LOCUS       sequence-426799-       10323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-LIG1 in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: mCherry-LIG1
FEATURES             Location/Qualifiers
     source          1..10323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2901
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2339..2357)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2607..2626
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5718..6217
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5753..5776)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6150..6169
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6238..6837
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6238..6257)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6734..6754
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7022..7454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7557..7579)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7715..7735)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7720..8049
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7900..8035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8071..8090
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8171..8188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8342..8930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8422..8441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9101..9892,9893..9961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9724..9743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9962..10066)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10134..10152
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10252..10323,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2881 ggcatggacg agctgtacaa gtccggactc agatctcgag gaggcggagg aagccagcga
     2941 agtatcatgt catttttcca ccccaagaaa gagggtaaag caaagaagcc tgagaaggag
     3001 gcatccaata gcagcagaga gacggagccc cctccaaagg cggcactgaa ggagtggaat
     3061 ggagtggtgt ccgagagtga ctctccggtg aagaggccag ggaggaaggc ggcccgggtc
     3121 ctgggcagcg aaggggaaga ggaggatgaa gcccttagcc ctgctaaagg ccagaagcct
     3181 gccctggact gctcacaggt ctccccgccc cgtcctgcca catctcctga gaacaatgct
     3241 tccctctctg acacctctcc catggacagt tccccatcag ggattccgaa gcgtcgcaca
     3301 gctcggaagc agctcccgaa acggaccatt caggaagtcc tggaagagca gagtgaggac
     3361 gaggacagag aagccaagag gaagaaggag gaggaagaag aggagacccc gaaagaaagc
     3421 ctcacagagg ctgaagtggc cacagagaag gaaggagaag acggggacca gcccaccacg
     3481 cctcccaagc ccctaaagac ctccaaagca gagaccccga cggaaagcgt ttcagagcct
     3541 gaggtggcca cgaagcagga actgcaggag gaggaagagc agaccaagcc tccccgcaga
     3601 gctcccaaga cgctcagcag cttcttcacc ccccggaagc cagcagtcaa aaaagaagtg
     3661 aaggaagagg agccaggggc tccaggaaag gagggagctg ctgagggacc cctggatcca
     3721 tctggttaca atcctgccaa gaacaactat catcccgtgg aagatgcctg ctggaaaccg
     3781 ggccagaagg ttccttacct ggctgtggcc cggacgtttg agaagatcga ggaggtgtct
     3841 gctcggctcc ggatggtgga gacgctgagc aacttgctgc gctccgtggt ggccctgtcg
     3901 cctccagacc tcctccctgt cctctacctc agcctcaacc accttgggcc accccagcag
     3961 ggcctggagc ttggcgtggg tgatggtgtc cttctcaagg cagtggccca ggccacaggt
     4021 cggcagctgg agtccgtccg ggctgaggca gccgagaaag gcgacgtggg gctggtggcc
     4081 gagaacagcc gcagcaccca gaggctcatg ctgccaccac ctccgctcac tgcctccggg
     4141 gtcttcagca agttccgcga catcgccagg ctcactggca gtgcttccac agccaagaag
     4201 atagacatca tcaaaggcct ctttgtggcc tgccgccact cagaagcccg gttcatcgct
     4261 aggtccctga gcggacggct gcgccttggg ctggcagagc agtcggtgct ggctgccctc
     4321 tcccaggcag tgagcctcac gcccccgggc caagaattcc caccagccat ggtggatgct
     4381 gggaagggca agacagcaga ggccagaaag acgtggctgg aggagcaagg catgatcctg
     4441 aagcagacgt tctgcgaggt tcccgacctg gaccgaatta tccccgtgct gctggagcac
     4501 ggcctggaac gtctcccgga gcactgcaag ctgagcccag ggattcccct gaaaccaatg
     4561 ttggcccatc ccacccgggg catcagcgag gtcctgaaac gctttgagga ggcagctttc
     4621 acctgcgaat acaaatatga cgggcagagg gcacagatcc acgccctgga aggcggggag
     4681 gtgaagatct tcagcaggaa tcaggaagac aacactggga agtacccgga catcatcagc
     4741 cgcatcccca agattaaact cccatcggtc acatccttca tcctggacac cgaagccgtg
     4801 gcttgggacc gggaaaagaa gcagatccag ccattccaag tgctcaccac ccgcaaacgc
     4861 aaggaggtgg atgcgtctga gatccaggtg caggtgtgtt tgtacgcctt cgacctcatc
     4921 tacctcaatg gagagtccct ggtacgtgag cccctttccc ggcgccggca gctgctccgg
     4981 gagaactttg tggagacaga gggcgagttt gtcttcgcca cctccctgga caccaaggac
     5041 atcgagcaga tcgccgagtt cctggagcag tcagtgaaag actcctgcga ggggctgatg
     5101 gtgaagaccc tggatgttga tgccacctac gagatcgcca agagatcgca caactggctc
     5161 aagctgaaga aggactacct tgatggcgtg ggtgacaccc tggacctggt ggtgatcggc
     5221 gcctacctgg gccgggggaa gcgggccggc cggtacgggg gcttcctgct ggcctcctac
     5281 gacgaggaca gtgaggagct gcaggccata tgcaagcttg gaactggctt cagtgatgag
     5341 gagctggagg agcatcacca gagcctcaag gcgctggtgc tgcccagccc acgcccttac
     5401 gtgcggatag atggcgctgt gattcccgac cactggctgg accccagcgc tgtgtgggag
     5461 gtgaagtgcg ctgacctctc cctctctccc atctaccctg ctgcgcgggg cctggtggat
     5521 agtgacaagg gcatctccct tcgcttccct cggtttattc gagtccgtga agacaagcag
     5581 ccggagcagg ccaccaccag tgctcaggtg gcctgtttgt accggaagca aagtcagatt
     5641 cagaaccaac aaggcgagga ctcaggctct gaccctgaag atacctactg atctagataa
     5701 ctgatcataa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     5761 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     5821 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     5881 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     5941 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     6001 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     6061 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     6121 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     6181 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     6241 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6301 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6361 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6421 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6481 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6541 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6601 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6661 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6721 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6781 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6841 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggcgtct
     6901 cgatcgagat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     6961 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     7021 gaatgaaaga ccccacctgt aggtttggca agctactagc ttaagtaacg ccattttgca
     7081 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     7141 gaacagggtc gatcgaccct agagaaccat cagatgtttc cagggtgccc caaggacctg
     7201 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     7261 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     7321 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     7381 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     7441 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
     7501 cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7561 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7621 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7681 tcacgtagcg atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga
     7741 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7801 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7861 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7981 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8041 ttttgcaaaa agcttactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8101 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     8161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8761 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     9421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     9721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10081 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10141 gtatcacgag gccctttcgt cttcaagaat tagcttggcc attgcatacg ttgtatccat
    10201 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
    10261 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10321 agt
//
