LOCUS       sequence-426776-       11010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AtAFB2.F74A mutant with mCherry tag for AID2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Li S, Wang Y, van der Stoel M, Zhou X, Madhusudan S, Kanerva K, 
            Nguyen VD, Eskici N, Olkkonen VM, Zhou Y, Raivio T, Ikonen E
  TITLE     HiHo-AID2: boosting homozygous knock-in efficiency enables robust 
            generation of human auxin-inducible degron cells.
  JOURNAL   Genome Biol. 2024 Feb 26;25(1):58. doi: 10.1186/s13059-024-03187-w.
  PUBMED    38409044
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2024-02-26"; volume: "25(1):58. doi"; pages: " 
            10.1186/s13059-024-03187-w"
COMMENT     Sequence Label: pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro
FEATURES             Location/Qualifiers
     source          1..11010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(135..152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(306..894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(386..405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1065..1856,1857..1925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1688..1707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1926..2030)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2057..2512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2144..2163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2354..2375
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    3029..3832
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    3858..3906
                     /note="synthetic polyadenylation signal"
     misc_feature    3920..4011
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3960..3979
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        4036..4415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4417..4693
                     /label=chicken beta-actin promoter
     intron          4694..5710
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5634..5656
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5718..5737
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7525..8232
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7667..7685)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7935..7954
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8251..8817
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8267..8819
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8411..8428)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8638..8657
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8819..9421
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9318..9338
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9655..9736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9692..9711)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    9764..10600
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
       61 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
      121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2041 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg
     2521 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2581 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     2641 gtcgacctac tctcttccgc attggagtcg ctttaactgg ccctggcttt ggcagcctgt
     2701 gctgacccat gcagtcctcc ttaccatccc tccctcgact tcccctcttc cgatgttgag
     2761 cccctccagc cggtcctgga ctttgtctcc ttccctgccc tgccctctcc tgaacctgag
     2821 ccagctccca tagctcagtc tggtctatct gcctggccct ggccattgtc actttgcgct
     2881 gccctcctct cgcccccgag tgcccttgct gtgccgccgg aactctgccc tctaacgctg
     2941 ccgtctctct cctgagtccg gaccactttg agctctactg gcttctgcgc cgcctctggc
     3001 ccactgtttc cccttcccag gcaggtcctg ctttctctga cctgcattct ctcccctggg
     3061 cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct ggcccagatc
     3121 cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc ttgctctctg
     3181 ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc tgcaccacgt
     3241 gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct ctcctccctc
     3301 acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt ctggggcctg
     3361 tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg cgtcccgcct
     3421 ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag taccccgtct
     3481 ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc ccgttctcct
     3541 gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc cccttacctc
     3601 tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg tccgagagct
     3661 cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag catgtttgct
     3721 gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg gccggttaat
     3781 gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg gcgcatgcgt
     3841 ggaattatca cctcgagaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     3901 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     3961 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tactagtggg
     4021 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4081 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4141 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4201 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4261 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4321 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4381 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
     4441 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
     4501 tattttgtgc agcgatgggg gcgggggggg gggggggcgc gcgccaggcg gggcggggcg
     4561 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
     4621 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     4681 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
     4741 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     4801 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     4861 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     4921 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     4981 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     5041 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     5101 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     5161 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     5221 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     5281 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     5341 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     5401 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     5461 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     5521 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     5581 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     5641 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     5701 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     5761 attgctaccg gactcagatc taccatgaac tactttcccg atgaggtcat tgagcacgtc
     5821 tttgatttcg tcacaagcca caaggatagg aacgccatta gcctggtctg taagagctgg
     5881 tacaagatcg agcggtattc cagacagaag gtgttcatcg gcaactgcta cgccatcaat
     5941 ccagagaggc tgctgcggag atttccctgt ctgaagagcc tgaccctgaa gggcaagccc
     6001 cacgccgccg actttaacct ggtgcctcac gagtggggag gattcgtgct gccatggatc
     6061 gaggccctgg cacggtccag agtgggcctg gaggagctga ggctgaagcg catggtggtg
     6121 acagacgagt ctctggagct gctgtctcgg agcttcgtga acttcaagag cctggtgctg
     6181 gtgtcctgcg agggctttac cacagatggc ctggcaagca tcgcagcaaa ctgtaggcac
     6241 ctgcgggacc tggacctgca ggagaatgag atcgacgatc acagaggcca gtggctgtcc
     6301 tgcttccccg atacctgtac cacactggtg acactgaact ttgcctgcct ggagggcgag
     6361 acaaatctgg tggccctgga gaggctggtg gcacgctctc ccaacctgaa gagcctgaag
     6421 ctgaataggg cagtgcctct ggacgcactg gcaagactga tggcatgcgc accacagatc
     6481 gtggatctgg gcgtgggctc ctacgagaac gaccctgatt ccgagtctta tctgaagctg
     6541 atggccgtga tcaagaagtg taccagcctg cgcagcctgt ccggcttcct ggaggcagca
     6601 cctcactgcc tgtccgcctt tcacccaatc tgtcacaacc tgacatccct gaatctgtct
     6661 tacgccgccg agatccacgg ctcccacctg atcaagctga tccagcactg caagaagctg
     6721 cagaggctgt ggatcctgga ctccatcggc gataagggcc tggaggtggt ggcctctacc
     6781 tgtaaggagc tgcaggagct gcgcgtgttc ccatctgacc tgctgggagg aggaaacacc
     6841 gcagtgacag aggagggcct ggtggcaatc tctgccggat gccccaagct gcacagcatc
     6901 ctgtattttt gtcagcagat gaccaacgcc gccctggtga cagtggccaa gaactgcccc
     6961 aatttcatca ggtttcgcct gtgcatcctg gagcccaata agcctgacca cgtgacatct
     7021 cagcctctgg atgagggctt cggcgccatc gtgaaggcct gcaagagcct gaggcgcctg
     7081 tctctgagcg gcctgctgac cgaccaggtg ttcctgtaca tcggcatgta tgccaaccag
     7141 ctggagatgc tgtctatcgc ctttgccggc gacacagata agggcatgct gtacgtgctg
     7201 aatggctgta agaagatgaa gaagctggag atcagagaca gcccttttgg cgataccgcc
     7261 ctgctggcag acgtgagcaa gtatgagaca atgcggagcc tgtggatgag ctcctgcgag
     7321 gtgaccctga gcggctgtaa gagactggcc gagaaggccc catggctgaa cgtggagatc
     7381 atcaacgaga atgacaacaa taggatggag gagaatggcc acgagggccg ccagaaggtg
     7441 gataagctgt atctgtatag gaccgtcgtc gggactcgga tggatgcccc cccctttgtc
     7501 tggattctgg gcggatctgg cggggtgagc aagggcgagg aggataacat ggccatcatc
     7561 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     7621 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     7681 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     7741 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     7801 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     7861 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     7921 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     7981 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     8041 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     8101 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     8161 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     8221 ctgtacaagt agcggccgct ctagcccaat tccgcccccc cccctaacgt tactggccga
     8281 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     8341 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     8401 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     8461 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     8521 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     8581 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     8641 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     8701 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     8761 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     8821 gggcaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     8881 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     8941 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9001 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     9061 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9121 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9181 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9241 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9301 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9361 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     9421 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atggctccga
     9481 ccgaagccac ccggggcggc cccgccgacc ccgcacccgc ccccgaggcc caccgactct
     9541 agctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9601 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9661 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9721 gcattttttt cactgcattc tagttgtggt ttgtccatgc atcactaggg acaggattgg
     9781 tgacagaaaa gccccatcct taggcctcct ccttcctagt ctcctgatat tgggtctaac
     9841 ccccacctcc tgttaggcag attccttatc tggtgacaca cccccatttc ctggagccat
     9901 ctctctcctt gccagaacct ctaaggtttg cttacgatgg agccagagag gatcctggga
     9961 gggagagctt ggcagggggt gggagggaag ggggggatgc gtgacctgcc cggttctcag
    10021 tggccaccct gcgctaccct ctcccagaac ctgagctgct ctgacgcggc tgtctggtgc
    10081 gtttcactga tcctggtgct gcagcttcct tacacttccc aagaggagaa gcagtttgga
    10141 aaaacaaaat cagaataagt tggtcctgag ttctaacttt ggctcttcac ctttctagtc
    10201 cccaatttat attgttcctc cgtgcgtcag ttttacctgt gagataaggc cagtagccag
    10261 ccccgtcctg gcagggctgt ggtgaggagg ggggtgtccg tgtggaaaac tccctttgtg
    10321 agaatggtgc gtcctaggtg ttcaccaggt cgtggccgcc tctactccct ttctctttct
    10381 ccatccttct ttccttaaag agtccccagt gctatctggg acatattcct ccgcccagag
    10441 cagggtcccg cttccctaag gccctgctct gggcttctgg gtttgagtcc ttggcaagcc
    10501 caggagaggc gctcaggctt ccctgtcccc cttcctcgtc caccatctca tgcccctggc
    10561 tctcctgccc cttccctaca ggggttcctg gctctgctct tcagactgag ccccgttccc
    10621 ctgcatcccc gttcccctgc atcccccttc ccctgcatcc cccagaggcc ccaggccacc
    10681 tacttggcct ggaccccacg agaggccacc ccagccctgt ctaccaggct gccttttggg
    10741 tggattctcc tccaactgtg gggtgactgc ttggcaaact cactcttcgg ggtatcccag
    10801 gaggcctgga gcattggggt gggctggggt tcagagagga gggattccct tctcaggtta
    10861 cgtggccaag aagcagggga gctgggtttg ggtcaggtct gggtgtgggg tgaccagctt
    10921 atgctgtttg cccaggacag cctagtttta gcgctgaaac cctcagtcct aggaaaacag
    10981 ggatggttgg tcactgtctc tgggctcgac
//
