LOCUS       sequence-426790-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: AcGFP-MSH3
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2910
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        6411..6910
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6446..6469)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6843..6862
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6931..7530
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6931..6950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7427..7447
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7715..8147
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(8250..8272)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8408..8428)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8413..8742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8593..8728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8764..8783
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8864..8881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9035..9623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9115..9134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9794..10585,10586..10654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10417..10436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10655..10759)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10827..10845
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10945..11016,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcgc cgagctgttc
     2221 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
     2281 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2341 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg
     2401 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
     2461 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg
     2521 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc
     2581 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac
     2641 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc
     2701 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
     2761 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
     2821 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc
     2881 atcacccacg gcatggatga gctgtacaag tccggactca gatctggagg cggaggaagc
     2941 tctcgccgga agcctgcgtc gggcggcctc gctgcctcca gctcagcccc tgcgaggcaa
     3001 gcggttttga gccgattctt ccagtctacg ggaagcctga aatccacctc ctcctccaca
     3061 ggtgcagccg accaggtgga ccctggcgct gcagcggctg cagcggccgc agcggccgca
     3121 gcgcccccag cgcccccagc tcccgccttc ccgccccagc tgccgccgca catagctaca
     3181 gaaattgaca gaagaaagaa gagaccattg gaaaatgatg ggcctgttaa aaagaaagta
     3241 aagaaagtcc aacaaaagga aggaggaagt gatctgggaa tgtctggcaa ctctgagcca
     3301 aagaaatgtc tgaggaccag gaatgtttca aagtctctgg aaaaattgaa agaattctgc
     3361 tgcgattctg cccttcctca aagtagagtc cagacagaat ctctgcagga gagatttgca
     3421 gttctgccaa aatgtactga ttttgatgat atcagtcttc tacacgcaaa gaatgcagtt
     3481 tcttctgaag attcgaaacg tcaaattaat caaaaggaca caacactttt tgatctcagt
     3541 cagtttggat catcaaatac aagtcatgaa aatttacaga aaactgcttc caaatcagct
     3601 aacaaacggt ccaaaagcat ctatacgccg ctagaattac aatacataga aatgaagcag
     3661 cagcacaaag atgcagtttt gtgtgtggaa tgtggatata agtatagatt ctttggggaa
     3721 gatgcagaga ttgcagcccg agagctcaat atttattgcc atttagatca caactttatg
     3781 acagcaagta tacctactca cagactgttt gttcatgtac gccgcctggt ggcaaaagga
     3841 tataaggtgg gagttgtgaa gcaaactgaa actgcagcat taaaggccat tggagacaac
     3901 agaagttcac tcttttcccg gaaattgact gccctttata caaaatctac acttattgga
     3961 gaagatgtga atcccctaat caagctggat gatgctgtaa atgttgatga gataatgact
     4021 gatacttcta ccagctatct tctgtgcatc tctgaaaata aggaaaatgt tagggacaaa
     4081 aaaaagggca acatttttat tggcattgtg ggagtgcagc ctgccacagg cgaggttgtg
     4141 tttgatagtt tccaggactc tgcttctcgt tcagagctag aaacccggat gtcaagcctg
     4201 cagccagtag agctgctgct tccttcggcc ttgtccgagc aaacagaggc gctcatccac
     4261 agagccacat ctgttagtgt gcaggatgac agaattcgag tcgaaaggat ggataacatt
     4321 tattttgaat acagccatgc tttccaggca gttacagagt tttatgcaaa agatacagtt
     4381 gacatcaaag gttctcaaat tatttctggc attgttaact tagagaagcc tgtgatttgc
     4441 tctttggctg ccatcataaa atacctcaaa gaattcaact tggaaaagat gctctccaaa
     4501 cctgagaatt ttaaacagct atcaagtaaa atggaattta tgacaattaa tggaacaaca
     4561 ttaaggaatc tggaaatcct acagaatcag actgatatga aaaccaaagg aagtttgctg
     4621 tgggttttag accacactaa aacttcattt gggagacgga agttaaagaa gtgggtgacc
     4681 cagccactcc ttaaattaag ggaaataaat gcccggcttg atgctgtatc ggaagttctc
     4741 cattcagaat ctagtgtgtt tggtcagata gaaaatcatc tacgtaaatt gcccgacata
     4801 gagaggggac tctgtagcat ttatcacaaa aaatgttcta cccaagagtt cttcttgatt
     4861 gtcaaaactt tatatcacct aaagtcagaa tttcaagcaa taatacctgc tgttaattcc
     4921 cacattcagt cagacttgct ccggaccgtt attttagaaa ttcctgaact cctcagtcca
     4981 gtggagcatt acttaaagat actcaatgaa caagctgcca aagttgggga taaaactgaa
     5041 ttatttaaag acctttctga cttcccttta ataaaaaaga ggaaggatga aattcaaggt
     5101 gttattgacg agatccgaat gcatttgcaa gaaatacgaa aaatactaaa aaatccttct
     5161 gcacaatatg tgacagtatc aggacaggag tttatgatag aaataaagaa ctctgctgta
     5221 tcttgtatac caactgattg ggtaaaggtt ggaagcacaa aagctgtgag ccgctttcac
     5281 tctcctttta ttgtagaaaa ttacagacat ctgaatcagc tccgggagca gctagtcctt
     5341 gactgcagtg ctgaatggct tgattttcta gagaaattca gtgaacatta tcactccttg
     5401 tgtaaagcag tgcatcacct agcaactgtt gactgcattt tctccctggc caaggtcgct
     5461 aagcaaggag attactgcag accaactgta caagaagaaa gaaaaattgt aataaaaaat
     5521 ggaaggcacc ctgtgattga tgtgttgctg ggagaacagg atcaatatgt cccaaataat
     5581 acagatttat cagaggactc agagagagta atgataatta ccggaccaaa catgggtgga
     5641 aagagctcct acataaaaca agttgcattg attaccatca tggctcagat tggctcctat
     5701 gttcctgcag aagaagcgac aattgggatt gtggatggca ttttcacaag gatgggtgct
     5761 gcagacaata tatataaagg acagagtaca tttatggaag aactgactga cacagcagaa
     5821 ataatcagaa aagcaacatc acagtccttg gttatcttgg atgaactagg aagagggacg
     5881 agcactcatg atggaattgc cattgcctat gctacacttg agtatttcat cagagatgtg
     5941 aaatccttaa ccctgtttgt cacccattat ccgccagttt gtgaactaga aaaaaattac
     6001 tcacaccagg tggggaatta ccacatggga ttcttggtca gtgaggatga aagcaaactg
     6061 gatccaggcg cagcagaaca agtccctgat tttgtcacct tcctttacca aataactaga
     6121 ggaattgcag caaggagtta tggattaaat gtggctaaac tagcagatgt tcctggagaa
     6181 attttgaaga aagcagctca caagtcaaaa gagctggaag gattaataaa tacgaaaaga
     6241 aagagactca agtattttgc aaagttatgg acgatgcata atgcacaaga cctgcagaag
     6301 tggacagagg agttcaacat ggaagaaaca cagacttctc ttcttcatta agtcgacggt
     6361 accgcgggcc cgggatccac cggatctaga taactgatca taattctacc gggtagggga
     6421 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     6481 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     6541 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     6601 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     6661 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     6721 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     6781 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     6841 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     6901 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     6961 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7021 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7081 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7141 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7201 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7261 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7321 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7381 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7441 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7501 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     7561 gaaaggagcg cacgacccca tgcatcggcg tctcgatcga gatatcagtg gtccaggctc
     7621 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat
     7681 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     7741 gcaagctact agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     7801 tagagaagtt cagatcaagg tcaggaacag atggaacagg gtcgatcgac cctagagaac
     7861 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     7921 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     7981 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     8041 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     8101 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     8161 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc
     8221 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8281 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8341 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc
     8401 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     8461 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8521 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8641 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8701 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact
     8761 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     8821 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     8881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9541 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9601 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9661 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9721 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10081 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
    10141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10381 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
    10441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10681 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10741 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10801 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
    10861 aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
    10921 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
    10981 ttacggggtc attagttcat agcccatata tggagt
//
