LOCUS       sequence-426791-       11667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AcGFP-MSH6 in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: AcGFP-MSH6
FEATURES             Location/Qualifiers
     source          1..11667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2910
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        7062..7561
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7097..7120)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7494..7513
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7582..8181
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7582..7601)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8078..8098
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8366..8798
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(8901..8923)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9059..9079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9064..9393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9244..9379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9415..9434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9515..9532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9686..10274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9766..9785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10445..11236,11237..11305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11068..11087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11306..11410)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11478..11496
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(11596..11667,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcgc cgagctgttc
     2221 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
     2281 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2341 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg
     2401 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
     2461 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg
     2521 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc
     2581 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac
     2641 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc
     2701 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
     2761 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
     2821 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc
     2881 atcacccacg gcatggatga gctgtacaag tccggactca gatctcgagg aggcggagga
     2941 agctcgcgac agagcaccct gtacagcttc ttccccaagt ctccggcgct gagtgatgcc
     3001 aacaaggcct cggccagggc ctcacgcgaa ggcggccgtg ccgccgctgc ccccggggcc
     3061 tctccttccc caggcgggga tgcggcctgg agcgaggctg ggcctgggcc caggcccttg
     3121 gcgcgctccg cgtcaccgcc caaggcgaag aacctcaacg gagggctgcg gagatcggta
     3181 gcgcctgctg cccccaccag ttgtgacttc tcaccaggag atttggtttg ggccaagatg
     3241 gagggttacc cctggtggcc ttgtctggtt tacaaccacc cctttgatgg aacattcatc
     3301 cgcgagaaag ggaaatcagt ccgtgttcat gtacagtttt ttgatgacag cccaacaagg
     3361 ggctgggtta gcaaaaggct tttaaagcca tatacaggtt caaaatcaaa ggaagcccag
     3421 aagggaggtc atttttacag tgcaaagcct gaaatactga gagcaatgca acgtgcagat
     3481 gaagccttaa ataaagacaa gattaagagg cttgaattgg cagtttgtga tgagccctca
     3541 gagccagaag aggaagaaga gatggaggta ggcacaactt acgtaacaga taagagtgaa
     3601 gaagataatg aaattgagag tgaagaggaa gtacagccta agacacaagg atctaggcga
     3661 agtagccgcc aaataaaaaa acgaagggtc atatcagatt ctgagagtga cattggtggc
     3721 tctgatgtgg aatttaagcc agacactaag gaggaaggaa gcagtgatga aataagcagt
     3781 ggagtggggg atagtgagag tgaaggcctg aacagccctg tcaaagttgc tcgaaagcgg
     3841 aagagaatgg tgactggaaa tggctctctt aaaaggaaaa gctctaggaa ggaaacgccc
     3901 tcagccacca aacaagcaac tagcatttca tcagaaacca agaatacttt gagagctttc
     3961 tctgcccctc aaaattctga atcccaagcc cacgttagtg gaggtggtga tgacagtagt
     4021 cgccctactg tttggtatca tgaaacttta gaatggctta aggaggaaaa gagaagagat
     4081 gagcacagga ggaggcctga tcaccccgat tttgatgcat ctacactcta tgtgcctgag
     4141 gatttcctca attcttgtac tcctgggatg aggaagtggt ggcagattaa gtctcagaac
     4201 tttgatcttg tcatctgtta caaggtgggg aaattttatg agctgtacca catggatgct
     4261 cttattggag tcagtgaact ggggctggta ttcatgaaag gcaactgggc ccattctggc
     4321 tttcctgaaa ttgcatttgg ccgttattca gattccctgg tgcagaaggg ctataaagta
     4381 gcacgagtgg aacagactga gactccagaa atgatggagg cacgatgtag aaagatggca
     4441 catatatcca agtatgatag agtggtgagg agggagatct gtaggatcat taccaagggt
     4501 acacagactt acagtgtgct ggaaggtgat ccctctgaga actacagtaa gtatcttctt
     4561 agcctcaaag aaaaagagga agattcttct ggccatactc gtgcatatgg tgtgtgcttt
     4621 gttgatactt cactgggaaa gtttttcata ggtcagtttt cagatgatcg ccattgttcg
     4681 agatttagga ctctagtggc acactatccc ccagtacaag ttttatttga aaaaggaaat
     4741 ctctcaaagg aaactaaaac aattctaaag agttcattgt cctgttctct tcaggaaggt
     4801 ctgatacccg gctcccagtt ttgggatgca tccaaaactt tgagaactct ccttgaggaa
     4861 gaatatttta gggaaaagct aagtgatggc attggggtga tgttacccca ggtgcttaaa
     4921 ggtatgactt cagagtctga ttccattggg ttgacaccag gagagaaaag tgaattggcc
     4981 ctctctgctc taggtggttg tgtcttctac ctcaaaaaat gccttattga tcaggagctt
     5041 ttatcaatgg ctaattttga agaatatatt cccttggatt ctgacacagt cagcactaca
     5101 agatctggtg ctatcttcac caaagcctat caacgaatgg tgctagatgc agtgacatta
     5161 aacaacttgg agatttttct gaatggaaca aatggttcta ctgaaggaac cctactagag
     5221 agggttgata cttgccatac tccttttggt aagcggctcc taaagcaatg gctttgtgcc
     5281 ccactctgta accattatgc tattaatgat cgtctagatg ccatagaaga cctcatggtt
     5341 gtgcctgaca aaatctccga agttgtagag cttctaaaga agcttccaga tcttgagagg
     5401 ctactcagta aaattcataa tgttgggtct cccctgaaga gtcagaacca cccagacagc
     5461 agggctataa tgtatgaaga aactacatac agcaagaaga agattattga ttttctttct
     5521 gctctggaag gattcaaagt aatgtgtaaa attataggga tcatggaaga agttgctgat
     5581 ggttttaagt ctaaaatcct taagcaggtc atctctctgc agacaaaaaa tcctgaaggt
     5641 cgttttcctg atttgactgt agaattgaac cgatgggata cagcctttga ccatgaaaag
     5701 gctcgaaaga ctggacttat tactcccaaa gcaggctttg actctgatta tgaccaagct
     5761 cttgctgaca taagagaaaa tgaacagagc ctcctggaat acctagagaa acagcgcaac
     5821 agaattggct gtaggaccat agtctattgg gggattggta ggaaccgtta ccagctggaa
     5881 attcctgaga atttcaccac tcgcaatttg ccagaagaat acgagttgaa atctaccaag
     5941 aagggctgta aacgatactg gaccaaaact attgaaaaga agttggctaa tctcataaat
     6001 gctgaagaac ggagggatgt atcattgaag gactgcatgc ggcgactgtt ctataacttt
     6061 gataaaaatt acaaggactg gcagtctgct gtagagtgta tcgcagtgtt ggatgtttta
     6121 ctgtgcctgg ctaactatag tcgagggggt gatggtccta tgtgtcgccc agtaattctg
     6181 ttgccggaag ataccccccc cttcttagag cttaaaggat cacgccatcc ttgcattacg
     6241 aagacttttt ttggagatga ttttattcct aatgacattc taataggctg tgaggaagag
     6301 gagcaggaaa atggcaaagc ctattgtgtg cttgttactg gaccaaatat ggggggcaag
     6361 tctacgctta tgagacaggc tggcttatta gctgtaatgg cccagatggg ttgttacgtc
     6421 cctgctgaag tgtgcaggct cacaccaatt gatagagtgt ttactagact tggtgcctca
     6481 gacagaataa tgtcaggtga aagtacattt tttgttgaat taagtgaaac tgccagcata
     6541 ctcatgcatg caacagcaca ttctctggtg cttgtggatg aattaggaag aggtactgca
     6601 acatttgatg ggacggcaat agcaaatgca gttgttaaag aacttgctga gactataaaa
     6661 tgtcgtacat tattttcaac tcactaccat tcattagtag aagattattc tcaaaatgtt
     6721 gctgtgcgcc taggacatat ggcatgcatg gtagaaaatg aatgtgaaga ccccagccag
     6781 gagactatta cgttcctcta taaattcatt aagggagctt gtcctaaaag ctatggcttt
     6841 aatgcagcaa ggcttgctaa tctcccagag gaagttattc aaaagggaca tagaaaagca
     6901 agagaatttg agaagatgaa tcagtcacta cgattatttc gggaagtttg cctggctagt
     6961 gaaaggtcaa ctgtagatgc tgaagctgtc cataaattgc tgactttgat taaggaatta
     7021 tagggatcca ccggatctag ataactgatc ataattctac cgggtagggg aggcgctttt
     7081 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     7141 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     7201 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     7261 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     7321 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     7381 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     7441 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     7501 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     7561 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7621 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7681 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7741 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7801 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7861 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7921 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7981 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8041 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     8101 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8161 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     8221 gcacgacccc atgcatcggc gtctcgatcg agatatcagt ggtccaggct ctagttttga
     8281 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     8341 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctac
     8401 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
     8461 tcagatcaag gtcaggaaca gatggaacag ggtcgatcga ccctagagaa ccatcagatg
     8521 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     8581 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     8641 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     8701 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     8761 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     8821 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt
     8881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9001 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg
     9061 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9121 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9181 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9361 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat
     9421 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9481 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10741 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11041 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
    11101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    11461 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt
    11521 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa
    11581 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
    11641 cattagttca tagcccatat atggagt
//
