LOCUS       sequence-426910-        5194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of USP46.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ng VH, Spencer Z, Neitzel LR, Nayak A, Loberg MA, Shen C, Kassel SN,
            Kroh HK, An Z, Anthony CC, Bryant JM, Lawson A, Goldsmith L, 
            Benchabane H, Hansen AG, Li J, D'Souza S, Lebensohn AM, Rohatgi R, 
            Weiss WA, Weiss VL, Williams C, Hong CC, Robbins DJ, Ahmed Y, Lee E
  TITLE     The USP46 complex deubiquitylates LRP6 to promote Wnt/beta-catenin 
            signaling.
  JOURNAL   Nat Commun. 2023 Oct 5;14(1):6173. doi: 10.1038/s41467-023-41836-z.
  PUBMED    37798301
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-5"; volume: "14(1):6173. doi"; pages: " 
            10.1038/s41467-023-41836-z"
COMMENT     Sequence Label: Human USP46
FEATURES             Location/Qualifiers
     source          1..5194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      101..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(188..207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2594..2615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2630..2635,2636..2653,2654..2660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2668..2684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2673..2695
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2692..2708
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2692..2708
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2727..2747
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2729..2747
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2860..2994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2897..2916)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2951..2970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2999..3018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2999..3017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4187..4205)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4188..4205)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(4240..5194,1..23))
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 catatacgcc atattgaatt ggcggtcgac tggcgtaata gcgaagaggc ccgcaccgat
       61 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
      121 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      181 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      241 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      301 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      361 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agattctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttataa tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2521 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2581 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2641 tgctcccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2701 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     2761 gtaccgggcc caatgcattg gcgccgcggc cgcgaattaa aaaacctccc acacctcccc
     2821 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2941 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctacgta
     3001 atacgactca ctatagggtc tagatggcgc gccttactct cttgactgat agaataaaat
     3061 atatccagat tctgaatttt ttgatatatc tgacgtcagg ccatagaatt cttcaatagc
     3121 ttgagcatct attttctcta caatgtcatc atcaaacaaa agccagaagc cgtgactttt
     3181 cacaatagtg atataatgcc cacgattagg accactgcca cagtgaacga ccaccgcaac
     3241 caagtcatac atgcggtcca ggttcactgc atcactggag gtgttgaaga gccggagttc
     3301 cagagggaag accacacggt aagacagctt ggtgtatctg tgcagctgct ccatgtactt
     3361 gaaccgcttt aggtgcaggg ccaagatcat gggcagcttt tttaccctca tccttttctg
     3421 ggcttcttgt ttgctgcagc atgtttcaca ataatatttt tgttcactac acagtgtttc
     3481 tgtgttgctg aagtctctta gacagtgggt aatggatgta ttctgctcca catcaacaga
     3541 aaggtcaaga aaatcttcat ctttgctact aacagtttca cagttcaagc atcgagtttc
     3601 attggtaagc gttccctgaa aaatctcatg gacccaggtg agttctggtt tattattttc
     3661 cgcaggttcg ttcatgttgc cattttttaa ttttccattt tgtttttcct gtttcttctc
     3721 ctcctgaagg atgtccgcaa tagtgtttag caaataattt aaaaattcat gagcatcctg
     3781 ctgcatgtag ttatcaaaga gatcattctc ttttctcagc cttgaaatga acttctttgg
     3841 tgggatgacg ccaaccttct tcttctgtgt ggcaatgctg tggaaaaggt ccgccaggca
     3901 cgtcagcaag ttttccttct tcttttgctg ggccttgtat gccaacacat tctcccggaa
     3961 tggacggcag aagtacaatg cctgaagcac ggagttacag tagcatgtgt ttccaaaatt
     4021 gaccaatccg aaatagtgtt cattgattgg aaactgctct ggaccaatgt ctttttccag
     4081 agcagaggca ttggtgccca tattacagat ggaggcgatg tttcggacag tcattggccg
     4141 gccatcgatg aattcggatc ctgcaaaaag aacaagtagc ttgtattcta tagtgtcacc
     4201 taaatcaagc ttgctccgac gtccccaggc agaatggcgg ttccctaaac gagcattgct
     4261 tatatagacc tcccattagg cacgcctacc gcccatttac gtcaatggaa cgcccatttg
     4321 cgtcattgcc cctccccatt gacgtcaatg gggatgtact tggcagccat cgcgggccat
     4381 ttaccgccat tgacgtcaat gggagtactg ccaatgtacc ctggcgtact tccaatagta
     4441 atgtacttgc caagttacta ttaatagata ttgatgtact gccaagtggg ccatttaccg
     4501 tcattgacgt caataggggg cgtgagaacg gatatgaatg ggcaatgagc catcccattg
     4561 acgtcaatgg tgggtggtcc tattgacgtc aatgggcatt gagccaggcg ggccatttac
     4621 cgtaattgac gtcaatgggg gaggcgccat atacgtcaat aggaccgccc atatgacgtc
     4681 aataggaaag atcatatata gagaccattg acgtcaatgg gggagtggct atgggcggta
     4741 ttaggaagcc ccatatatgg tatatgggac cgcccattgg gaggggctat ctacgtcaat
     4801 aggaaaaccc atatatggaa tactatatgg catagggcca atacatagta ttgaacctgg
     4861 ccaatagcca tattggcata gggccatatt ggatattgcc tatatattga tcctggcata
     4921 tagccaatat ggccgccatt attggcacca tgccaattca atatggcgga cttggcacag
     4981 ggccaagacc cctcctcaaa cttggcaccg tgccaagtag acccctcccc agttggcaca
     5041 gtgctaggtc cgccatattg aattggcatg gtgccaagtc cgccatattg aattggctgg
     5101 ggtccagatc caccatattg aattggcacg agtccatata cgccatattg aattggcaca
     5161 ggtccagatc caccatattg aattggcatg agtc
//
