LOCUS       sequence-426886-        4193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning donor vector containing EEF1A2 ASD mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Allan, Loewen Labs
  TITLE     Allan and Loewen Labs SFARI funded autism variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EEF1A2 - D252H in pENTR
FEATURES             Location/Qualifiers
     source          1..4193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..69
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     61..78
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     62..78
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    94..193
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1597..1696)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1713..1732)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1714..1732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1737..1753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1737..1753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2184..2975
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3068..3656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3557..3576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3810..3827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3986..4013)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4105..4191)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
       61 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
      121 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
      181 aaaaaagcag gctccggcac catgggcaag gagaagaccc acatcaacat cgtggtcatc
      241 ggccacgtgg actccggaaa gtccaccacc acgggccacc tcatctacaa atgcggaggt
      301 attgacaaaa ggaccattga gaagttcgag aaggaggcgg ctgagatggg gaagggatcc
      361 ttcaagtatg cctgggtgct ggacaagctg aaggcggagc gtgagcgcgg catcaccatc
      421 gacatctccc tctggaagtt cgagaccacc aagtactaca tcaccatcat cgatgccccc
      481 ggccaccgcg acttcatcaa gaacatgatc acgggtacat cccaggcgga ctgcgcagtg
      541 ctgatcgtgg cggcgggcgt gggcgagttc gaggcgggca tctccaagaa tgggcagacg
      601 cgggagcatg ccctgctggc ctacacgctg ggtgtgaagc agctcatcgt gggcgtgaac
      661 aaaatggact ccacagagcc ggcctacagc gagaagcgct acgacgagat cgtcaaggaa
      721 gtcagcgcct acatcaagaa gatcggctac aacccggcca ccgtgccctt tgtgcccatc
      781 tccggctggc acggtgacaa catgctggag ccctccccca acatgccgtg gttcaagggc
      841 tggaaggtgg agcgtaagga gggcaacgca agcggcgtgt ccctgctgga ggccctggac
      901 accatcctgc cccccacgcg ccccacggac aagcccctgc gcctgccgct gcagcacgtg
      961 tacaagattg gcggcattgg cacggtgccc gtgggccggg tggagaccgg aatcctgcgg
     1021 ccgggcatgg tggtgacctt tgcgccagtg aacatcacca ctgaggtgaa gtcagtggag
     1081 atgcaccacg aggctctgag cgaagctctg cccggcgaca acgtcggctt caatgtgaag
     1141 aacgtgtcgg tgaaggacat ccggcggggc aacgtgtgtg gggacagcaa gtctgacccg
     1201 ccgcaggagg ctgctcagtt cacctcccag gtcatcatcc tgaaccaccc ggggcagatt
     1261 agcgccggct actccccggt catcgactgc cacacagccc acatcgcctg caagtttgcg
     1321 gagctgaagg agaagattga ccggcgctct ggcaagaagc tggaggacaa ccccaagtcc
     1381 ctgaagtctg gagacgcggc catcgtggag atggtgccgg gaaagcccat gtgtgtggag
     1441 agcttctccc agtacccgcc tctcggccgc ttcgccgtgc gcgacatgag gcagacggtg
     1501 gccgtaggcg tcatcaagaa cgtggagaag aagagcggcg gcgccggcaa ggtcaccaag
     1561 tcggcgcaga aggcgcagaa ggcgggcaag tgatagaccc agctttcttg tacaaagttg
     1621 gcattataaa aaataattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa
     1681 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     1741 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     1801 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     1861 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1921 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1981 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2041 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2101 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2161 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     2221 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2281 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2341 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2401 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2461 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2521 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2581 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2641 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2701 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2761 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2821 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2881 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2941 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3001 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3061 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3181 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3421 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3661 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3721 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3781 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3841 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3901 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3961 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4021 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4081 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4141 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gat
//
