LOCUS       sequence-42694-e        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fluorescent reporter of AMPK activity and cellular energy stress.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Tsou P, Zheng B, Hsu CH, Sasaki AT, Cantley LC
  TITLE     A fluorescent reporter of AMPK activity and cellular energy stress.
  JOURNAL   Cell Metab. 2011 Apr 6;13(4):476-86.
  PUBMED    21459332
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-04-6"; volume: "13(4)"; pages: "476-86"
COMMENT     Sequence Label: ampkar
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(15..23,24..38)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(45..47,48..50,51..563)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1251..1985
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 agcaaatggg tcgggatctg tacgacgatg acgataagga tcccatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaggttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      241 cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacgtccagg agcgtaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      481 acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggcccact
      541 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      661 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      721 ccgccgcccg catgcataag ttttctcaag aacagatcgg cgaaaacatt gtgtgcaggg
      781 tcatttgtac cacgggtcaa attcccatcc gagatttgtc agctgatatt tcacaagtgc
      841 ttaaggaaaa acgatccata aagaaagttt ggacatttgg tagaaaccca gcctgtgact
      901 atcatttagg aaacatttca agactgtcaa ataagcattt ccaaatacta ctaggagaag
      961 acggtaacct tttattgaat gacatttcca ctaatgggac ctggttaaat gggcaaaaag
     1021 tcgagaagaa cagcaatcag ttactgtctc aaggtgatga aataaccgtt ggtgtaggcg
     1081 tggaatcaga tattttatct ctggtcattt tcataaacga caaatttaag cagtgcctcg
     1141 agcagaacaa agttgatcgc tctgcaggta agccaggcag cggcgagggc agcaccaaga
     1201 tgcgtcgtgt cgccaccctg gtggacctcg gcaccggcgg cagcgagctc atgggcggcg
     1261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1321 ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
     1381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1441 tgtacaaggg aggtaccggt ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg
     1501 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1561 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
     1621 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc cttcagtgct
     1681 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1741 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1801 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1861 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1921 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1981 tcgagtaa
//
