LOCUS       sequence-426862-        4878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Cre-dependent expression of alpha synuclein (human gene 
            containing A53T mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Steinkellner T, Conrad WS, Kovacs I, Rissman RA, Lee EB, Trojanowski
            JQ, Freyberg Z, Roy S, Luk KC, Lee VM, Hnasko TS
  TITLE     Dopamine neurons exhibit emergent glutamatergic identity in 
            Parkinson's disease.
  JOURNAL   Brain. 2022 Apr 29;145(3):879-886. doi: 10.1093/brain/awab373.
  PUBMED    35258081
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Brain"; date: "2022-04-29- 29"; volume: "145"; issue: "3"; pages: 
            "879-886"
COMMENT     Sequence Label: pAAV-Syn1-DIO-Snca (human A53T)
FEATURES             Location/Qualifiers
     source          1..4878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    153..360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     349..366
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    401..989
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             507..518
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     916..936
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(1013..1046)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1057..1090)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1548..1581
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1592..1625
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1658..2105)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     repeat_region   2141..2281
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2141..2270
                     /label=AAV2 ITR
     rep_origin      2356..2811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2443..2462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2653..2674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2828..2847)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2947..2969
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3007..3025)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3093..3197
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3198..3266,3267..4058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3416..3435)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4229..4817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4718..4737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgctc ggtccgcacg tgtccccagc atgcctgcta ttgtcttccc
      181 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
      241 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
      301 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
      361 tcgaggcaga tcgatctctc gagcagcgct cggtatcgat gcggggaggc ggcccaaagg
      421 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
      481 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
      541 ccgcgcagaa tccaggtggc aacataggcg agcagccaag gaaaggacga tgatttcccc
      601 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
      661 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
      721 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
      781 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
      841 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
      901 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
      961 tcacaaattt tgtaatccag aggttgatta tcgataagct tgatgatctt cgataacttc
     1021 gtataggata ctttatacga agttattgcg attcggataa cttcgtatag catacattat
     1081 acgaagttat caaggccggc caggttggcg cgccatggat gtattcatga aaggactttc
     1141 aaaggccaag gagggagttg tggctgctgc tgagaaaacc aaacagggtg tggcagaagc
     1201 agcaggaaag acaaaagagg gtgttctcta tgtaggctcc aaaaccaagg agggagtggt
     1261 gcatggtgtg acaacagtgg ctgagaagac caaagagcaa gtgacaaatg ttggaggagc
     1321 agtggtgacg ggtgtgacag cagtagccca gaagacagtg gagggagcag ggagcattgc
     1381 agcagccact ggctttgtca aaaaggacca gttgggcaag aatgaagaag gagccccaca
     1441 ggaaggaatt ctggaagata tgcctgtgga tcctgacaat gaggcttatg aaatgccttc
     1501 tgaggaaggg tatcaagact acgaacctga agcctaaggc gcgccaaata acttcgtata
     1561 aagtatccta tacgaagtta ttgcgattcg gataacttcg tataatgtat gctatacgaa
     1621 gttatcgacg tcgacctgaa gggtaccgtc cacgcgtctg cgctctcagg cacgacacga
     1681 ctcctccgct gcccaccgca gactgaggca gcgctgagtc gccggcgccg cagcgcagat
     1741 ggtcgcgccc gtgcccccct atctcgcgcc tcgcgtggtg cggtccggct gggccggcgg
     1801 cggcgcggac gcgaccaagg tggccgggaa ggggagtttg cgggggaccg gcgagtgacg
     1861 tcagcgcgcc ttcagtgctg aggcggcggt ggcgcgcgcc gccaggcggg ggcgaaggca
     1921 ctgtccgcgg tgctgaagct ggcagtgcgc acgcgcctcg ccgcatcctg tttcccctcc
     1981 ccctctctga taggggatgc gcaatttggg gaatgggggt tgggtgcttg tccagtgggt
     2041 cggggtcggt cgtcaggtag gcacccccac cccgcctcat cctggtccta aaacccactt
     2101 gcactcatac gcagggccct ctgcagacgc gtgcggccgc aggaacccct agtgatggag
     2161 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     2221 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     2281 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     2341 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2401 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     2461 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2521 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     2581 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2641 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     2701 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     2761 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     2821 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     2881 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2941 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     3001 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3061 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3121 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3181 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3241 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3301 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3361 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3421 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3481 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3541 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3601 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3661 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3721 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3781 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3841 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3901 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3961 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4021 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4081 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4141 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4201 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4261 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4321 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4621 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4801 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4861 ggccttttgc tcacatgt
//
