LOCUS       sequence-426805-       10593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mNeonGreen-LIG3 in mammalian cells from a retroviral 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: mNeonGreen-LIG3
FEATURES             Location/Qualifiers
     source          1..10593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2901
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      2449..2458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3731..3740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5988..6487
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6023..6046)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6420..6439
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6508..7107
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6508..6527)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7004..7024
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7292..7724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7827..7849)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7985..8005)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7990..8319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8170..8305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8341..8360
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8441..8458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8612..9200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8692..8711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9371..10162,10163..10231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9994..10013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10232..10336)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10404..10422
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10522..10593,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
     2221 atggcctctc tcccagcgac acatgagtta cacatctttg gctccatcaa cggtgtggac
     2281 tttgacatgg tgggtcaggg caccggcaat ccaaatgatg gttatgagga gttaaacctg
     2341 aagtccacca agggtgacct ccagttctcc ccctggattc tggtccctca tatcgggtat
     2401 ggcttccatc agtacctgcc ctaccctgac gggatgtcgc ctttccaggc cgccatggta
     2461 gatggctccg gctaccaagt ccatcgcaca atgcagtttg aagatggtgc ctcccttact
     2521 gttaactacc gctacaccta cgagggaagc cacatcaaag gagaggccca ggtgaagggg
     2581 actggtttcc ctgctgacgg tcctgtgatg accaactcgc tgaccgctgc ggactggtgc
     2641 aggtcgaaga agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac
     2701 accactggaa atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag
     2761 ccaatggcgg ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc
     2821 aagcactcca agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg
     2881 ggcatggacg agctgtacaa gtccggactc agatctcgac catctttggc tttcaagatc
     2941 ttctttccac aaaccctccg tgcactcagc cgaaaagaac tgtgcctatt ccgaaaacat
     3001 cactggcgtg atgtaagaca attcagccag tggtcagaaa cagatctgct tcatggacat
     3061 cccctcttcc tgagaagaaa gcctgttcta tcattccagg gaagccatct aagatcacgt
     3121 gccacctacc ttgttttctt gccagggttg catgtgggac tctgcagtgg cccctgtgag
     3181 atggctgagc aacggttctg tgtggactat gccaagcgtg gcacagctgg ctgcaaaaaa
     3241 tgcaaggaaa agattgtgaa gggcgtatgc cgaattggca aagtggtgcc caatcccttc
     3301 tcagagtctg ggggtgatat gaaagagtgg taccacatta aatgcatgtt tgagaaacta
     3361 gagcgggccc gggccaccac aaaaaaaatc gaggacctca cagagctgga aggctgggaa
     3421 gagctggaag ataatgagaa ggaacagata acccagcaca ttgcagatct gtcttctaag
     3481 gcagcaggta caccaaagaa gaaagctgtt gtccaggcta agttgacaac cactggccag
     3541 gtgacttctc cagtgaaagg cgcctcattt gtcaccagta ccaatccccg gaaattttct
     3601 ggcttttcag ccaagcccaa caactctggg gaagccccct cgagccccac ccctaagaga
     3661 agtctgtctt caagcaaatg tgaccccagg cataaggact gtctgctacg ggagtttcga
     3721 aagttatgcg ccatggtggc cgataatcct agctacaaca cgaagaccca gatcatccag
     3781 gacttccttc ggaaaggctc agcaggagat ggtttccacg gtgatgtgta cctaacagtg
     3841 aagctgctgc tgccaggagt cattaagact gtttacaact tgaacgataa gcagattgtg
     3901 aagcttttca gtcgcatttt taactgcaac ccagatgata tggcacggga cctagagcag
     3961 ggtgacgtgt cagagacaat cagagtcttc tttgagcaga gcaagtcttt ccccccagct
     4021 gccaagagcc tccttaccat ccaggaagtg gatgagttcc ttctgcggct gtccaagctc
     4081 accaaggagg atgagcagca acaggcccta caggacattg cctccaggtg tacagccaat
     4141 gaccttaaat gcatcatcag gttgatcaaa catgatctga agatgaactc aggtgcaaaa
     4201 catgtgttag acgcccttga ccccaatgcc tatgaagcct tcaaagcctc gcgcaacctg
     4261 caggatgtgg tggagcgggt ccttcacaac gcgcaggagg tggagaagga gccgggccag
     4321 agacgagctc tgagcgtcca ggcctcgctg atgacacctg tgcagcccat gttggcggag
     4381 gcctgcaagt ccgttgagta tgcaatgaag aaatgtccca atggcatgtt ctctgagatc
     4441 aagtacgatg gagagcgagt ccaggtgcat aagaatggag accacttcag ctacttcagc
     4501 cgcagtctca agcccgtcct tcctcacaag gtggcccact ttaaggacta cattccccag
     4561 gcttttcctg ggggccacag catgatcttg gattctgaag tgcttctgat tgacaacaag
     4621 acaggcaaac cactgccctt tgggactctg ggagtacaca agaaagcagc cttccaggat
     4681 gctaatgtct gcctgtttgt ttttgattgt atctacttta atgatgtcag cttgatggac
     4741 agacctctgt gtgagcggcg gaagtttctt catgacaaca tggttgaaat tccaaaccgg
     4801 atcatgttct cagaaatgaa gcgagtcaca aaagctttgg acttggctga catgataacc
     4861 cgggtgatcc aggagggatt ggaggggctg gtgctgaagg atgtgaaggg tacatatgag
     4921 cctgggaagc ggcactggct gaaagtgaag aaagactatt tgaacgaggg ggccatggcc
     4981 gacacagctg acctggtggt ccttggagcc ttctatgggc aagggagcaa aggcggcatg
     5041 atgtcaatct tcctcatggg ctgctacgac cctggcagcc agaagtggtg cacagtcacc
     5101 aagtgtgcag gaggccatga tgatgccacg cttgcccgcc tgcagaatga actagacatg
     5161 gtgaagatca gcaaggaccc cagcaaaata cccagctggt tgaaggtcaa caagatctac
     5221 tatcctgact tcatcgtccc agacccaaag aaagctgccg tgtgggagat cacaggggct
     5281 gaattctcca aatcggaggc tcatacagct gacgggatct ccatccgatt ccctcgctgc
     5341 acccgaatcc gagatgataa ggactggaaa tctgccacta accttcccca actcaaggaa
     5401 ctgtaccagt tgtccaagga gaaggcagac ttcactgtag tggctggaga tgaggggagc
     5461 tccactacag ggggtagcag tgaagagaat aagggtccct cagggtctgc tgtgtcccgc
     5521 aaggccccca gcaagccctc agccagtacc aagaaagcag aagggaagct gagtaactcc
     5581 aacagcaaag atggcaacat gcagactgca aagccttccg ctatgaaggt gggggagaag
     5641 ctggccacaa agtcttctcc agtgaaagta ggggagaagc ggaaagctgc tgatgagacg
     5701 ctgtgccaaa caaaggtatt gctggacatc ttcactgggg tgcggcttta cttgccaccc
     5761 tccacaccag acttcagccg tctcagacgc tactttgtgg cattcgacgg ggacctggta
     5821 caggaatttg atatgacttc agccacgcac gtgctgggta gcagggacaa gaaccctgcg
     5881 gcccagcagg tctccccaga gtggatttgg gcatgtatcc ggaaacggag actggtagct
     5941 ccctgctagg gatccaccgg atctagataa ctgatcataa ttctaccggg taggggaggc
     6001 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     6061 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     6121 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     6181 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6241 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6301 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6361 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6421 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6481 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6541 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6601 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6661 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6721 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6781 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6841 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6901 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6961 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7021 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7081 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     7141 aggagcgcac gaccccatgc atcggcgtct cgatcgagat atcagtggtc caggctctag
     7201 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa
     7261 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     7321 agctactagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     7381 agaagttcag atcaaggtca ggaacagatg gaacagggtc gatcgaccct agagaaccat
     7441 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     7501 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     7561 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     7621 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     7681 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
     7741 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggctgcctc
     7801 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7861 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7921 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtagatccgg
     7981 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8041 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8161 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8221 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8281 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactgg cttaactatg
     8341 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     8401 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     8461 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8521 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8581 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8641 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8701 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8761 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8821 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8881 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8941 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9001 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9061 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9121 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9181 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9241 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9301 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9361 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9661 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     9721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9961 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
    10021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10321 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10381 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
    10441 tagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
    10501 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
    10561 cggggtcatt agttcatagc ccatatatgg agt
//
