LOCUS       sequence-42692-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Curtis C, Hemmeryckx B, Haataja L, Senadheera D, Groffen J, 
            Heisterkamp N
  TITLE     Scambio, a novel guanine nucleotide exchange factor for Rho.
  JOURNAL   Mol Cancer. 2004 Apr 23;3:10.
  PUBMED    15107133
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Cancer."; date: "2004-04-23"; volume: "3"; pages: "10"
COMMENT     Sequence Label: pIRESNEO-Scambio-HA
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             138..164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             144..170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctctctggnt nnntagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatcgatat ctgcggccgc gtcgacggaa
      121 ttcggatctc tatggcttac ccatacgatg ttccagatta cgctagcttg ggtggtcata
      181 tggccatgga gcctgagcca gtggaggact gtgtgcagag cactctcgcc gccctgtatc
      241 caccctttga ggcaacagcc cccaccctgt tgggccaggt gttccaggtg gtggagagga
      301 cttatcggga ggacgcactg aggtacacgc tggacttcct ggtaccagcc aagcacctgc
      361 ttgccaaggt ccagcaggaa gcctgtgccc aatacagtgg attcctcttc ttccatgagg
      421 ggtggccgct ctgcctgcat gaacaggtgg tggtgcagct agcagcccta ccctggcaac
      481 tgctgcgccc aggagacttc tatctgcagg tggtgccctc agctgcccaa gcaccccgac
      541 tagcactcaa gtgtctggcc cctgggggtg ggcgggtgca ggaggttcct gtgcccaatg
      601 aggcttgtgc ctacctattc acacctgagt ggctacaagg catcaacaag gaccggccaa
      661 caggtcgcct cagtacctgc ctactgtctg cgccctctgg gattcagcgg ctgccctggg
      721 ctgagctcat ctgtccacga tttgtgcaca aagagggcct catggttgga catcagccaa
      781 gtacactgcc cccaga
//
