LOCUS       sequence-42674-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Ding Y, Ai HW, Hoi H, Campbell RE
  TITLE     Forster resonance energy transfer-based biosensors for 
            multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 
            activity in single cells.
  JOURNAL   Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 
            2011 Nov 28.
  PUBMED    22080726
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac202595g";
            journalName: "Anal Chem"; date: "2011-12-15- 15"; volume: "83"; 
            issue: "24"; pages: "9687-93"
COMMENT     Sequence Label: CaYin1 NES
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(222..240)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     490..509
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctagcc gccaccatgg cctcgagcat ggtgagcaag ggcgaggagg tcatcaaaga
      121 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
      181 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      241 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
      301 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
      361 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
      421 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
      481 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
      541 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
      601 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
      661 cggctactac tac
//
