LOCUS       sequence-426751-        6108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  integrase plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Glass DS, Bren A, Vaisbourd E, Mayo A, Alon U
  TITLE     A synthetic differentiation circuit in Escherichia coli for 
            suppressing mutant takeover.
  JOURNAL   Cell. 2024 Jan 31:S0092-8674(24)00061-8. doi: 
            10.1016/j.cell.2024.01.024.
  PUBMED    38320549
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Jan 31:S0092-8674(24)00061-8. doi: 10.1016/j.cell.2024.01.024."
COMMENT     Sequence Label: pDSG578
FEATURES             Location/Qualifiers
     source          1..6108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..72)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2504..2590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    2604..2624
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2660..2691
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      complement(2794..2888)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2900..3691)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4049..4999)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5047..5269
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5761..5855)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(6011..6040)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    6087..6108
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
       61 tcgttttatt tgatgcctgg ggagtgtgag tgggaggtct gtatgtacgt cgagtacctt
      121 tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tccttaacgc
      181 tgtgtacccg gacaaacacc attaatcagc aggaaggtaa attcttcaat atcctgttca
      241 acggtcagct gttcggtcag taggcgatac caacaaaaac caaaaatcat atccagcagc
      301 agttcacgat tggtatcttt cggcagttca ccattgctaa tggcattttc aaccagtttt
      361 ttcggcatct cacgacgacg ttccataaac tgatctttca gctgggtcag ggttgcaggg
      421 tccagctgtg cttctgcaat aacacaacga aatgcttcac cacaaatggt ttcacgccaa
      481 actttccaca gattacgcag cagaaaatcc agatcggctt taaagctacc cagatccgga
      541 aatttacgca cctgttcgct ttcattttca tacacttcgg caatcagtgc tgctttattg
      601 gtccaccaac gataaatggt cggtttgctt gcaccggcac gacgtgcaac gctttcaatg
      661 ctcagaccgc tataaccaca ttctttcagg atttcaatgg tgctggtcag aattgcttta
      721 tgggtatgcg gactacgcag gctaccaatg ctgctacggc tcggggtacg tgccattaat
      781 tattcctact ttcagaggaa agttaaacaa aattatttgt agagggaaac cgttgtggtc
      841 tccctgaata tattatacga gccttatgca tgcccgtaaa gttatccagc aaccactcat
      901 agacctaggg cagcagatag ggacgacgtg gtgttagctg tgccgtcgga attgctatag
      961 cccttaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt tattgcgctc
     1021 tgattcgtta ccaattgaca tgatacgaaa cgtaccgtat cgttaaggtt acttagccat
     1081 cccgaaatac gtcgatataa gtaatgaagg cagctattta tatacgtgtt tctactcaag
     1141 agcaagtaga aaattattca atacaagctc aaactgaaaa actaacagga ttgtgccgct
     1201 cgaaggactg ggacgtatac gatattttca ttgacggcgg atactccggc tcaaatatga
     1261 atcgtcccgc attaaatgaa atgctaagta aactacacga aattgatgct gtagtcgtat
     1321 atcgattaga cagactatcc cgctcacaaa gagacacaat aacgcttatt gaagaatact
     1381 tcttaaaaaa caatgtagag tttgttagtt tgtctgaaac gcttgatacc agttcccctt
     1441 tcggtcgtgc aatgattggt atattatcag tattcgcaca gctagagcgc gaaacaatcc
     1501 gagatcgtat ggtgatgggg aaaattaagc gtattgaagc aggtcttccg ttaacaactg
     1561 cgaaaggtag aacgttcggc tatgatgtta tagatacaaa attatacatt aatgaagaag
     1621 aagcaaaaca gttacaactg atttatgata ttttcgaaga agaacaaagt ataacttttt
     1681 tacagaaaag actaaaaaaa ttaggcttta aagttagaac atataatcgc tataacaact
     1741 ggctaactaa tgatttgtat tgtggttatg tttcatataa agataaagtt catgtaaaag
     1801 gtattcatga acctatcatc agtgaagagc aattctatag agttcaagaa atatttactc
     1861 gtatgggtaa aaatccgaac atgaatagag attcagcatc gttgctaaat aatttagtag
     1921 tttgtagtaa atgcgggtta ggctttgttc atcgtagaaa agatacaatg tcgcgtggta
     1981 aaaaatatca ttatagatat tatagttgca agacttataa acatactcat gaactcgaaa
     2041 aatgcgggaa taaaatttgg agagctgaca aacttgaaga attaattatt aatcgtgtga
     2101 ataattatag tttcgcttcc agaaatgtag ataaagaaga tgaattagat agcttaaatg
     2161 aaaagcttaa aatagaacat gcaaagaaaa aacgattatt tgatttatat ataaatggct
     2221 cgtatgaagt ttcagaactt gattctatga tgaatgatat tgatgctcaa attaattatt
     2281 atgaatcaca aatagaagct aacgaagaat tgaagaaaaa caaaaagata caagaaaatt
     2341 tagctgattt agcaacagtt gattttgact ctttagagtt cagagaaaag caactttatt
     2401 taaaatcact aataaacaaa atttatattg atggtgaaca agttactatt gaatggctct
     2461 agtaataacc cgggggttcc catggtacgc gtgctagagg catcaaataa aacgaaaggc
     2521 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     2581 taggacaaat ccgccgccct agatactagt agcggccgct gcaggagtca ctaagggtta
     2641 gttagttaga ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc
     2701 cttggggtta tcattggggc tcactcaaag gcggtaatca gataaaaaaa atccttagct
     2761 ttcgctaagg atgatttctg ctagtatgct tggattctca ccaataaaaa acgcccggcg
     2821 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     2881 caggagtcca agcgagctct tatttgccga ctaccttggt gatctcgcct ttcacgtagt
     2941 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     3001 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     3061 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     3121 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     3181 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     3241 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     3301 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     3361 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     3421 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     3481 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     3541 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     3601 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     3661 tcgatacttc ggcgatcacc gcttccctca tacgaaacga tcctcatcct gtctcttgat
     3721 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     3781 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctc
     3841 tctagtagtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac caaaaactcg
     3901 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt
     3961 tccctttgat atgtaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc
     4021 actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct
     4081 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatacttac
     4141 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
     4201 catcgtgtag tgtttttctt agtccgttat gtaggtagga atctgatgta atggttgttg
     4261 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
     4321 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
     4381 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt
     4441 taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa
     4501 ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact
     4561 cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta
     4621 tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt
     4681 tttcgcttga gaacttggca tagtttgtcc actggaaaat cccaaagcct ttaaccaaag
     4741 gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat
     4801 aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg
     4861 tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa
     4921 ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga
     4981 aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat
     5041 tgagatgggc tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta
     5101 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
     5161 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
     5221 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
     5281 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
     5341 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
     5401 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     5461 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
     5521 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
     5581 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
     5641 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
     5701 acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg
     5761 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     5821 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     5881 acagctctag ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa
     5941 aaccgaaaag attacttcgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat
     6001 ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta
     6061 attagttagt tagcccttag tgactcgaat tcgcggccgc ttctagag
//
