LOCUS       sequence-42693-e         441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N
  TITLE     Abr and Bcr, two homologous Rac GTPase-activating proteins, control 
            multiple cellular functions of murine macrophages.
  JOURNAL   Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.
  PUBMED    17116687
REFERENCE   2  (bases 1 to 441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2007-02"; volume: "27(3)"; pages: "899-911. Epub 2006
            Nov 20"
COMMENT     Sequence Label: pCDNA3.1Xpress-Bcr-HisB
FEATURES             Location/Qualifiers
     source          1..441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             139..171
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactnn
       61 ntatagggag acccaagctg gctagcgttt aaacttaagc ttaccatggg gggttctcat
      121 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      181 tactacgatg acgataaggt acctaaggat ccagtgtggt ggaattcggt ggacccggtg
      241 ggcttcgcgg aggcgtggaa ggcgcagttc ccggactcag atcccccgcg catggagctg
      301 cgctcagtgg gcgacttcna gcaggagctg gagcgctgca aggcctccat tcggcgcctg
      361 gatcaggagg tgaaccagga gcgcttccgc atgatctacc tgcngacctt gttggcctca
      421 gaaaacaaca gctatgaccg g
//
