LOCUS       sequence-426750-        9266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) 
            fused with human Ago2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Sekar V, Marmol-Sanchez E, Kalogeropoulos P, Stanicek L, Sagredo EA,
            Widmark A, Doukoumopoulos E, Bonath F, Biryukova I, Friedlander MR
  TITLE     Detection of transcriptome-wide microRNA-target interactions in 
            single cells with agoTRIBE.
  JOURNAL   Nat Biotechnol. 2023 Sep 21. doi: 10.1038/s41587-023-01951-0.
  PUBMED    37735263
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Sep 21. doi: 10.1038/s41587-023-01951-0."
COMMENT     Sequence Label: pcDNA3.1_ADAR2DD_E488Q_Ago2
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4159..4168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4857..4874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4863..5087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5133..5561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5570..5590)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5575..5904
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5755..5890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5817..5836
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5971..6765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6025..6044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6635..6654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6939..7060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6976..6995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7030..7049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7109..7125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7109..7125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7122..7144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7133..7149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7157..7163,7164..7181,7182..7187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7202..7223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7340..7357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7511..8099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7591..7610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8270..9061,9062..9130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8893..8912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9131..9235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgc agctgcattt accgcaggtt ttagctgacg ctgtctcacg cctggtcctg
      961 ggtaagtttg gtgacctgac cgacaacttc tcctcccctc acgctcgcag aaaagtgctg
     1021 gctggagtcg tcatgacaac aggcacagat gttaaagatg ccaaggtgat aagtgtttct
     1081 acaggaacaa aatgtattaa tggtgaatac atgagtgatc gtggccttgc attaaatgac
     1141 tgccatgcag aaataatatc tcggagatcc ttgctcagat ttctttatac acaacttgag
     1201 ctttacttaa ataacaaaga tgatcaaaaa agatccatct ttcagaaatc agagcgaggg
     1261 gggtttaggc tgaaggagaa tgtccagttt catctgtaca tcagcacctc tccctgtgga
     1321 gatgccagaa tcttctcacc acatgagcca atcctggaag aaccagcaga tagacaccca
     1381 aatcgtaaag caagaggaca gctacggacc aaaatagagt ctggtcaggg gacgattcca
     1441 gtgcgctcca atgcgagcat ccaaacgtgg gacggggtgc tgcaagggga gcggctgctc
     1501 accatgtcct gcagtgacaa gattgcacgc tggaacgtgg tgggcatcca gggatccctg
     1561 ctcagcattt tcgtggagcc catttacttc tcgagcatca tcctgggcag cctttaccac
     1621 ggggaccacc tttccagggc catgtaccag cggatctcca acatagagga cctgccacct
     1681 ctctacaccc tcaacaagcc tttgctcagt ggcatcagca atgcagaagc acggcagcca
     1741 gggaaggccc ccaacttcag tgtcaactgg acggtaggcg actccgctat tgaggtcatc
     1801 aacgccacga ctgggaagga tgagctgggc cgcgcgtccc gcctgtgtaa gcacgcgttg
     1861 tactgtcgct ggatgcgtgt gcacggcaag gttccctccc acttactacg ctccaagatt
     1921 accaaaccca acgtgtacca tgagtccaag ctggcggcaa aggagtacca ggccgccaag
     1981 gcgcgtctgt tcacagcctt catcaaggcg gggctggggg cctgggtgga gaagcccacc
     2041 gagcaggacc agttctcact cacgcccgga ggaggtggaa gcggaggagg tggaagcgga
     2101 ggaggtggaa gcggaggagg tggaagcgga ggaggtggaa gcggaggagg tggaagcgga
     2161 ggaggtggaa gcggaggagg tggaagcgga ggaggtggaa gcggaggagg tggaagcgga
     2221 ggaggtggaa gcatgtactc gggagccggc cccgcacttg cacctcctgc gccgccgccc
     2281 cccatccaag gatatgcctt caagcctcca cctagacccg actttgggac ctccgggaga
     2341 acaatcaaat tacaggccaa tttcttcgaa atggacatcc ccaaaattga catctatcat
     2401 tatgaattgg atatcaagcc agagaagtgc ccgaggagag ttaacaggga aatcgtggaa
     2461 cacatggtcc agcactttaa aacacagatc tttggggatc ggaagcccgt gtttgacggc
     2521 aggaagaatc tatacacagc catgcccctt ccgattggga gggacaaggt ggagctggag
     2581 gtcacgctgc caggagaagg caaggatcgc atcttcaagg tgtccatcaa gtgggtgtcc
     2641 tgcgtgagct tgcaggcgtt acacgatgca ctttcagggc ggctgcccag cgtccctttt
     2701 gagacgatcc aggccctgga cgtggtcatg aggcacttgc catccatgag gtacaccccc
     2761 gtgggccgct ccttcttcac cgcgtccgaa ggctgctcta accctcttgg cgggggccga
     2821 gaagtgtggt ttggcttcca tcagtccgtc cggccttctc tctggaaaat gatgctgaat
     2881 attgatgtgt cagcaacagc gttttacaag gcacagccag taatcgagtt tgtttgtgaa
     2941 gttttggatt ttaaaagtat tgaagaacaa caaaaacctc tgacagattc ccaaagggta
     3001 aagtttacca aagaaattaa aggtctaaag gtggagataa cgcactgtgg gcagatgaag
     3061 aggaagtacc gtgtctgcaa tgtgacccgg cggcccgcca gtcaccaaac attcccgctg
     3121 cagcaggaga gcgggcagac ggtggagtgc acggtggccc agtatttcaa ggacaggcac
     3181 aagttggttc tgcgctaccc ccacctccca tgtttacaag tcggacagga gcagaaacac
     3241 acctaccttc ccctggaggt ctgtaacatt gtggcaggac aaagatgtat taaaaaatta
     3301 acggacaatc agacctcaac catgatcaga gcgactgcta ggtcggcgcc cgatcggcaa
     3361 gaagagatta gcaaattgat gcgaagtgca agtttcaaca cagatccata cgtccgtgaa
     3421 tttggaatca tggtcaaaga tgagatgaca gacgtgactg ggcgggtgct gcagccgccc
     3481 tccatcctct acgggggcag gaataaagct attgcgaccc ctgtccaggg cgtctgggac
     3541 atgcggaaca agcagttcca cacgggcatc gagatcaagg tgtgggccat tgcgtgcttc
     3601 gccccccagc gccagtgcac ggaagtccat ctgaagtcct tcacagagca gctcagaaag
     3661 atctcgagag acgccggcat gcccatccag ggccagccgt gcttctgcaa atacgcgcag
     3721 ggggcggaca gcgtggagcc catgttccgg cacctgaaga acacgtatgc gggcctgcag
     3781 ctggtggtgg tcatcctgcc cggcaagacg cccgtgtacg ccgaggtcaa gcgcgtggga
     3841 gacacggtgc tggggatggc cacgcagtgc gtgcagatga agaacgtgca gaggaccacg
     3901 ccacagaccc tgtccaacct ctgcctgaag atcaacgtca agctgggagg cgtgaacaac
     3961 atcctgctgc cccagggcag gccgccggtg ttccagcagc ccgtcatctt tctgggagca
     4021 gacgtcactc acccccccgc cggggatggg aagaagccct ccattgccgc cgtggtgggc
     4081 agcatggacg cccaccccaa tcgctactgc gccaccgtgc gcgtgcagca gcaccggcag
     4141 gagatcatac aagacctggc cgccatggtc cgcgagctcc tcatccagtt ctacaagtcc
     4201 acgcgcttca agcccacccg catcatcttc taccgcgacg gtgtctctga aggccagttc
     4261 cagcaggttc tccaccacga gttgctggcc atccgtgagg cctgtatcaa gctagaaaaa
     4321 gactaccagc ccgggatcac cttcatcgtg gtgcagaaga ggcaccacac ccggctcttc
     4381 tgcactgaca agaacgagcg ggttgggaaa agtggaaaca ttccagcagg cacgactgtg
     4441 gacacgaaaa tcacccaccc caccgagttc gacttctacc tgtgtagtca cgctggcatc
     4501 caggggacaa gcaggccttc gcactatcac gtcctctggg acgacaatcg tttctcctct
     4561 gatgagctgc agatcctaac ctaccagctg tgtcacacct acgtgcgctg cacacgctcc
     4621 gtgtccatcc cagcgccagc atactacgct cacctggtgg ccttccgggc caggtaccac
     4681 ctggtggata aggaacatga cagtgctgaa ggaagccata cctctgggca gagtaacggg
     4741 cgagaccacc aagcactggc caaggcggtc caggttcacc aagacactct gcgcaccatg
     4801 tactttgctt gagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     4861 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4921 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4981 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5041 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5101 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5581 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5641 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5701 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5761 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5821 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5881 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5941 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6001 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6061 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6121 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6181 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6241 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6301 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6361 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6421 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6481 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6541 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6601 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6661 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6721 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6781 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6841 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6901 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6961 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7021 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7081 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8401 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9241 ttccccgaaa agtgccacct gacgtc
//
