LOCUS       sequence-426758-       12416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT 
            signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Dinsmore CJ, Soriano P
  TITLE     Conditional fluorescent mouse translocation reporters for ERK1/2 and
            AKT signaling.
  JOURNAL   Dev Biol. 2023 Sep 1:S0012-1606(23)00146-X. doi: 
            10.1016/j.ydbio.2023.08.007.
  PUBMED    37660778
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2023 Sep 1:S0012-1606(23)00146-X. doi: 10.1016/j.ydbio.2023.08.007."
COMMENT     Sequence Label: pROSA26-SAT
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..82
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    167..4507
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     polyA_signal    4608..4719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4708..4725
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     4727..4743
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4746..5334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4852..4863
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5261..5281
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(5352..5373)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5960..5981
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(6279..6335)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6351..6377)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6381..6407)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6411..6437)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7145..7154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7167..7544)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             complement(7545..7601)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7611..8318)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     regulatory      10277..10286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(10317..10350)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    10379..10513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gap             10760..10859
                     /estimated_length=100
     polyA_signal    11010..11471
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(11481..12284)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11592..11611)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12202..12221
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(12346..12365)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca
       61 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
      121 agaattcgat atcaagctta tcgataccgt cgacggtatc gataagctag atgtcctgaa
      181 atatttctat cagaacaagg tagtataaag ctggtaggta tacaaaacgc tagactagtt
      241 tctatccctg acccttaatc tgctagtata tccgtaggaa gttgcttaag tgccactagt
      301 accaacagcc tctgttagag tgaacagtag attttaatga agggccaata aagaccactt
      361 aagactactg actgaattaa aaatccaccc agtggagtta gtattggtta tagttatata
      421 acaaaatatc caacttagcc aggcgtggtg gtgcacgcct ttaatcccgg cactcaggag
      481 gcagaggcag gcggatttct gagttcgagg ccagcctggt ctacaaagtg agttccagga
      541 cagccagggc tatacagaga aaccctgtct cgaaaaacca aaaaaaaaaa aaaaaaggaa
      601 aaaaaatatg gcttgaaaac atgctattgt tgtcaaaagg agatccaaac aagtatttct
      661 ttgaacaaaa attatgtaga aaacagggtg ataaagaaca agggcttttt aaaaaaggcc
      721 agcattattt gagatataca agtgactcca attcaagcct aaaggccact caatgctcac
      781 taacagtgct aatcagaatc aattccttag cccagtcaaa aatagtcctg aatatcttct
      841 ggataaacat gtcctccaac atataaacta gtatacacta acaaaacgtc tcaacttcaa
      901 ggtgaaatgc ttgactccta gacttgtgac ccagcaaagt gctctatagg tagggttact
      961 aggtcagaga gtcttgcctg caaaccacaa ttatacaatt ccaccaaatg taagacatgt
     1021 aaaaatgact tatttagctc aggttttgaa aattaaaaat caaacccaca aagacctaac
     1081 tttcacatta agtacaaatg tttaacatat ataacatgtt ttgccttgat atatgaaatc
     1141 atatgacatc acctgacagg aaacttaagt ttatttgaac aacaatcagc ctaaggtagg
     1201 cctagcacat gatcaggata gtgcagggaa acccaaagaa gtgcttctga gtataaacca
     1261 agagacctcg aaatagcagc tttgttctgt atctcatggg ctttaactat cacttgaaaa
     1321 caatttcaca aatcacaaaa ccaacatttg tttagtttcc aactctgcct attcaccgga
     1381 gaaatccatg tcccaatttg atgggggaaa acttgaatga aatattattt ggaatatttt
     1441 aatatccctg tgtatatgca gatggtttaa agacaggtaa ccttaactgt agtttcttgg
     1501 ttgtctgcct tgttttttga aatatgctct caccaggagc ctgccaagta actactcttg
     1561 tgtgctcagc aagtcctagg gatcccttaa gcatgctcta acaggcctgg cttttttttt
     1621 taaaaagtag gttgcggtga taataattca agttttcatc acttgaaaca cattttacca
     1681 actatcaccc aagctccctt agtgtgtccc ctataaaaga atctgacctg caagttccaa
     1741 aagtattact acattttaac cttagtattc aggagacaaa agcaggcaga actttagtct
     1801 acatatcttg ttgcagatca ggcagagata catagtgaga ccctgcctca ggaaaagcta
     1861 tcatttttat attcacagta agcaaattaa cattaaaagt cagaaattct taatttgact
     1921 ttggctgtga agaatttgga ttctctggat ttgaaactag aaaatctgat taagcgatgc
     1981 aacagtttct gtatactaaa actctaagac ccttggttct aaagatacca catttaaaaa
     2041 aaaagttaag tataagacaa aaagccttaa tagctctttt ataggggagg gactcattta
     2101 atattagtcc acctcactcc tcataactat tttatgaggt ttattatata acccattttt
     2161 taaaaaaaaa attaaaggac aattttagtg tttgaaagat ttcccaaccc caccctggaa
     2221 atcaggctgc aaatctcagc agctgccctt agaaggcaaa aagccatgcc atttaagcca
     2281 tgggaagtta gtagcaaaca agagaccaca tggcaggaag gaggctttaa agaaagccca
     2341 cagtgtcaaa agacccaacc aacagcagag acagaaaaaa taatacccaa atctcatcag
     2401 aaaggtagac ggatttagcc acatccatag tggctcatta gggaatgctt caattataat
     2461 tttatgaaat ctcagagccc ccctccccaa cagggtttct gtgtgtattc ctggctatcc
     2521 tagatagaac ttgatgtgta gaccaggctg ggctcaaact cagagatctg cctgtctctg
     2581 cctccagagt gctgggatta aaggcataaa ggcaagcacc accactggct ggctaaactc
     2641 tggccctaca tttttgaaat ccaaaattat cattacttgc tttcatgttt agtaatggct
     2701 gcagacttag ctttcagctt tgtatagatg aagcacaaaa gttaagtaaa gctagcagtt
     2761 taaacaaata ggatactaga agttcaattt ttatttcttt attgcttagt gtaagagaaa
     2821 agacattaaa gtctgcattg gtcttctgta tcccacaagt ctgcagttat ggctcctctg
     2881 tccacagtta cacttcaaat aaaaattagt tccttggtaa catttccagt gcttaaagag
     2941 taatttgatt attggctacc atattggaac aaacacaaag tatttcatta cttcagtaaa
     3001 ttctactgga gacagtattc tctttaaaaa aaaaaaaaag aagaagaagg aggatttatt
     3061 tatttaatgt gaatacactt gtggtcttca gacacaccag aagagggcat cagatcccat
     3121 tacagatggt tgtgagccac catgtggttg ctgggaattg aactcaggac ttctggaaga
     3181 gcagtctgtg ctcttgaact ccgagccacc tctccagccc ctggatagta ttattataga
     3241 gcacaagcac acacaacaca acaacctaaa aaccaagctt cacaaataca tttgttgctt
     3301 aatcatttta cttgtgtttg gctacaagtc aagcaaaatt ataggtcctg aagaagcttg
     3361 gcaaaatcac atttagacca gcaataacgt gtagaatgcc atgagtcaag ccagtccaag
     3421 agaaagcact gcttaaatag aaaaaataca agctcacaag accttaggtc aggaaagaca
     3481 acaaacacct gaactttgca ttccaaaagg aaccaccttt tacagatgtg tacaatgaca
     3541 aaattttagt aagcagtaat caataccatg tggctcaata atgaaatata ccttttaatg
     3601 tcttaaaata tcagcctggc aatatgtaag atacatcagg taatattggg ggaggagaca
     3661 tccacctgga aaccattaat ggttaatagc tcagtttata aatggagaaa aaggagagag
     3721 gcattcatgg gagtggaaag ttaagcttta ggttggattc tcaatacatc tattgttgta
     3781 aaactccctg gactgagaat aggcccaaat gtggaacacc acctgacggg agaggtgata
     3841 gacactgaaa attaaggatc aaggcaaagg atcaacaaaa agtgtactaa ggagttataa
     3901 aagaactgca gtgttgaggc cccagctaca gcctcgattt gtggtgtatg taactaatct
     3961 gtctggtttc atgagtcatc agacttctaa gatcaggaaa gggaaaatgc caatgctctg
     4021 tctaggggtt ggataagcca gtataataaa tgaaaatggg gctaaaatga gtgttctaaa
     4081 ataccttttg ataaggctgc agaaggagcg ggagaaatgg atatgaagta ctgggctctt
     4141 taaaaatgat taaaattctg cttacatagt ctaactcgcg acactgtaat ttcatactgt
     4201 agtaaggatc tcaagcagga gagtataaaa ctcgggtgag catgtcttta atctacctcg
     4261 atggaaaata ctccgaggcg gatcacaagc aataataacc tgtagttttg ctgcataaaa
     4321 ccccagatga ctacctatcc tcccattttc cttatttgcc cctattaaaa aacttcccga
     4381 caaaaccgaa aatctgtggg aagtcttgtc cctccaattt tacacctgtt caattcccct
     4441 gcaggacaac gcccacacac caggttagcc tttaagcctg cccagaagac tcccgcccat
     4501 cttctagaaa gcttaagttt aaacgctagc tgtcttccca atcctccccc ttgctgtcct
     4561 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
     4621 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
     4681 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggtcgag gtcgacggta
     4741 tcgatgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
     4801 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
     4861 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
     4921 ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
     4981 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
     5041 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
     5101 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
     5161 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
     5221 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
     5281 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcgac
     5341 taggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     5401 ctcagcttgg actgatggct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     5461 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc aacgttgtgg
     5521 cggatcttga agttagcctt gatgcagttc ttctgcttgt cggccgtgat atagacgtag
     5581 tggctgttga agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     5641 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     5701 gtcttgtagg taccgtcgtc cttgaaagag atggtgcgct cctggacgta gccttcgggc
     5761 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcaggcc
     5821 acgccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     5881 cagatgaact tcagggtcag cttgccgttg gtggcatcgc cctcgccctc gccgcggacg
     5941 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     6001 aacagctcct cgcccttgct caccatacta gtggatggga attgaaagct ggactggagg
     6061 gtcaggcgct ccagccttga cgacaccgat ggggtacgtg gcttcttcga tggcgtcgct
     6121 gggtcctcga gaccgctacg cttgctgggc gaaagcgctg gccccggtgc ctgaagacca
     6181 gagcttgttc ctgatcctgg agtccgttca ggtccctggc cacccagcag gcttgggcta
     6241 agtggaagtt ccaggtcccg aggctttcgg cccttcatag gtccagggtt ctcctccacg
     6301 tctccagcct gcttcagcag gctgaagtta gtagctccgc ttcctccgcc agcgtaatct
     6361 ggaacgtcat atggatatcc tgcatagtcc gggacgtcat agggatagcc cgcatagtca
     6421 ggaacatcgt atgggtagtt gagcttatga cccaacttac taggcaaatc acagtagcgg
     6481 gcgacggcga cttcgtgctg ctcgacgtag gtttcgttgt tagcttcctt aatgcgctcc
     6541 agcctatagt ccacgtagta cacaccgggc atcttcaggt tcttcgctgg cttcttacta
     6601 cggtacgtag tcttggcatt ggctataagg tgcgaaccgc ccaccagctt cagagccata
     6661 tcgttgcgac cttcaagacc gccatcggcg gggtacagcg tctccgtaaa ggcctcccat
     6721 ccgagcgtct ttttctgcat aaccgggccg ttcgaggtaa agttcacgcc gcgtatcttc
     6781 acgttgtaaa tcaagcaacc atcctgcagg ctagtatctt gggtggcagt cagaactccg
     6841 ccgtcctcat aggtagtaac gcgttcccat gtgaagcctt cagggaagga ttgcttgaag
     6901 aaatctggta ttccctgagt atgattgatg aatgtcttgc tgccatagag gaaactggtg
     6961 gccaggatat cgaatgcaaa tggcagtgga cctccctcga caactttaat acgcatggtc
     7021 tgtgtgccct cgtagggctt tccctcaccc tcgctggtgc acttaaagtg gtggttgtcg
     7081 acggttccct ccatatacag cttcatgtgc atattctctt tgatcagctc ctctcccttc
     7141 gaaaccatgg tggcgaccgg tggatcctta gcgctggtgt acttggtgat ggccttagta
     7201 ccctcggaca cggcgtgctt ggccaactcc ccaggcagca gcaggcgcac ggccgtctgg
     7261 atctccctgg aggtgatggt cgagcgcttg ttgtaatgcg ccaggcggga agcctcacct
     7321 gcgatgcgct cgaaaatgtc gttcacaaac gaattcatga tgcccatggc cttggacgaa
     7381 atgccggtgt cagggtggac ctgcttcaga accttgtaca catagatgga atagctctcc
     7441 ttgcggctgc gcttgcgctt cttgccgcct ttcttctgcg ccttagtcac cgccttcttg
     7501 gagccctttt tcggggcggg agcagacttc gctggctctg gcataggtcc agggttctcc
     7561 tccacgtctc cagcctgctt cagcaggctg aagttagtag ctccgcttcc cttgtacagc
     7621 tcgtccatgc caccaccaag gttgctgtat ttggcaactg ccacttctct ttgcactacg
     7681 taggtttcat tgtcactctc ctcgatcctt tccaggcggt gatcaacggc atggacaccg
     7741 ggcatcttga tgttcccgac ggtctttttt gacctgtaag ttgtcacgaa gttgcagtgc
     7801 agatggccac caccatcaac tttcagtgcg atgtcagtgt atcctctcag accaccatct
     7861 gctggataca tcatctctgt attgggctcc caacccttgg tcttcttctg catcacggga
     7921 ccattggagg gaaagtttac ccctctgacc ttgacgttgt agacgagctc gccatcctca
     7981 aggctggtgt cctgcgtgac ggtgacgact ccaccatctt cgtatctcgt aactctttcc
     8041 caagtaaaac cctcaggaaa ggactgttta aagaaatcag ggatgtcggc cgggtacttg
     8101 ataaaggtac ggctgccata catgaacgac gtggcaagaa tgtcaaaggc aaatggcagg
     8161 ggtcctccct cgatgacttt gatcctcatg gtttgcactc cctcgtacgg tctgccttct
     8221 ccttcacctg tgcatttgaa ttggtggccg ttgaccgaac cttccatgac caccttcata
     8281 cgcatatttt ccttgatcag ctcttcgccc ttagacacag atcctcctga cacccagctg
     8341 tgtgttgtag tcttgacact gtgtgggaag ctttggttgg gcaacacatt atcaaagtta
     8401 aaatccaagg tatctccatc catggggtca ttccgaatga tggactccat gtcacagtcc
     8461 aagcgctcaa taaacatgcc atccaagtca cttgggagct tctcctggtg gaggacaccc
     8521 atcctaccat agccattgca gctgctcacc gaggagtagt tgcccagggc actcatctgc
     8581 atggggtggg acagaggcac ttgtaaaggt gtcttcacgg gggtcaagcg gttcatggca
     8641 gatgtgtgag gcatggtgtt caccacatgg ggcagggtac ggccgttgac cgaagccgtt
     8701 tgctgtgcat gtccagggtg ggtgtgggag ctggggtaca tcattttgtt atgagatgcc
     8761 tggctgccat acgctggcat gaccgaatta gggcccatca ttacattttg gcccaggacc
     8821 cgactgttgg gttgggccac tccgggatca accggtgaca taatgtcatt gtggggagga
     8881 gagtcagaag tcaacaactc tttcaagagt cctggggcac agttatactg gttcaatcct
     8941 ccgtaacttg atttgctgtc ctgaagtgtc tgcataggca tctggggcaa agggctcatg
     9001 ctggattggc cgtatgtgta ctttgagtag tttggactgg gtgaatttag actggtgttt
     9061 ggcggtgcaa acgaatagca tggtgtctgc tgcatcatgc tgccaggcga ggactgagtg
     9121 gacacagtta aagatgttgg ggacgagaga aggttgagat tatccagaag gttctccatg
     9181 ttttctggat tgctgatttc agacagactg ggcagcgtag acgccatctt ggcagcagag
     9241 ggtggataca ccagggaatg cacgtcccca tctcccaggt catcctgctc tgtcatgatg
     9301 ggagaaagtc tcccactgat ggtactagca tttgagctgg ttcgaggacg aaatgtactc
     9361 cagttatcaa agtcatcgtt gctgtgggac ccaggactcg caggccactt agaaaactga
     9421 gacccagggc tgtctccagg accctcttgc ccagaccgga gagatgcttt tttcttagca
     9481 gcccgccctc ggctcttagc aaatttactg ttgttgtcca tggacgcagc tcttctccgg
     9541 ggtgattttc cgctcttgcc tccctctgga ttgagcatcc accaagaact ctttccagtt
     9601 ccttcattct gcactcgaat aaacttgctg tgaagggcta gattgcggcg aattgcattc
     9661 ttccagcccg ccgagctgtt gctgtcgccc ttatccttga agtagggcac gctcttcacc
     9721 atccactcgt agatctgcga cagggtgagc ctcttctcgg ctgagctctc gatggccttg
     9781 gtgatgaggt cggcgtacga caggttgccc cacgcgttgc ggcgcgacga gctggtcttg
     9841 cgcggctgtc ccgcgagtgg ccccgcggcg gcggcagcgg agggaggcac gggtgggggc
     9901 tgcgacagcg gcccggtcgg tgggggctgt ggcggcgctg ggtgcacgca gcccgcctcg
     9961 gggccctgga agtccccgca caggcccctg ctggccgcag ccgccgccgc cacggccacg
    10021 cagcctggcg cccgcgcgaa gtcctcactc tcctccagca ggctcaggtt gctcataaag
    10081 tcggtgctga cagcggacgc cgaggccagg ctcgccgcgg cgtcggggtt ggcggccgcg
    10141 ccgcccgacg gcgccggact ggaggtggtc gagttggact ggttaaactc cggcctgggc
    10201 agcggccagg tacaggagcg ctgccggggc agcggctcga agtccgggtc ggtctccacc
    10261 acctggggag cctctgccat ggtggcgcta gcttgggctg caggtcgagg gacctaataa
    10321 cttcgtatag catacattat acgaagttat attaagggtt ccggatcagc ttgatgggga
    10381 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
    10441 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
    10501 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
    10561 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
    10621 tctagagtcg cagatccaga catgataaga tacattgatg agtttggaca aaccacaact
    10681 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
    10741 accattataa gctgcaatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    10861 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
    10921 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagagtcgc
    10981 agatcctcta gagtcgcaga tctgcaagct tctgatggaa ttagaacttg gcaaaacaat
    11041 actgagaatg aagtgtatgt ggaacagagg ctgctgatct cgttcttcag gctatgaaac
    11101 tgacacattt ggaaaccaca gtacttagaa ccacaaagtg ggaatcaaga gaaaaacaat
    11161 gatcccacga gagatctata gatctataga tcatgagtgg gaggaatgag ctggccctta
    11221 atttggtttt gcttgtttaa attatgatat ccaactatga aacattatca taaagcaata
    11281 gtaaagagcc ttcagtaaag agcaggcatt tatctaatcc caccccaccc ccacccccgt
    11341 agctccaatc cttccattca aaatgtaggt actctgttct cacccttctt aacaaagtat
    11401 gacaggaaaa acttccattt tagtggacat ctttattgtt taatagatca tcaatttctg
    11461 cagacttaca gcggatcccc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
    11521 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
    11581 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
    11641 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
    11701 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg
    11761 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
    11821 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
    11881 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
    11941 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
    12001 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
    12061 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
    12121 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
    12181 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
    12241 ggagaacctg cgtgcaatcc atcttgttca atggccgatc ccatattggc tgcaggtcga
    12301 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
    12361 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccc
//
