LOCUS       sequence-426792-       10191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AcGFP-PMS2 in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: AcGFP-PMS2
FEATURES             Location/Qualifiers
     source          1..10191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2910
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        5586..6085
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5621..5644)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6018..6037
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6106..6705
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6106..6125)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6602..6622
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6890..7322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7425..7447)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7583..7603)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7588..7917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7768..7903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7939..7958
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8039..8056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8210..8798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8290..8309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8969..9760,9761..9829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9592..9611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9830..9934)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10002..10020
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10120..10191,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcgc cgagctgttc
     2221 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
     2281 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2341 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg
     2401 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
     2461 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg
     2521 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc
     2581 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac
     2641 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc
     2701 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
     2761 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
     2821 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc
     2881 atcacccacg gcatggatga gctgtacaag tccggactca gatccggagg cggaggaagc
     2941 gagcgagctg agagctcgag tacagaacct gctaaggcca tcaaacctat tgatcggaag
     3001 tcagtccatc agatttgctc tgggcaggtg gtactgagtc taagcactgc ggtaaaggag
     3061 ttagtagaaa acagtctgga tgctggtgcc actaatattg atctaaagct taaggactat
     3121 ggagtggatc ttattgaagt ttcagacaat ggatgtgggg tagaagaaga aaacttcgaa
     3181 ggcttaactc tgaaacatca cacatctaag attcaagagt ttgccgacct aactcaggtt
     3241 gaaacttttg gctttcgggg ggaagctctg agctcacttt gtgcactgag cgatgtcacc
     3301 atttctacct gccacgcatc ggcgaaggtt ggaactcgac tgatgtttga tcacaatggg
     3361 aaaattatcc agaaaacccc ctacccccgc cccagaggga ccacagtcag cgtgcagcag
     3421 ttattttcca cactacctgt gcgccataag gaatttcaaa ggaatattaa gaaggagtat
     3481 gccaaaatgg tccaggtctt acatgcatac tgtatcattt cagcaggcat ccgtgtaagt
     3541 tgcaccaatc agcttggaca aggaaaacga cagcctgtgg tatgcacagg tggaagcccc
     3601 agcataaagg aaaatatcgg ctctgtgttt gggcagaagc agttgcaaag cctcattcct
     3661 tttgttcagc tgccccctag tgactccgtg tgtgaagagt acggtttgag ctgttcggat
     3721 gctctgcata atctttttta catctcaggt ttcatttcac aatgcacgca tggagttgga
     3781 aggagttcaa cagacagaca gtttttcttt atcaaccggc ggccttgtga cccagcaaag
     3841 gtctgcagac tcgtgaatga ggtctaccac atgtataatc gacaccagta tccatttgtt
     3901 gttcttaaca tttctgttga ttcagaatgc gttgatatca atgttactcc agataaaagg
     3961 caaattttgc tacaagagga aaagcttttg ttggcagttt taaagacctc tttgatagga
     4021 atgtttgata gtgatgtcaa caagctaaat gtcagtcagc agccactgct ggatgttgaa
     4081 ggtaacttaa taaaaatgca tgcagcggat ttggaaaagc ccatggtaga aaagcaggat
     4141 caatcccctt cattaaggac tggagaagaa aaaaaagacg tgtccatttc cagactgcga
     4201 gaggcctttt ctcttcgtca cacaacagag aacaagcctc acagcccaaa gactccagaa
     4261 ccaagaagga gccctctagg acagaaaagg ggtatgctgt cttctagcac ttcaggtgcc
     4321 atctctgaca aaggcgtcct gagatctcag aaagaggcag tgagttccag tcacggaccc
     4381 agtgacccta cggacagagc ggaggtggag aaggactcgg ggcacggcag cacttccgtg
     4441 gattctgagg ggttcagcat cccagacacg ggcagtcact gcagcagcga gtatgcggcc
     4501 agctccccag gggacagggg ctcgcaggaa catgtggact ctcaggagaa agcgcctgaa
     4561 actgacgact ctttttcaga tgtggactgc cattcaaacc aggaagatac cggatgtaaa
     4621 tttcgagttt tgcctcagcc aactaatctc gcaaccccaa acacaaagcg ttttaaaaaa
     4681 gaagaaattc tttccagttc tgacatttgt caaaagttag taaatactca ggacatgtca
     4741 gcctctcagg ttgatgtagc tgtgaaaatt aataagaaag ttgtgcccct ggacttttct
     4801 atgagttctt tagctaaacg aataaagcag ttacatcatg aagcacagca aagtgaaggg
     4861 gaacagaatt acaggaagtt tagggcaaag atttgtcctg gagaaaatca agcagccgaa
     4921 gatgaactaa gaaaagagat aagtaaaacg atgtttgcag aaatggaaat cattggtcag
     4981 tttaacctgg gatttataat aaccaaactg aatgaggata tcttcatagt ggaccagcat
     5041 gccacggacg agaagtataa cttcgagatg ctgcagcagc acaccgtgct ccaggggcag
     5101 aggctcatag cacctcagac tctcaactta actgctgtta atgaagctgt tctgatagaa
     5161 aatctggaaa tatttagaaa gaatggcttt gattttgtta tcgatgaaaa tgctccagtc
     5221 actgaaaggg ctaaactgat ttccttgcca actagtaaaa actggacctt cggaccccag
     5281 gacgtcgatg aactgatctt catgctgagc gacagccctg gggtcatgtg ccggccttcc
     5341 cgagtcaagc agatgtttgc ctccagagcc tgccggaagt cggtgatgat tgggactgct
     5401 ctcaacacaa gcgagatgaa gaaactgatc acccacatgg gggagatgga ccacccctgg
     5461 aactgtcccc atggaaggcc aaccatgaga cacatcgcca acctgggtgt catttctcag
     5521 aactgagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatt
     5581 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     5641 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     5701 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     5761 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     5821 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     5881 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     5941 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6001 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6061 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     6121 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6181 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6241 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6301 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6361 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6421 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6481 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6541 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6601 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6661 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     6721 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggcgtctcg atcgagatat
     6781 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
     6841 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     6901 ccacctgtag gtttggcaag ctactagctt aagtaacgcc attttgcaag gcatggaaaa
     6961 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga
     7021 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     7081 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     7141 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     7201 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     7261 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     7321 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     7381 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7441 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7501 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     7561 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7621 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7681 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7741 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7801 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7861 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7921 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     7981 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     8041 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8641 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8881 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8941 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9001 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9061 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9121 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9181 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9241 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     9301 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9361 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9421 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9481 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9541 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     9601 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9661 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9721 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9781 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9841 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9961 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10021 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
    10081 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
    10141 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t
//
