LOCUS       sequence-426853-       10671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express OsTIR1.F74A mutant with mCherry tag for AID2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Li S, Wang Y, van der Stoel M, Zhou X, Madhusudan S, Kanerva K, 
            Nguyen VD, Eskici N, Olkkonen VM, Zhou Y, Raivio T, Ikonen E
  TITLE     HiHo-AID2: boosting homozygous knock-in efficiency enables robust 
            generation of human auxin-inducible degron cells.
  JOURNAL   Genome Biol. 2024 Feb 26;25(1):58. doi: 10.1186/s13059-024-03187-w.
  PUBMED    38409044
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2024-02-26"; volume: "25(1):58. doi"; pages: " 
            10.1186/s13059-024-03187-w"
COMMENT     Sequence Label: pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro
FEATURES             Location/Qualifiers
     source          1..10671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(135..152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(306..894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(386..405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1065..1856,1857..1925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1688..1707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1926..2030)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2057..2512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2144..2163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2354..2375
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    3029..3832
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    3858..3906
                     /note="synthetic polyadenylation signal"
     misc_feature    3920..4011
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3960..3979
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4023..5185
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4254..5176
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5133..5153
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     intron          5224..5356
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5339..5358
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5401..5419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7186..7893
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7328..7346)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7596..7615
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7912..8478
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7928..8480
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8072..8089)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8299..8318
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8480..9082
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8979..8999
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9316..9397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9353..9372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    9425..10261
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
       61 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
      121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2041 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg
     2521 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2581 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     2641 gtcgacctac tctcttccgc attggagtcg ctttaactgg ccctggcttt ggcagcctgt
     2701 gctgacccat gcagtcctcc ttaccatccc tccctcgact tcccctcttc cgatgttgag
     2761 cccctccagc cggtcctgga ctttgtctcc ttccctgccc tgccctctcc tgaacctgag
     2821 ccagctccca tagctcagtc tggtctatct gcctggccct ggccattgtc actttgcgct
     2881 gccctcctct cgcccccgag tgcccttgct gtgccgccgg aactctgccc tctaacgctg
     2941 ccgtctctct cctgagtccg gaccactttg agctctactg gcttctgcgc cgcctctggc
     3001 ccactgtttc cccttcccag gcaggtcctg ctttctctga cctgcattct ctcccctggg
     3061 cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct ggcccagatc
     3121 cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc ttgctctctg
     3181 ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc tgcaccacgt
     3241 gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct ctcctccctc
     3301 acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt ctggggcctg
     3361 tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg cgtcccgcct
     3421 ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag taccccgtct
     3481 ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc ccgttctcct
     3541 gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc cccttacctc
     3601 tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg tccgagagct
     3661 cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag catgtttgct
     3721 gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg gccggttaat
     3781 gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg gcgcatgcgt
     3841 ggaattatca cctcgagaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     3901 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     3961 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tactagtcgt
     4021 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4081 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4141 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4201 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4261 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4321 ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc
     4381 ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4441 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4501 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     4561 aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     4621 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     4681 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     4741 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     4801 aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg
     4861 agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc
     4921 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt
     4981 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     5041 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     5101 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     5161 ttttcttcca tttcaggtgt cgtgaacacg ggctgcagaa gttggtcgtg aggcactggg
     5221 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     5281 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     5341 gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact
     5401 taatacgact cactataggc tagcgctacc ggactcagat ctaccatgac ctacttccct
     5461 gaggaggtgg tggagcacat cttctccttt ctgccagccc agagggaccg caacacagtg
     5521 tctctggtgt gcaaagtgtg gtacgagatc gagcgcctgt ccaggagagg cgtgttcgtg
     5581 ggcaactgtt atgccgtgcg ggcaggaaga gtggcagcaa ggtttccaaa tgtgcgggcc
     5641 ctgaccgtca aggggaaacc ccacgccgcc gactttaacc tggtgccacc tgattgggga
     5701 ggatatgcag gaccatggat cgaggcagca gcaaggggat gccacggact ggaggagctg
     5761 aggatgaagc gcatggtggt gagcgacgag tccctggagc tgctggcacg gagcttccca
     5821 cggtttagag ccctggtgct gatctcttgc gagggcttta gcaccgatgg actggcagca
     5881 gtggcaagcc actgtaagct gctgcgggag ctggacctgc aggagaacga ggtggaggat
     5941 cggggcccta gatggctgtc ctgcttccca gactcttgta caagcctggt gtccctgaac
     6001 tttgcctgca tcaagggcga agtgaatgcc ggcagcctgg agagactggt gtccaggtct
     6061 cctaacctga ggtccctgcg cctgaatcgg agcgtgtccg tggacaccct ggccaagatc
     6121 ctgctgagaa caccaaacct ggaggatctg ggcaccggca atctgacaga cgatttccag
     6181 accgagagct actttaagct gacatccgcc ctggagaagt gtaagatgct gaggtctctg
     6241 agcggattct gggatgcatc ccccgtgtgc ctgtctttta tctaccctct gtgcgcccag
     6301 ctgaccggcc tgaatctgtc ttatgccccc accctggacg cctctgatct gacaaagatg
     6361 atcagcagat gcgtgaagct gcagaggctg tgggtgctgg actgtatcag cgataagggc
     6421 ctgcaggtgg tggccagctc ctgcaaggac ctgcaggagc tgagagtgtt ccccagcgac
     6481 ttctacgtgg ccggctattc cgccgtgaca gaggagggac tggtggccgt gtctctggga
     6541 tgccctaagc tgaacagcct gctgtatttc tgtcaccaga tgaccaatgc cgccctggtg
     6601 acagtggcca agaactgccc aaatttcacc aggtttcgcc tgtgcatcct ggagccaggc
     6661 aagcccgacg tggtgacatc ccagcccctg gatgagggct ttggcgccat cgtgagagag
     6721 tgcaagggcc tgcagaggct gtccatctct ggcctgctga ccgacaaggt gttcatgtac
     6781 atcggcaagt atgccaagca gctggagatg ctgtccatcg cctttgccgg cgactctgat
     6841 aagggcatga tgcacgtgat gaacggctgt aagaatctga gaaagctgga gatcagggac
     6901 tcccctttcg gcgatgccgc cctgctgggc aactttgccc gctacgagac catgcggtct
     6961 ctgtggatgt ctagctgcaa tgtgacactg aagggctgtc aggtgctggc ctctaagatg
     7021 ccaatgctga acgtggaagt gatcaacgag agggacggca gcaacgagat ggaggagaat
     7081 cacggcgatc tgcccaaggt ggagaagctg tacgtgtata ggaccacagc aggagcaagg
     7141 gacgatgcac ctaacttcgt gaagatcctg ggcggatctg gcggggtgag caagggcgag
     7201 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     7261 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     7321 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     7381 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     7441 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     7501 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     7561 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     7621 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     7681 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     7741 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     7801 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     7861 tccaccggcg gcatggacga gctgtacaag tagcggccgc tctagcccaa ttccgccccc
     7921 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     7981 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     8041 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     8101 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     8161 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     8221 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     8281 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     8341 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     8401 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     8461 tgaaaaacac gatgataata tgggcaccga gtacaagccc acggtgcgcc tcgccacccg
     8521 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     8581 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     8641 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     8701 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     8761 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     8821 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     8881 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8941 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9001 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9061 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     9121 cgcacgaccc catggctccg accgaagcca cccggggcgg ccccgccgac cccgcacccg
     9181 cccccgaggc ccaccgactc tagctagagg atcataatca gccataccac atttgtagag
     9241 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     9301 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccatg
     9421 catcactagg gacaggattg gtgacagaaa agccccatcc ttaggcctcc tccttcctag
     9481 tctcctgata ttgggtctaa cccccacctc ctgttaggca gattccttat ctggtgacac
     9541 acccccattt cctggagcca tctctctcct tgccagaacc tctaaggttt gcttacgatg
     9601 gagccagaga ggatcctggg agggagagct tggcaggggg tgggagggaa gggggggatg
     9661 cgtgacctgc ccggttctca gtggccaccc tgcgctaccc tctcccagaa cctgagctgc
     9721 tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc tgcagcttcc ttacacttcc
     9781 caagaggaga agcagtttgg aaaaacaaaa tcagaataag ttggtcctga gttctaactt
     9841 tggctcttca cctttctagt ccccaattta tattgttcct ccgtgcgtca gttttacctg
     9901 tgagataagg ccagtagcca gccccgtcct ggcagggctg tggtgaggag gggggtgtcc
     9961 gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc
    10021 ctctactccc tttctctttc tccatccttc tttccttaaa gagtccccag tgctatctgg
    10081 gacatattcc tccgcccaga gcagggtccc gcttccctaa ggccctgctc tgggcttctg
    10141 ggtttgagtc cttggcaagc ccaggagagg cgctcaggct tccctgtccc ccttcctcgt
    10201 ccaccatctc atgcccctgg ctctcctgcc ccttccctac aggggttcct ggctctgctc
    10261 ttcagactga gccccgttcc cctgcatccc cgttcccctg catccccctt cccctgcatc
    10321 ccccagaggc cccaggccac ctacttggcc tggaccccac gagaggccac cccagccctg
    10381 tctaccaggc tgccttttgg gtggattctc ctccaactgt ggggtgactg cttggcaaac
    10441 tcactcttcg gggtatccca ggaggcctgg agcattgggg tgggctgggg ttcagagagg
    10501 agggattccc ttctcaggtt acgtggccaa gaagcagggg agctgggttt gggtcaggtc
    10561 tgggtgtggg gtgaccagct tatgctgttt gcccaggaca gcctagtttt agcgctgaaa
    10621 ccctcagtcc taggaaaaca gggatggttg gtcactgtct ctgggctcga c
//
