LOCUS       sequence-426766-        8319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-seq crRNA expression vector with GFP and neo marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, 
            Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA
  TITLE     Engineered CRISPR-Cas12a for higher-order combinatorial chromatin 
            perturbations.
  JOURNAL   Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0.
  PUBMED    38760567
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0."
COMMENT     Sequence Label: pCH49 (crRNA expression vector)
FEATURES             Location/Qualifiers
     source          1..8319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             417..597
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    675..796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(712..731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     766..785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      836..971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(992..1010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1020..1037)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1020..1036)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1029..1051)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1178..1633
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1265..1284)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1475..1496
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1659..1763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1764..1832,1833..2624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1982..2001)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2795..3383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3284..3303
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3537..3554
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3671..3692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3707..3712,3713..3730,3731..3737)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3745..3761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3750..3772
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3769..3785
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3769..3785
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3804..3824
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3806..3824
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3852..4078
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4079..4259
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4306..4431
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4924..5157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5342..5386
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5567..5684
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5768..5979
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5999..6008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6008..6031
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6032..6034,6035..6037,6038..6748)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6032..6034,6035..6037,6038..6748)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6077..6098)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6338..6357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6685..6706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6758..6814
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6815..7609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6869..6888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7479..7498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7625..8213
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7678..7698)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8096..8107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagt ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
      301 atttctactc ttgtagatcg agacggttgt atttgtacgt ctcaaatttc tcctctagga
      361 gatttttttt aagcttggcg taactagatc ttgagacact gctttttgct tgtactgggt
      421 ctctcttgtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      481 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      541 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
      601 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
      661 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      781 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
      841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
      901 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
      961 ttttggaggc ctagggacgt acccaattcg tcctatagtg agtcgtatta cgcgcgctca
     1021 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1081 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1141 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1201 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1261 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1321 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1381 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1441 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1501 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1561 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1621 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1681 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1741 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1801 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1861 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1921 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1981 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2041 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2101 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2161 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2221 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2281 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2341 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2401 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2461 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2521 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2581 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2641 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2701 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2761 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2821 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2881 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2941 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3001 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3061 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3121 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3181 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3241 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3301 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3361 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3421 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3481 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3541 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3601 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3661 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     3721 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     3781 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     3841 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     3901 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     3961 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     4021 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     4081 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4141 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4201 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4261 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4321 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4381 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4441 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4501 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4561 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     4621 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     4681 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     4741 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     4801 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     4861 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     4921 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     4981 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5041 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5101 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5161 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5221 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5281 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5341 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5401 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5461 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5521 attatcgttt cagaatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     5581 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     5641 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     5701 acagcagaga tccactttgg cgccggctcg agggggcccg ggtcgagtgg ctccggtgcc
     5761 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5821 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5881 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5941 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg cgggatccgc
     6001 caccatggat tacaaagacg atgacgataa gatggtgagc aagggcgagg agctgttcac
     6061 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     6121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     6181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     6241 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     6301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     6361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     6421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6481 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     6541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6721 cactctcggc atggacgagc tgtacaaggg cagcggcgcc accaacttca gcctgctgaa
     6781 gcaggccggc gacgtggagg agaaccccgg ccccatgctt gaacaagatg gattgcacgc
     6841 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6901 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6961 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     7021 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7081 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7141 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7201 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7261 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7321 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7381 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7441 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7501 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7561 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa cgcgttaagt
     7621 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7681 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7741 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7801 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7861 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7921 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7981 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8041 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8101 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8161 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     8221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8281 ggactggaag ggctaattca ctcccaacga agacaagat
//
