LOCUS       sequence-42685-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Mar 17. 21(6):873-80."
COMMENT     Sequence Label: Ubiquitin I44A
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             130..357
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLAF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             384..401
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             411..428
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(500..518)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      514..561
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(736..741,742..758,759..764))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(791..809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agaggatcca tgcagatctt cgtcaagacg ttaaccggta aaaccataac tctggaagtt
      181 gaaccatccg ataccatcga aaacgttaag gctaaaattc aagacaagga aggaattcca
      241 cctgatcaac aaagattggc atttgccggt aagcagctcg aggacggtag aacgctgtct
      301 gattacaaca ttcagaagga gtcgacctta catcttgtct taagactaag aggtggttga
      361 tgaggtaccc catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
      421 gcggcagcca tatgctcgag gatcgtcgac gatccggctg ctaacaaagc ccgaaaggaa
      481 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      541 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc cgcaagaggc
      601 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
      661 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
      721 gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga
      781 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      841 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      901 ttc
//
