     6541 gcccactact acaggactca gaagctgcta tcgaaaaacc caaagaatcc ttatgaggag
     6601 tcgagcagag atgtgaggag tcgcaggcct gctgacactg aggatgggat gtcctcttgc
     6661 cctcaacctt ggtttgtggt tataaaagaa caccaagacc tcaagaatgg gggtcaacca
     6721 gtggctggtg aggatggcca ggcagcagat gggtcaatgc aaccaacttc atggaggcag
     6781 gagccccagt tatgtggaat gggcacagag caaggctgct ggattccagt atccagtgat
     6841 aagggctcct gtccccaggt aatggagcga agctttcata tgccctccta tgggacacag
     6901 acccttgaag ggggtgtcga gaagccccat tctctcctat cagctaaccc attatggcaa
     6961 caaagggccc tggacccacc acaccaaatg gagctgactc agggcggatt cgaaatggta
     7021 agcaaggggg aggaagataa tatggcttcc cttccagcaa cgcacgaatt gcatatcttt
     7081 ggaagtatta atggagttga tttcgacatg gtggggcagg gtacgggaaa cccaaacgat
     7141 ggctatgaag aacttaacct caagtctacc aaaggcgatc tccaattctc accctggata
     7201 ttggtcccac acatcggcta cgggttccat caatacctgc cttatcctga cgggatgagt
     7261 ccttttcaag ctgctatggt tgacggaagt ggctaccaag ttcaccgcac aatgcaattt
     7321 gaggatggtg catctcttac ggttaattac cgatatacat atgagggttc acacatcaag
     7381 ggggaagctc aggtcaaggg cactggattt cccgcagatg gcccagttat gaccaattct
     7441 ctgaccgcag ccgattggtg tcgaagcaag aagacctacc ctaacgacaa gactatcatc
     7501 tcaaccttca aatggtccta caccacaggt aacggaaagc ggtataggtc tacagctcgg
     7561 acaacatata cctttgcgaa gcctatggcc gctaactatt tgaaaaacca accgatgtat
     7621 gtttttcgga agaccgaatt gaaacattca aaaacggagt tgaatttcaa agagtggcag
     7681 aaggcattca ccgacgtaat gggtatggat gagttgtata aataagcggc ggccgctcta
     7741 gagtcgacgg gccgcggtaa caattgttaa ctaacttaag ctagcaacgg tttccctcta
     7801 gcgggatcaa ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     7861 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     7921 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     7981 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     8041 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     8101 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     8161 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     8221 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     8281 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     8341 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggccaagt tgaccagtgc
     8401 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     8461 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8521 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8581 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8641 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8701 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgaaagtc
     8761 gagatgcatc gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     8821 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8881 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8941 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     9001 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaacctgcag cccaagcttg
     9061 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     9241 atcttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     9301 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     9361 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
     9421 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
     9481 ggttaatcta gtatacgcgt ttaattaatc cagcttttgt tccctttagt gagggttaat
     9541 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11701 aaaagtgcca c
//
