LOCUS       sequence-426867-        4790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning donor vector containing DDX3X ASD mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Allan, Loewen Labs
  TITLE     Allan and Loewen Labs SFARI funded autism variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DDX3X - E186A in pENTR
FEATURES             Location/Qualifiers
     source          1..4790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..69
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     61..78
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     62..78
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    94..193
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2194..2293)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2310..2329)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2311..2329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2334..2350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2334..2350)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2781..3572
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3665..4253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4154..4173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4407..4424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4583..4610)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4702..4788)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
       61 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
      121 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
      181 aaaaaagcag gctccggcac catgagtcat gtggcagtgg aaaatgcgct cgggctggac
      241 cagcagtttg ctggcctaga cctgaactct tcagataatc agagtggagg aagtacagcc
      301 agcaaagggc gctatattcc tcctcattta aggaaccgag aagctactaa aggtttctac
      361 gataaagaca gttcagggtg gagttctagc aaagataagg atgcgtatag cagttttgga
      421 tctcgtagtg attcaagagg gaagtctagc ttcttcagtg atcgtggaag tggatcaagg
      481 ggaaggtttg atgatcgtgg acggagtgat tacgatggca ttggcagccg tggtgacaga
      541 agtggctttg gcaaatttga acgtggtgga aacagtcgct ggtgtgacaa atcagatgaa
      601 gatgattggt caaaaccact cccaccaagt gaacgcttgg aacaggaact cttttctgga
      661 ggcaacactg ggattaattt tgagaaatac gatgacattc cagttgaggc aacaggcaac
      721 aactgtcctc cacatattga aagtttcagt gatgttgcga tgggagaaat tatcatggga
      781 aacattgagc ttactcgtta tactcgccca actccagtgc aaaagcatgc tattcctatt
      841 atcaaagaga aaagagactt gatggcttgt gcccaaacag ggtctggaaa aactgcagca
      901 tttctgttgc ccatcttgag tcagatttat tcagatggtc caggcgaggc tttgagggcc
      961 atgaaggaaa atggaaggta tgggcgccgc aaacaatacc caatctcctt ggtattagca
     1021 ccaacgagag agttggcagt acagatctac gaggaagcca gaaaattttc ataccgatct
     1081 agagttcgtc cttgcgtggt ttatggtggt gccgatattg gtcagcagat tcgagacttg
     1141 gaacgtggat gccatttgtt agtagccact ccaggacgtc tagtggatat gatggaaaga
     1201 ggaaagattg gattagactt ttgcaaatac ttggtgttag atgaagctga tcggatgttg
     1261 gatatggggt ttgagcctca gattcgtaga atagtcgaac aagatactat gcctccaaag
     1321 ggtgtccgcc acactatgat gtttagtgct acttttccta aggaaataca gatgctggct
     1381 cgtgatttct tagatgaata tatcttcttg gctgtaggaa gagttggctc tacctctgaa
     1441 aacatcacac agaaagtagt ttgggtggaa gaatcagaca aacggtcatt tctgcttgac
     1501 ctcctaaatg caacaggcaa ggattcactg accttagtgt ttgtggagac caaaaagggt
     1561 gcagattctc tggaggattt cttataccat gaaggatacg catgtaccag catccatgga
     1621 gaccgttctc agagggatag agaagaggcc cttcaccagt tccgctcagg aaaaagccca
     1681 attttagtgg ctacagcagt agcagcaaga ggactggaca tttcaaatgt gaaacatgtt
     1741 atcaattttg acttgccaag tgatattgaa gaatatgtac atcgtattgg tcgtacggga
     1801 cgtgtaggaa accttggcct ggcaacctca ttctttaacg agaggaacat aaatattact
     1861 aaggatttgt tggatcttct tgttgaagct aaacaagaag tgccgtcttg gttagaaaac
     1921 atggcttatg aacaccacta caagggtagc agtcgtggac gttctaagag tagcagattt
     1981 agtggagggt ttggtgccag agactaccga caaagtagcg gtgccagcag ttccagcttc
     2041 agcagcagcc gcgcaagcag cagccgcagt ggcggaggtg gccacggtag cagcagagga
     2101 tttggtggag gtggctatgg aggcttttac aacagtgatg gatatggagg aaattataac
     2161 tcccaggggg ttgactggtg gggtaactga tagacccagc tttcttgtac aaagttggca
     2221 ttataaaaaa taattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2281 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2341 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2401 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2461 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2521 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2581 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2641 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2701 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2761 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2821 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2881 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2941 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3001 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3061 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3121 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3181 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3241 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3301 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3361 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3421 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3481 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3541 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3601 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4441 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4501 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4561 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4621 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4681 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4741 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
//
