LOCUS       sequence-426793-       10105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EXO1B-AcGFP in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: EXO1B-AcGFP
FEATURES             Location/Qualifiers
     source          1..10105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2179..2188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4753..5472
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        5500..5999
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5535..5558)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5932..5951
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6020..6619
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6020..6039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6516..6536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6804..7236
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7339..7361)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7497..7517)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7502..7831
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7682..7817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7853..7872
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7953..7970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8124..8712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8204..8223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8883..9674,9675..9743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9506..9525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9744..9848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9916..9934
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10034..10105,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagccgc caccatgggg atacagggat tgctacaatt tatcaaagaa
     2221 gcttcagaac ccatccatgt gaggaagtat aaagggcagg tagtagctgt ggatacatat
     2281 tgctggcttc acaaaggagc tattgcttgt gctgaaaaac tagccaaagg tgaacctact
     2341 gataggtatg taggattttg tatgaaattt gtaaatatgt tactatctca tgggatcaag
     2401 cctattctcg tatttgatgg atgtacttta ccttctaaaa aggaagtaga gagatctaga
     2461 agagaaagac gacaagccaa tcttcttaag ggaaagcaac ttcttcgtga ggggaaagtc
     2521 tcggaagctc gagagtgttt cacccggtct atcaatatca cacatgccat ggcccacaaa
     2581 gtaattaaag ctgcccggtc tcagggggta gattgcctcg tggctcccta tgaagctgat
     2641 gcgcagttgg cctatcttaa caaagcggga attgtgcaag ccataattac agaggactcg
     2701 gatctcctag cttttggctg taaaaaggta attttaaaga tggaccagtt tggaaatgga
     2761 cttgaaattg atcaagctcg gctaggaatg tgcagacagc ttggggatgt attcacggaa
     2821 gagaagtttc gttacatgtg tattctttca ggttgtgact acctgtcatc actgcgtggg
     2881 attggattag caaaggcatg caaagtccta agactagcca ataatccaga tatagtaaag
     2941 gttatcaaga aaattggaca ttatctcaag atgaatatca cggtaccaga ggattacatc
     3001 aacgggttta ttcgggccaa caataccttc ctctatcagc tagtttttga tcccatcaaa
     3061 aggaaactta ttcctctgaa cgcctatgaa gatgatgttg atcctgaaac actaagctac
     3121 gctgggcaat atgttgatga ttccatagct cttcaaatag cacttggaaa taaagatata
     3181 aatacttttg aacagatcga tgactacaat ccagacactg ctatgcctgc ccattcaaga
     3241 agtcgtagtt gggatgacaa aacatgtcaa aagtcagcta atgttagcag catttggcat
     3301 aggaattact ctcccagacc agagtcgggt actgtttcag atgccccaca attgaaggaa
     3361 aatccaagta ctgtgggagt ggaacgagtg attagtacta aagggttaaa tctcccaagg
     3421 aaatcatcca ttgtgaaaag accaagaagt gcagagctgt cagaagatga cctgttgagt
     3481 cagtattctc tttcatttac gaagaagacc aagaaaaata gctctgaagg caataaatca
     3541 ttgagctttt ctgaagtgtt tgtgcctgac ctggtaaatg gacctactaa caaaaagagt
     3601 gtaagcactc cacctaggac gagaaataaa tttgcaacat ttttacaaag gaaaaatgaa
     3661 gaaagtggtg cagttgtggt tccagggacc agaagcaggt ttttttgcag ttcagattct
     3721 actgactgtg tatcaaacaa agtgagcatc cagcctctgg atgaaactgc tgtcacagat
     3781 aaagagaaca atctgcatga atcagagtat ggagaccaag aaggcaagag actggttgac
     3841 acagatgtag cacgtaattc aagtgatgac attccgaata atcatattcc aggtgatcat
     3901 attccagaca aggcaacagt gtttacagat gaagagtcct actcttttaa gagcagcaaa
     3961 tttacaagga ccatttcacc acccactttg ggaacactaa gaagttgttt tagttggtct
     4021 ggaggtcttg gagatttttc aagaacgccg agcccctctc caagcacagc attgcagcag
     4081 ttccgaagaa agagcgattc ccccacctct ttgcctgaga ataatatgtc tgatgtgtcg
     4141 cagttaaaga gcgaggagtc cagtgacgat gagtctcatc ccttacgaga aggggcatgt
     4201 tcttcacagt cccaggaaag tggagaattc tcactgcaga gttcaaatgc atcaaagctt
     4261 tctcagtgct ctagtaagga ctctgattca gaggaatctg attgcaatat taagttactt
     4321 gacagtcaaa gtgaccagac ctccaagcta tgtttatctc atttctcaaa aaaagacaca
     4381 cctctaagga acaaggttcc tgggctatat aagtccagtt ctgcagactc tctttctaca
     4441 accaagatca aacctctagg acctgccaga gccagtgggc tgagcaagaa gccggcaagc
     4501 atccagaaga gaaagcatca taatgccgag aacaagccgg ggttacagat caaactcaat
     4561 gagctctgga aaaactttgg atttaaaaaa gattctgaaa agcttcctcc ttgtaagaaa
     4621 cccctgtccc cagtcagaga taacatccaa ctaactccag aagcggaaga ggatatattt
     4681 aacaaacctg aatgtggccg tgttcaaaga gcaatattcc agggaggcgg aggaagcggg
     4741 gatccaccgg tcatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg
     4801 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc
     4861 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg
     4921 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc
     4981 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag
     5041 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag
     5101 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac
     5161 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac
     5221 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc
     5281 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg
     5341 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc
     5401 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag
     5461 ctgtacaagt gagcggccgc gactctagat aattctaccg ggtaggggag gcgcttttcc
     5521 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     5581 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     5641 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     5701 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     5761 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     5821 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     5881 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     5941 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     6001 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6061 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6121 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6181 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6241 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6301 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6361 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6421 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6481 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6541 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6601 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     6661 acgaccccat gcatcggcgt ctcgatcgag atatcagtgg tccaggctct agttttgact
     6721 caacaatatc accagctgaa gcctatagag tacgagccat agataaaata aaagatttta
     6781 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctacta
     6841 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     6901 agatcaaggt caggaacaga tggaacaggg tcgatcgacc ctagagaacc atcagatgtt
     6961 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     7021 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     7081 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     7141 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     7201 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag
     7261 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt
     7321 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     7381 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     7441 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa
     7501 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7561 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7621 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7681 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7741 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7801 aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca
     7861 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     7921 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8521 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9181 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9481 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     9541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9841 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     9901 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attagcttgg
     9961 ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca
    10021 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
    10081 ttagttcata gcccatatat ggagt
//
