LOCUS       sequence-426804-       14451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-POLE1 in mammalian cells from a retroviral 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14451)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 14451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: mCherry-POLE1
FEATURES             Location/Qualifiers
     source          1..14451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2901
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2339..2357)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2607..2626
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        9846..10345
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9881..9904)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10278..10297
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10366..10965
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10366..10385)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10862..10882
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11150..11582
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(11685..11707)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11843..11863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11848..12177
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12028..12163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12199..12218
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12299..12316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12470..13058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12550..12569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13229..14020,14021..14089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13852..13871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14090..14194)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14262..14280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(14380..14451,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2881 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattctgca
     2941 gtcgacggag gcggaggaag ctctctgagg agcggcgggc ggcggcgcgc ggacccaggc
     3001 gcggatggcg aggccagcag ggatgatggc gccacttcct cagtttcggc actcaagcgc
     3061 ctggaacgga gtcagtggac ggataagatg gatttgcggt ttggttttga gcggctgaag
     3121 gagcctggtg agaagacagg ctggctcatt aacatgcatc ctaccgagat tttagatgaa
     3181 gataagcgct taggcagtgc ggtagactat tatttcatcc aagatgacgg aagcagattt
     3241 aaggtggctt tgccctataa accgtatttc tacattgcga ccagaaaggg ttgtgagcga
     3301 gaagtttcat cttttctctc caagaagttt cagggcaaaa ttgcaaaagt ggagactgtc
     3361 cccaaagagg atctggactt gccaaatcac ttggtgggtt tgaagcgaaa ttacatcagg
     3421 ctgtccttcc acactgtgga ggatcttgtc aaagtgagga aggagatctc ccctgccgtg
     3481 aagaagaaca gggagcagga tcacgccagc gacgcgtaca cagctctgct ttccagtgtt
     3541 ctgcagaggg gcggtgtcat tactgatgaa gaggaaacct ctaagaagat agccgatcaa
     3601 ctagataata tcgtggacat gcgcgagtac gatgttccct accacatccg cctctccatt
     3661 gacctgaaga tccacgtggc tcattggtac aatgtcagat accgaggaaa tgcttttccg
     3721 gtagaaatca cccgccgaga tgaccttgtt gaacgacctg accctgtggt tttggcattt
     3781 gacattgaga cgaccaaact gcccctcaag tttcctgatg ctgagacaga ccagattatg
     3841 atgatttcct acatgatcga tggccagggc tacctcatca ccaacaggga gattgtttca
     3901 gaagatattg aagattttga gttcaccccc aagccagaat atgaaggccc cttttgtgtc
     3961 ttcaatgaac ccgatgaggc tcatctgatc caaaggtggt ttgaacacgt ccaggagacc
     4021 aaacccacca tcatggtcac ctacaacggg gacttttttg actggccatt tgtggaggcc
     4081 cgggcagcag tccacggtct gagcatgcag caggagatag gcttccagaa ggacagccag
     4141 ggggagtaca aggcgcccca gtgcatccac atggactgcc tcaggtgggt gaagagggac
     4201 agttaccttc ctgtgggcag tcataatctc aaggcggccg ccaaggccaa gctaggctat
     4261 gatcccgtgg agctagaccc ggaggacatg tgccggatgg ccacggagca gccccagact
     4321 ctggccacgt attctgtgtc agatgctgtc gccacttact acctgtacat gaagtacgtc
     4381 cacccattca tctttgctct gtgcaccatt attcccatgg agcccgacga ggtgctgcgg
     4441 aagggctctg gcactctgtg tgaggccttg ctgatggtgc aggccttcca cgccaacatc
     4501 atcttcccca acaagcaaga gcaggagttc aataagctga cggacgacgg acacgtgctg
     4561 gactctgaga cctacgtcgg gggccacgtg gaggccctcg agtctggggt tttccgcagc
     4621 gatatccctt gccggtttag gatgaatcct gccgcctttg acttcctgct gcagcgggtt
     4681 gagaagacct tgcgccacgc ccttgaggaa gaggagaaag tgcctgtgga gcaagtcacc
     4741 aactttgaag aggtgtgtga tgagattaag agcaagcttg cctccctgaa ggacgttccc
     4801 agccgcatcg agtgtccact catctaccac ctggacgtgg gggccatgta ccccaacatc
     4861 atcctgacca accgcctgca gccctctgcc atggtggacg aagccacctg tgctgcctgt
     4921 gacttcaata agcctggagc aaactgccag cggaagatgg cctggcagtg gaggggcgag
     4981 ttcatgccag ccagtcgcag cgaataccat cggatccagc accagctgga gtcagagaag
     5041 ttccccccct tgttcccaga ggggccagct cgggcctttc atgaactgtc ccgcgaggaa
     5101 caggcgaaat acgagaagag aaggctggcg gattactgcc ggaaagccta caagaagatc
     5161 cacatcacca aggtggaaga gcgtctcacc accatctgcc agcgggaaaa ctccttctac
     5221 gtggacaccg tgcgtgcctt ccgggacagg cgttacgagt tcaaagggct ccacaaggtg
     5281 tggaaaaaga agctctcggc ggccgtggag gtgggcgacg cggctgaggt gaagcgctgc
     5341 aagaacatgg aggtgctgta tgactcgctg cagctggccc acaagtgcat cctgaactcc
     5401 ttctatggct atgtcatgcg caagggggct cgctggtact ccatggagat ggctggcatc
     5461 gtctgcttca caggggccaa catcatcacc caggcacggg agctgatcga gcagattggg
     5521 aggcccttag agctggacac agatggtata tggtgcgtcc tgcccaacag cttcccagaa
     5581 aattttgtct tcaagacgac caatgtgaag aagcccaaag tgaccatctc ctacccaggc
     5641 gccatgttga acatcatggt caaggaaggc ttcaccaatg accagtacca ggagctggct
     5701 gagccgtcct cactcaccta cgtcacccgc tcagagaaca gcatcttttt tgaggttgat
     5761 gggccctacc ttgccatgat tcttccagcc tccaaggaag aaggcaagaa attgaagaag
     5821 aggtatgctg tgttcaatga agacggttct ctggctgagc tcaagggctt tgaggtcaaa
     5881 cgccgcgggg aactgcagct gattaagatc ttccaatcct cggtgtttga ggccttcctc
     5941 aagggcagca cgctggaaga ggtgtatggc tctgtagcca aggtggctga ctactggctg
     6001 gacgtgctgt acagcaaggc agccaacatg cctgactctg agctattcga gctcatctct
     6061 gagaaccgtt ccatgtctcg gaagctggaa gattacgggg agcagaagtc tacgtccatc
     6121 agcacagcaa agcgcctggc cgagttcctg ggagaccaga tggtcaagga tgcagggctg
     6181 agttgccgct acatcatctc ccgcaagccc gagggctccc ctgtcacgga gagggccatc
     6241 ccacttgcca ttttccaagc agagcccacg gtgaggaagc actttctccg gaaatggctc
     6301 aagagctctt cccttcaaga ctttgatatt cgagcaattc tggattggga ctactacatt
     6361 gagcggctgg gaagcgccat ccagaagatc atcaccatcc ctgcggccct gcagcaggta
     6421 aagaacccag tgccacgtgt caaacacccc gactggctgc acaaaaaact gctggagaag
     6481 aatgatgtct acaagcagaa gaagatcagt gagctcttca ccctggaggg caggagacag
     6541 gtcacgatgg ccgaggcctc agaagacagt ccgaggccaa gtgctcctga catggaggac
     6601 ttcggcctcg taaagctgcc tcacccagca gcccctgtca ctgtgaagag gaagcgagtt
     6661 ctttgggaga gccaggagga gtcccaggac ctcacgccga ctgtgccctg gcaggaaatc
     6721 ttggggcagc ctcccgccct gggaaccagc caggaggaat ggcttgtctg gctccggttc
     6781 cacaagaaga agtggcagct gcaggcccgg cagcgcctcg cccgcaggaa gaggcagcgt
     6841 ctggagtcgg cagagggtgt gctcaggccc ggggccatcc gggatggtcc tgccacgggg
     6901 ctggggagct tcttgcgaag aactgcccgc agcatcctgg accttccgtg gcagattgtg
     6961 cagatcagcg agaccagcca ggccggcctg ttcaggctgt gggcgctcgt tggcagtgac
     7021 ttgcactgca tcaggctgag catcccccgt gtgttctacg tgaaccagcg agtcgctaaa
     7081 gcggaggagg gtgcttcgta tcgcaaggta aatcgggtcc ttcctcgctc caacatggtc
     7141 tacaatctct atgagtattc agtgccagag gacatgtacc aggaacacat caacgagatc
     7201 aacgctgagc tgtcagcgcc agacatcgag ggcgtatatg agactcaggt tccgttactg
     7261 ttccgggccc tggtgcacct gggctgtgtg tgtgtggtca ataaacagct ggtgaggcac
     7321 ctttcaggct gggaagcaga gacctttgct cttgagcacc tggagatgcg ctctctggcc
     7381 cagttcagct acctggaacc agggagtatc cgccatatct acctgtacca ccacgcacag
     7441 gcccacaaag cgctcttcgg gatcttcatc ccctcacagc gcagggcatc cgtctttgtg
     7501 ctggacactg tgcgcagcaa ccagatgccc agccttggcg ccctgtactc agcagagcac
     7561 ggcctcctcc tggagaaggt gggccctgag ctcctgccac cccccaaaca caccttcgaa
     7621 gttcgggcag aaactgacct gaagaccatc tgcagagcca tccagcgatt cctgctcgcc
     7681 tacaaggagg agcgccgggg gcccacactc atcgctgttc agtccagctg ggagctgaag
     7741 aggctggcca gtgaaattcc tgtcttggag gaattcccac tggtgcctat ctgtgtggct
     7801 gacaagatca actatggggt cctggactgg cagcgccatg gagcccggcg catgatccgt
     7861 cactacctca acctggacac ctgcctgtcg caggccttcg agatgagcag gtactttcac
     7921 attcccattg ggaacctacc agaggacatc tccacattcg gctccgacct cttctttgcc
     7981 cgccacctcc agcgccacaa ccacctgctc tggctgtccc ctacagcccg ccctgacctg
     8041 ggtggaaagg aggctgatga caactgtctt gtcatggagt tcgatgacca agccactgtt
     8101 gagatcaaca gttcaggctg ttactccaca gtgtgtgtgg agctggacct tcagaacctg
     8161 gccgtcaaca ccattctcca gtctcaccat gtcaacgaca tggagggggc cgacagcatg
     8221 gggatcagct tcgacgtgat ccagcaggcc tccctggagg acatgatcac gggtggtcag
     8281 gctgccagtg ccccggccag ctacgatgag acagccctgt gctctaacac cttcaggatc
     8341 ctgaagagca tggtcgtggg ctgggtgaag gagatcaccc agtaccacaa catctatgca
     8401 gacaaccagg tgatgcactt ctaccgctgg cttcggtcgc catcctctct gcttcatgac
     8461 cctgccctgc accgcacact ccacaacatg atgaagaagc tcttcctgca gctcatcgct
     8521 gagttcaagc gcctggggtc atcagtcatc tacgccaact tcaaccgcat catcctctgt
     8581 acaaagaagc gccgtgtgga agatgccatc gcttacgtgg agtacatcac cagcagcatc
     8641 cattcaaagg agaccttcca ttctctgaca atttctttct ctcgatgctg ggaatttctt
     8701 ctctggatgg atccatctaa ctatggcgga atcaaaggaa aagtttcatc tcgtattcac
     8761 tgtggactgc aagactccca gaaagcaggg ggagcagagg atgagcagga aaatgaggac
     8821 gatgaggagg aaagagatgg ggaggaggag gaagaggcgg aggaatccaa cgtggaggat
     8881 ttactggaaa acaactggaa cattttgcag tttttgccac aggcagcctc ctgccagaac
     8941 tacttcctca tgattgtttc agcgtacatc gtggccgtgt accactgcat gaaggacggg
     9001 ctgaggcgca gtgctccagg gagcaccccc gtgaggagga ggggggccag ccagctctcc
     9061 caggaggccg agggggcggt cggagccctt cccggaatga tcaccttctc tcaggattat
     9121 gtcgcaaatg agctcactca gagcttcttc accatcactc agaagattca gaagaaagtc
     9181 acaggctctc ggaactccac tgagctctca gagatgtttc ctgtcctccc cggttcccac
     9241 ttgctgctca ataaccctgc cctggagttc atcaaatacg tgtgcaaggt gctgtccctg
     9301 gacaccaaca tcacaaacca ggtgaataag ctgaaccgag acctgcttcg cctggtggat
     9361 gtcggcgagt tctccgagga ggcccagttc cgagacccct gccgctccta cgtgcttcct
     9421 gaggtcatct gccgcagctg taacttctgc cgcgacctgg acctgtgtaa agactcttcc
     9481 ttctcagagg atggggcggt cctgcctcag tggctctgct ccaactgtca ggcgccctac
     9541 gactcctctg ccatcgagat gacgctggtg gaagttctac agaagaagct gatggccttc
     9601 accctgcagg acctggtctg cctgaagtgc cgcggggtga aggagaccag catgcctgtg
     9661 tactgcagct gcgcgggaga cttcgccctc accatccaca cccaggtctt catggaacag
     9721 atcggaatat tccggaacat tgcccagcac tacggcatgt cgtacctcct ggagaccctg
     9781 gagtggctgc tgcagaagaa cccacagctg ggccattagt ctagataact gatcataatt
     9841 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     9901 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     9961 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
    10021 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
    10081 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
    10141 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
    10201 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
    10261 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
    10321 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
    10381 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
    10441 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
    10501 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
    10561 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
    10621 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
    10681 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
    10741 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
    10801 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
    10861 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
    10921 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
    10981 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggcgtctcg atcgagatat
    11041 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
    11101 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
    11161 ccacctgtag gtttggcaag ctactagctt aagtaacgcc attttgcaag gcatggaaaa
    11221 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga
    11281 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
    11341 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
    11401 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
    11461 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
    11521 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
    11581 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
    11641 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    11701 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    11761 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
    11821 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    11881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    11941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    12001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    12061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    12121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    12181 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    12241 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    12301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    13141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    13201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    13261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    13321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    13381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    13441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    13501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
    13561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    13621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    13681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    13741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    13801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
    13861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    13921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    13981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    14041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    14101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    14161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    14221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    14281 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
    14341 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
    14401 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t
//
