LOCUS       sequence-42698-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Raingeaud J, Whitmarsh AJ, Barrett T, Derijard B, Davis RJ
  TITLE     MKK3- and MKK6-regulated gene expression is mediated by the p38 
            mitogen-activated protein kinase signal transduction pathway.
  JOURNAL   Mol Cell Biol. 1996 Mar . 16(3):1247-55.
  PUBMED    8622669
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1996 Mar . 16(3):1247-55."
COMMENT     Sequence Label: pCDNA3-Flag MKK6(glu)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcgactct ctggctacta gagacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttcggcagcc aacatggact ataaggacga tgatgacaaa
      121 tctcagtcga aaggcaagaa gcgaaaccct ggccttaaaa ttccaaaaga agcatttgaa
      181 caacctcaga ccagttccac accacctaga gatttagact ccaaggcttg catttctatt
      241 ggaaatcaga actttgaggt gaaggcagat gacctggagc ctataatgga actgggacga
      301 ggtgcgtacg gggtggtgga gaagatgcgg cacgtgccca gcgggcagat catggcagtg
      361 aagcggatcc gagccacagt aaatagccag gaacagaaac ggctactgat ggatttggat
      421 atttccatga ggacggtgga ctgtccattc actgtcacct tttatggcgc actgtttcgg
      481 gagggtgatg tgtggatctg catggagctc atggatacat cactagataa attctacaaa
      541 caagttattg ataaaggcca gacaattcca gaggacatct tagggaaaat agcagtttct
      601 attgtaaaag cattagaaca tttacatagt aagctgtctg tcattcacag agacgtcaag
      661 ccttctaatg tactcatcaa tgctctcggt caagtgaaga tgtgcgattt tggaatcagt
      721 ggctacttgg tggacgaggt tgctaaagaa attgatgcag gttgcaaacc atacatggcc
      781 cctgaaagaa taaacccaga gctcaaccag aagggataca gtgtgaagtc
//
