LOCUS       sequence-42681-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU
  TITLE     Double conditional human embryonic kidney cell line based on FLP and
            PhiC31 mediated transgene integration.
  JOURNAL   BMC Res Notes. 2011 Oct 18;4:420.
  PUBMED    22008483
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2011-10-18"; volume: "4"; pages: "420"
COMMENT     Sequence Label: pINT-PuroDDHNF4a2
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    117..251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(154..173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     208..227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aagggaacaa aagctgggta ccgggcccaa tgcattggcg ccgcggccgc gaattaaaaa
       61 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      241 atgtctggat ctacgtaata cgactcacta tagttctaga ccctgcctgg tgcctggtcc
      301 cagtcagtgc ttacaacatg ttcccagaat gactggggga atcgtttcca aggcctcctt
      361 ggcctctgta gaggtggtgt tggagaagac gggatggctg agcaaggtga cagcagaggg
      421 aggcagcaga ggtgggggca gcagcagaag gaggagcaag gtgccccaag tgccacaatt
      481 ggacccagcc tcacagggct ctgtctggac accatgaccg gggctaggag aggagaagca
      541 ccaggctagg gaagaaggga gctgggttta agtaaggcct caagtcatct ccaggcggct
      601 gtcctttggg atgaagtcgg gggttggaca tggatgaagg tgaaggtgaa ggcagtggct
      661 tcaacatgag aaaagttgtc caaggcagta gaggtctccc caagtcaaag tcttgttatc
      721 cagagcaggg cgtcaagggt ggcagtggga tgtggccctt aggccatgtt ctcgggccct
      781 tcatccttcc cattcctgct ctgggactgg tcctggcatc acgtcttcct ttgccgtgac
      841 cacgtgatca ccaggtgctc tcttaggggg ctggggggag ccagtggagc ccccaagccc
      901 cagcggcttg cta
//
